Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for testing biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus

A technology of Scenedesmus obliquus and biotoxicity, applied in the measurement of color/spectral characteristics, etc., can solve problems such as drug residues, and achieve the effects of less usage, good reproducibility and environmental friendliness

Inactive Publication Date: 2019-10-25
GUILIN UNIVERSITY OF TECHNOLOGY
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since such drugs are difficult to be completely absorbed in animals, most of them are excreted into the environment through feces and urine in the form of parent or metabolites, resulting in a large amount of drug residues in the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for testing biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus
  • Method for testing biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus
  • Method for testing biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] 8 kinds of sulfa antibiotic standard items tested in the present embodiment are all purchased from U.S. Cato Research Chemicals Inc, and the standard items of sulfa antibiotics are tested as follows:

[0027] (1) Make a standard solution according to the solubility of the standard sulfonamide antibiotics, and store the standard solution in a refrigerator at 4°C until use.

[0028] (2) Cultivate Scenedesmus obliquus to reach the logarithmic growth phase, and use it as the working algae liquid, the specific operation is as follows:

[0029] The formula of medium stock solution is (mg·L -1 , usually this is the case unless otherwise specified): NaNO 3 1500, MgSO 4 ·7H 2 O 75,K 2 HPO 4 40, CaCl 2 2H 2 O 36 , Na 2 CO 3 20, Citric acid 6, Ferric ammonium citrate 6, EDTANa 2 1, where A 5 The solution formula is: add 286mg H in distilled water 3 BO 3 , 186mg MnCl 2 4H 2 O, 22mgZnSO 4 ·7H 2 O, 8mg CuSO 4 ·5H 2 O, 39 mg Na 2 MoO 4 2H 2 O, 5mg Co(NO 3 ) 2 ·...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for testing the biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus. With the sulfonamide antibiotics as a research object, the scenedesmus obliquus as a test organism and a 96-hole microplate as a carrier, through a 96-hole microplate sampling analysis method, an absorbance value of the scenedesmus obliquus at 681nm is tested by using a multi-function microplate reader after sampling for 96h, the growth inhibition ratio of the scenedesmus obliquus is calculated by using absorbance values of an experimental group and a control group, anegative logarithm pEC50 of an EC50 value is fitted and calculated by using a Matlab-based self-programmed Logit function, and the magnitude of the toxicity of the sulfonamide antibiotics is judged with the pEC50 value as a toxicity indicator. The method has the advantages of simple operation, convenience and quickness and good repeatability and can be widely applied to test the toxicity of the sulfonamide antibiotics.

Description

technical field [0001] The invention belongs to the field of biotoxicity detection of sulfonamide antibiotics, in particular to a method for testing the biotoxicity of sulfonamide antibiotics by using Scenedesmus obliques. Background technique [0002] Antibiotics are a class of chemical substances that can inhibit or affect biological functions at low concentrations, and have played a huge role in medical treatment and animal and plant pest control. Antibiotics can be divided into human antibiotics and veterinary antibiotics according to the objects of use. Sulfa drugs are a class of synthetic antibacterial agents. Since sulfa drugs were successfully synthesized in the 1940s, more than 150 sulfa drugs and their derivatives It is used in the treatment of human diseases or in animal feeding as veterinary medicine. Because such drugs are difficult to be completely absorbed in animals, most of them are excreted into the environment through feces and urine in the form of parent...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 钱丽梁延鹏覃礼堂莫凌云曾鸿鹄宋晓红
Owner GUILIN UNIVERSITY OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com