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Scenedesmus obliquus strain and separation purifying method, cultivation method and application thereof

A cultivation method, the technology of Scenedesmus obliques, which is applied in the field of microorganisms and bioenergy, to achieve the effects of reducing pollution, reducing costs, and saving costs

Active Publication Date: 2016-09-28
HENAN UNIV OF URBAN CONSTR +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the cultivation and extraction method of oil-rich algae and its related applications have been disclosed, but for its rich application, it is only a drop in the bucket
Therefore, research on this type of technology is still continuing to deepen and expand, and there are still a large number of oil-rich algae species that need to be discovered, effective cultivation and separation, and in-depth application research.

Method used

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  • Scenedesmus obliquus strain and separation purifying method, cultivation method and application thereof
  • Scenedesmus obliquus strain and separation purifying method, cultivation method and application thereof
  • Scenedesmus obliquus strain and separation purifying method, cultivation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0053] A Scenedesmus obliquus strain SoHNCJ2, the preservation number is CCTCC NO: M2016253, the preservation date is May 6, 2016, and the preservation unit is the China Center for Type Culture Collection.

Embodiment 2

[0055] A method for separating and purifying Scenedesmus obliquus strain SoHNCJ2, comprising the steps of:

[0056] 1) Collect water samples from multiple points in Pingxi Lake Reservoir, Xincheng District, Pingdingshan City. Dilute the collected water samples and evenly spread them on a BG11 medium plate with 1.5-2% agar, and place them in an incubator for continuous light cultivation;

[0057] 2) After a single algae grows on the plate, use a toothpick to pick a single algae and drop it on the BG11 plate to draw a short line for culture;

[0058] 3) The algae species cultivated by dashed lines are separated and sterilized by inoculating the Z-shaped line with the inoculation needle, and then the algae species are further purified by diluting and spreading on the plate;

[0059] 4) Picking a single algae colony to draw a short line and picking a short line algae colony to draw a Z line alternately for 2 to 4 times to obtain a relatively pure single algae colony.

[0060] The...

Embodiment 3

[0076] This example is a large-scale cultivation method of the Scenedesmus obliquus strain SoHNCJ2 and a method for producing biodiesel by translipidation.

[0077] 1. Culture of Algal Cells

[0078] Medium formula: The N concentration of freshwater cyanobacteria medium BG11 is reduced to 1 / 4 of the original, which is 1 / 4N BG11 medium. Medium formula: NaNO 3 , 0.375g; K 2 HPO 4 , 0.04g; MgSO 4 .7H 2 O, 0.075g; CaCl 2 .7H 2 O, 0.036g; Na 2 CO 3 , 0.02g; citric acid, 0.006g; ferric citrate, 0.006g; trace element solution A5 1mL; dilute the above ingredients to 1000mL with distilled water.

[0079] Trace element solution A5: H 3 BO 4 , 2.86g; MnCl 2 .4H 2 O, 1.81g; ZnSO 4 , 0.222g; Na 2 MoO 4 , 0.39g; CuSO 4 .5H 2 O, 0.079g; Co(NO 3 ) 2 .6H 2 O, 49.4g.

[0080] The algal cell culture temperature is 30°C, and the light intensity is 2000lux.

[0081] Effect of 2.1 / 4N concentration on the growth of algal strains

[0082] Configure 1 / 4N concentration and norma...

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Abstract

The invention relates to a separation purifying method of a scenedesmus obliquus strain SoHNCJ2. The separation purifying method includes: diluting a sampled water sample, evenly coating a BG11 culture medium flat plate of 1.5-2% agar with the diluted water sample, and placing into a cultivation box for continuous illumination cultivation; using a toothpick to pick a single colony after the single colony grows on the flat plate, and cultivating the single colony on the BG11 flat plate by drawing a short streak; using an inoculation needle to perform separation and sterilizing on cultivated algae species by drawing a Z-shaped streak, and using a dilution and flat plate coating method to further purify the algae species; picking the single colony to draw a short streak, picking the short-streak colony to draw a Z-shaped streak, and alternating the process for 2-4 times to obtain the purified single colony. A cultivation method of the scenedesmus obliquus strain SoHNCJ2 is characterized in that the concentration of N or P of the fresh water cyanobacteria culture medium BG11 is lowered to 1 / 4 of the original concentration or increased to 3 times of the original concentration. The cultivated scenedesmus obliquus strain can grow continuously and fast under a low-concentration N source condition, algae cells can effectively accumulate a large amount of grease, and grease conversion production can be performed to produce biodiesel.

Description

technical field [0001] The invention belongs to the field of microbes and bioenergy, and in particular relates to a Scenedesmus obliquus algae strain and methods for separating, purifying, cultivating, collecting and using the same. Background technique [0002] With the increasing prosperity of social economy and the depletion of human available resources, for the sustainable development of society, governments and relevant technical personnel of various countries in the world are gradually turning their research direction to renewable energy. [0003] Because the algal resources on the earth are rich in species, many of which are rich in oil. Therefore, extracting oil from oil algae for the production of biodiesel to replace the depleted oil source has become a topic of great concern to the scientific community. In the prior art, the cultivation and extraction method of oil-rich algae and its related applications have been disclosed, but for its rich application, it is on...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/02C12P7/64A23K20/158C02F3/32C12R1/89
CPCC02F3/322C12N1/02C12N1/12C12P7/649C12N1/125C12R2001/89Y02E50/10
Inventor 王赟柯飞王红强屈二军梁峰杨雨彬
Owner HENAN UNIV OF URBAN CONSTR
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