Garbage leachate toxicity evaluation method

Abstract

The invention relates to the technical field of wastewater detection, and in particular relates to a garbage leachate toxicity evaluation method. The garbage leachate toxicity evaluation method comprises the following steps of (1) preparing test sample liquid from garbage leachate; (2) performing quantitative in-vitro biotoxicity evaluation on the test sample liquid through a recombined gene spore; (3) exposing early developed fish serving as a tested biological material in the test sample liquid, checking growing development reproduction conditions and genetic transcription change conditions of the fish, so as to obtain a real quantitative detection result of garbage leachate toxicity; and (4) exposing scenedesmus obliquus-moina macrocopa straus-zebra fish serving as a tested simulated ecological system in the test sample liquid, and evaluating a biological enrichment and generative transmission effect of garbage leachate toxicity substances, so as to obtain a relevant detection result. The method disclosed by the invention has multiple advantages of quickness, high efficiency, precision in quantification, truth, reliability, multi-layer evaluation and the like, and has the extremely large practical value and the wide market prospect.

Description

technical field [0001] The invention relates to the technical field of wastewater detection, in particular to a method for evaluating the toxicity of landfill leachate, which is used for evaluating the ecological risk of landfill leachate and the harm to human health. Background technique [0002] Landfill leachate refers to a liquid with a high concentration of organic or inorganic components produced during the process of stacking and landfilling of garbage due to biochemical degradation such as compaction and fermentation, and at the same time under the seepage of precipitation and groundwater. [0003] With the improvement of the level of economic development and the acceleration of urbanization, the amount of garbage is increasing day by day, and the pollution of landfill leachate is becoming more and more serious, which has aroused widespread concern from the government and the public. How to realize the reduction, harmlessness and recycling of urban garbage in our cou...

AI Technical Summary

Problems solved by technology

At the same time, with the use and disposal of a large number of drugs, such as contraceptives, steroid drugs, antibiotics, etc., they will eventually enter the environment with garbage, posing risks to the ecological environment and human health

[0005] At present, chemical analysis methods for single toxic compounds in landfill leachate are not uncommon, and biological toxicity evaluation data are also reported.

However, there is a lack of quantitative evaluation methods for the combined biological toxicity of landfill leachate with complex components and a wide variety of toxic substances