A kind of Scenedesmus obliquus tolerant to high pH and its breeding method

A technology of Scenedesmus obliquus and tolerance, which is applied in the field of microbial engineering, can solve the problems of low solubility and low carbon dioxide efficiency, and achieve the effects of increasing growth rate, improving fixation efficiency, and increasing fixation

Active Publication Date: 2017-05-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scenedesmus obliquus selected and bred by the present invention can better absorb and utilize carbon dioxide under high pH value, and grow and reproduce rapidly, which solves the problem that the existing Scenedesmus obliquus can only grow under neutral pH, while under neutral pH condition There are problems of low solubility of carbon dioxide and low efficiency of fixing carbon dioxide

Method used

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  • A kind of Scenedesmus obliquus tolerant to high pH and its breeding method
  • A kind of Scenedesmus obliquus tolerant to high pH and its breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Isolation and Screening of Mutants

[0038] The original Scenedesmus obliquus strain of the present invention is a wild Scenedesmus obliquus strain isolated from a water sample of Hunhe River in Fushun City, Liaoning Province by means of plate marking in October 2011, and the number is FSH-Y1. The original Scenedesmus obliquus strain FSH-Y1 was subjected to ultraviolet mutagenesis and domesticated culture to select a mutant strain of Scenedesmus obliquus that tolerated high pH.

[0039] Specific steps are as follows:

[0040] (1) Prepare BG11 medium, divide the prepared BG11 medium into 250mL shake flasks, each bottle has a liquid volume of 100mL, seal it with a gas-permeable film, wrap it in kraft paper, and sterilize it at 121°C and 0.1MPa for 30 minute.

[0041] (2) Under aseptic conditions, draw about 10 mL of Scenedesmus obliquus liquid FSH-Y1 and inoculate it into the above-mentioned sterilized shake flask, and place the shake flask in a constant tempe...

Embodiment 2

[0048] Comparison of the growth rate of the original algae strain and the mutant algae strain at different pH: the algae liquid in the logarithmic growth phase of the screened algae strain FSH-Y2 was inoculated with the algae liquid in the logarithmic growth phase of the original algae species in a certain proportion. into shake flasks filled with BG11 medium, the initial OD after inoculation 680 The value is the same, both are 0.2, cultivated for 15 days under different pH values, and measure the OD 680 The value is used as an index to measure the growth rate, and the results are shown in Table 3.

[0049] Table 3 Comparison of OD values ​​between original algal strains and mutagenic algal strains at different pH values

[0050]

[0051] It can be seen from Table 3 that the OD value of the mutagenized algal strains did not change significantly with the increase of pH value, while the OD value of the original algal strains decreased rapidly, grew slowly, and even died.

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Abstract

The invention provides a Scenedesmus obliquus tolerant to high pH and a breeding method thereof. The present invention selects Scenedesmus obliqnus through ultraviolet mutagenesis and step-by-step PH domestication and screening methods, and obtains a mutant strain of Scenedesmus obliqnus (Scenedesmus obliqnus) that is tolerant to high pH, ​​named FSH-Y2, and was produced in September 2012 On the 11th, it was deposited in the General Microorganism Collection Center of the China Microbiological Culture Collection Management Committee, and the preservation number was CGMCC No. 6551. Scenedesmus obliques selected by the present invention can better absorb and utilize carbon dioxide under high pH value, and grow and reproduce rapidly, which solves the problem that the existing Scenedesmus obliques can only grow under neutral pH, and exists under neutral pH conditions. The solubility of carbon dioxide is small and the efficiency of fixing carbon dioxide is low.

Description

technical field [0001] The invention belongs to the field of microbial engineering, and in particular relates to a Scenedesmus obliquus tolerant to high pH and a breeding method thereof. Background technique [0002] With the shortage of resources in human society and the increasingly prominent environmental problems, the whole world is facing the crisis of energy shortage and ecological environment damage. Therefore, seeking a green and renewable new energy has become a scientific issue that scientists all over the world are generally concerned about. Among many organisms, microalgae have the characteristics of rich resources, various species, high photosynthetic utilization efficiency, fast growth, strong adaptability, and high content of self-synthesized oil, and have attracted the attention of researchers from various countries in recent years. [0003] Scenedesmus, also known as Scenedesmus, is a genus of the Scenedesmus family of the order Chlorophyta Chlorophyta. It i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N13/00C12R1/89
CPCC12N13/00C12N15/01C12N1/125C12R2001/89
Inventor 师文静高大成廖莎金平王领民李晓姝张霖王崇辉姚新武
Owner CHINA PETROLEUM & CHEM CORP
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