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Open-type method for producing microalgae oil

An open technology of microalgae oil, applied in the field of biotechnology and bioenergy, can solve the problems of the influence of algae growth and reproduction, no oil content involved, culture failure, etc., to help the accumulation of cell oil and improve the efficiency of carbon fixation and oil content, and the effect of inhibiting bacterial contamination

Active Publication Date: 2019-06-14
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
However, open culture is easily polluted by harmful organisms such as filamentous fungi, rotifers, and protozoa, which grow and reproduce in algae culture fluid.
When the number of these harmful organisms reaches a certain density, it will affect the growth and reproduction of the cultured algae. The light ones of the polluted algae liquid are not conducive to further expansion of the culture, and the severe ones will lead to the failure of the culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) In a 12L open photobioreactor, add Pseudochlorella kescheri FSH-Y3 seed solution and microalgae medium, the amount of seed solution added is 400mL, and the pH value of the microalgae medium is adjusted to 10, The amount of addition is 8L, the light intensity of the culture is 5000Lux, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced. 2 The content of NO is 5v%, and the content of NO is 50×10 -6 (v / v).

[0032] (2) After 4 days of cultivation, insert 400 mL of monostipid SHJ-02 seed liquid and 400 mL of Chlorella SF-B1 seed liquid, adjust the pH of the microalgae culture system to 8, and the temperature to 10 ° C, continuous light culture, light The intensity is 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v).

[0033] (3) After 7 days of culture, enter the stable growth period, end the culture, harvest the microalgae ...

Embodiment 2

[0035] (1) In a 12L open photobioreactor, add Pseudochlorella kescheri FSH-Y3 seed solution and microalgae medium, the amount of seed solution added is 800mL, and the pH value of the microalgae medium is adjusted to 12, The amount of addition is 8L, the light intensity of the culture is 5000Lux, the culture temperature is 20°C, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced. 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v).

[0036] (2) After culturing for 2 days, insert 560 mL of the seed liquid of Monospina SHJ-02 and 560 mL of the seed liquid of Chlorella SF-B1, adjust the pH of the microalgae culture system to 10, and culture at 5°C under continuous light. The light intensity is 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v).

[0037] (3) After cultivating for 8 days, it enters the stationary phase, ends the culturing, harvests the microa...

Embodiment 3

[0039] (1) In 12 open-type photobioreactors, add Kjeldahl FSH-Y3 seed liquid and microalgae medium, the amount of seed liquid added is 800mL, the pH value of microalgae medium is adjusted to 11, add The volume is 8L, the light intensity of the culture is 5000Lux, the culture temperature is 30°C, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v).

[0040] (2) After 2 days of cultivation, insert 280mL of monostipium SHJ-02 seed solution and 280mL of chlorella SF-B1 seed solution, adjust the pH of the microalgae culture system to 9, culture temperature at 15°C, continuous light culture, light The intensity is 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v).

[0041] (3) After cultivating for 8 days, it enters the stationary phase, ends the culturing, harvests the microalgae cells by centrifugation, and measures the...

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Abstract

The invention relates to an open-type method for producing microalgae oil. The method comprises the following steps: (1) a microalgae culture medium and parachlorella kessleri FSH-Y3 or / and a scenedesmus obliquus FSH-Y2 seed solution are added to an open reaction tank, the pH is adjusted to 10 to 12, the temperature is 20 to 30 DEG C, and gas having a volume of CO 2 being 1 v% to 5 v% is introduced for culturing for a certain period of time; and (2) the pH of a culture system is adjusted to 8 to 10, the temperature is 0 to 20 DEG C, and a chlorella SF-B1 seed solution and a single needle algaeSHJ-02 seed solution are inoculated, the gas with a volume of CO2 of 5v% to 45v% is introduced, and culturing is carried out under light conditions to obtain the microalgae cells. The method of the invention improves the tolerance and solubility of the microalgae culture system to high concentration of CO2, improves the carbon fixation efficiency and the oil yield, and can inhibit the contamination of the bacteria during the cultivation process.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to an open method for producing microalgae oil. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/12C12R1/89
CPCY02P20/59
Inventor 师文静李晓姝王鹏翔孙启梅张霖樊亚超
Owner CHINA PETROLEUM & CHEM CORP
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