Method for fluorescence detection of biotoxicity of desmetryn by scenedesmus obliquus in ammonium salt environment

A technology of Scenedesmus obliquus and biotoxicity, which is applied in the direction of fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems of unstable luminescent bacteria and high method cost of the luminescent bacteria method, and achieve intuitive biological toxicity, Effect of promoting action and improving detection sensitivity

Active Publication Date: 2017-05-31
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

At present, the luminescent bacteria method is a relatively mature biological toxicity detection method. The toxic effect of diquat on luminescent bacteria is reflected by the change of the luminous intensity of the luminescent bacteria, so as to determine the toxicity of diquat. However, the luminescent bacteria method also has the instability of luminescent bacteria. and the high cost of the method, so it is urgent to develop a stable and low-cost method for determining the biotoxicity of diquat

Method used

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  • Method for fluorescence detection of biotoxicity of desmetryn by scenedesmus obliquus in ammonium salt environment
  • Method for fluorescence detection of biotoxicity of desmetryn by scenedesmus obliquus in ammonium salt environment
  • Method for fluorescence detection of biotoxicity of desmetryn by scenedesmus obliquus in ammonium salt environment

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preparation example Construction

[0044] 3. Preparation of Scenedesmus obliquus liquid

[0045] The preparation of Scenedesmus obliquus algae liquid comprises, the logarithmic phase Scenedesmus obliquus obtained in the cultivation process of the above-mentioned 2 Scenedesmus obliquus species, the algae cell density is obtained by microscopic inspection, and the selected cell density ranges from (1-2)×10 6 ind / ·ml -1 The logarithmic phase Scenedesmus obliquus algae liquid, used for the determination of the biological toxicity of diquat.

[0046] 4. Preparation of Diquat Standard Solution

[0047] 10mg / L diquat stock solution: take 10μl of diquat stock solution with a concentration of 200g / L, add pure water to dilute to 200ml, and obtain diquat stock solution;

[0048] 200μg / L Diquat intermediate solution: Take 2.0ml of Diquat stock solution in a 100ml volumetric flask, dilute with pure water to obtain Diquat intermediate solution;

[0049] Diquat standard solution: take 2.5, 5.0, 7.5, 10.0, 12.5ml of diquat...

Embodiment 1

[0051] Determination of Effect of Diquat on Fluorescent Effect of Scenedesmus obliquus

[0052] In the logarithmic growth phase of Scenedesmus obliquus (i.e. cultured for 5-6 days), take Scenedesmus obliquus algae liquid, diquat standard solution of different concentrations and distilled water in a volume ratio of 1:1:1, mix and shake well, and At the same time, a blank group (distilled water instead of diquat) was set as a control. After the mixture was placed in the dark for 15 minutes, the kinetic parameters of chlorophyll fluorescence in Scenedesmus obliquus were measured with a water sample chlorophyll fluorescence meter, including the maximum light energy conversion efficiency (Fv / Fm) of photosystem II, the actual light energy of photosystem II Transformation efficiency (Y(II)), photosynthetic electron transfer efficiency (ETR), photochemical quenching (qP) and non-photochemical quenching (NPQ), three parallel control experiments were carried out, and the average value w...

Embodiment 2

[0056] Determination of Effect of Diquat on Fluorescent Effect of Scenedesmus obliquus

[0057] In the logarithmic phase of the growth of Scenedesmus obliquus (i.e. cultured for 5-6 days), take Scenedesmus oblique algae liquid, diquat standard solution of different concentrations and distilled water in a volume ratio of 2:1:2, mix and shake well, and At the same time, a blank group (distilled water instead of diquat) was set as a control. After the mixture was placed in the dark for 10 min, the kinetic parameters of chlorophyll fluorescence in Scenedesmus obliquus were measured with a water sample chlorophyll fluorescence meter, including the maximum light energy conversion efficiency (Fv / Fm) of photosystem II, the actual light energy of photosystem II Transformation efficiency (Y(II)), photosynthetic electron transfer efficiency (ETR), photochemical quenching (qP) and non-photochemical quenching (NPQ), three parallel control experiments were carried out, and the average value...

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Abstract

The invention provides a method for fluorescence detection of biotoxicity of desmetryn by scenedesmus obliquus in ammonium salt environment, and belongs to the technical field of scenedesmus obliquus. The method comprises the steps of scenedesmus obliquus seed culture and scenedesmus obliquus liquid preparation, and also comprises influence determination of the desmetryn on the scenedesmus obliquus fluorescence effect in the ammonium salt environment. According to the method, the series concentration of desmetryn standard solutions added with ammonium salt solution are taken and are added into the scenedesmus obliquus solution; sufficient mixing and uniform shaking are performed; the volume ratio of the ammonium salt solution to the desmetryn standard solution to the scenedesmus obliquus liquid is controlled to be 2:(1 to 2):(1 to 2); meanwhile, distilled water is used for replacing a blank control group of the scenedesmus obliquus standard solution; after the dark placement for 10 to 15min, a water sample fluorescence detection instrument is used for determining each chlorophyll fluorescence dynamic parameter of the scenedesmus obliquus. The method provided by the invention has the advantages that the method is simple; the operation is easy; the biotoxicity of the desmetryn can be directly reflected.

Description

technical field [0001] The invention belongs to the technical field of Scenedesmus obliquus, and relates to measuring the biological toxicity of diquat by using indicator algae, in particular to a method for detecting the biological toxicity of diquat by using Scenedesmus obliquus fluorescence under an ammonium salt environment. The method of the invention is fast and sensitive. Background technique [0002] Diquat English name DesMetry, molecular formula C 8 h 15 N 5 S, affects the photosynthesis of weeds, destroys the cell membrane, and the weeding effect is that the tips, edges and veins of the leaves turn yellow and dry, and finally achieve the purpose of weeding. [0003] Diquat is better for cotton, soybean, wheat, peanut, sunflower, potato, fruit tree, vegetable, tea tree and paddy fields to control barnyardgrass, crabgrass, daughter, wild amaranth, Polygonum, Chenopodium, purslane, see Annual grasses and broad-leaved grasses such as Mai Niang, Chickweed, and Plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 崔建升赵德华
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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