Method for testing biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus

A technology of Scenedesmus obliquus and biotoxicity, applied in the measurement of color/spectral characteristics, etc., can solve problems such as drug residues, and achieve the effects of less usage, good reproducibility and environmental friendliness

Inactive Publication Date: 2019-10-25
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since such drugs are difficult to be completely absorbed in animals, most of them are excreted into the environment thro

Method used

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  • Method for testing biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus
  • Method for testing biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus
  • Method for testing biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus

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Experimental program
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Embodiment

[0026] 8 kinds of sulfa antibiotic standard items tested in the present embodiment are all purchased from U.S. Cato Research Chemicals Inc, and the standard items of sulfa antibiotics are tested as follows:

[0027] (1) Make a standard solution according to the solubility of the standard sulfonamide antibiotics, and store the standard solution in a refrigerator at 4°C until use.

[0028] (2) Cultivate Scenedesmus obliquus to reach the logarithmic growth phase, and use it as the working algae liquid, the specific operation is as follows:

[0029] The formula of medium stock solution is (mg·L -1 , usually this is the case unless otherwise specified): NaNO 3 1500, MgSO 4 ·7H 2 O 75,K 2 HPO 4 40, CaCl 2 2H 2 O 36 , Na 2 CO 3 20, Citric acid 6, Ferric ammonium citrate 6, EDTANa 2 1, where A 5 The solution formula is: add 286mg H in distilled water 3 BO 3 , 186mg MnCl 2 4H 2 O, 22mgZnSO 4 ·7H 2 O, 8mg CuSO 4 ·5H 2 O, 39 mg Na 2 MoO 4 2H 2 O, 5mg Co(NO 3 ) 2 ·...

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Abstract

The invention discloses a method for testing the biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus. With the sulfonamide antibiotics as a research object, the scenedesmus obliquus as a test organism and a 96-hole microplate as a carrier, through a 96-hole microplate sampling analysis method, an absorbance value of the scenedesmus obliquus at 681nm is tested by using a multi-function microplate reader after sampling for 96h, the growth inhibition ratio of the scenedesmus obliquus is calculated by using absorbance values of an experimental group and a control group, anegative logarithm pEC50 of an EC50 value is fitted and calculated by using a Matlab-based self-programmed Logit function, and the magnitude of the toxicity of the sulfonamide antibiotics is judged with the pEC50 value as a toxicity indicator. The method has the advantages of simple operation, convenience and quickness and good repeatability and can be widely applied to test the toxicity of the sulfonamide antibiotics.

Description

technical field [0001] The invention belongs to the field of biotoxicity detection of sulfonamide antibiotics, in particular to a method for testing the biotoxicity of sulfonamide antibiotics by using Scenedesmus obliques. Background technique [0002] Antibiotics are a class of chemical substances that can inhibit or affect biological functions at low concentrations, and have played a huge role in medical treatment and animal and plant pest control. Antibiotics can be divided into human antibiotics and veterinary antibiotics according to the objects of use. Sulfa drugs are a class of synthetic antibacterial agents. Since sulfa drugs were successfully synthesized in the 1940s, more than 150 sulfa drugs and their derivatives It is used in the treatment of human diseases or in animal feeding as veterinary medicine. Because such drugs are difficult to be completely absorbed in animals, most of them are excreted into the environment through feces and urine in the form of parent...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 钱丽梁延鹏覃礼堂莫凌云曾鸿鹄宋晓红
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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