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Method for evaluating toxicity of single triazole bactericide agricultural chemicals by using mixed green alga

A technology of triazoles and fungicides, which is applied in the field of environmental toxicology to achieve the effects of high accuracy, fast speed and strong practicability

Inactive Publication Date: 2019-09-17
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] At present, a single algae species under pure culture conditions has been widely used in the research on the safety evaluation of pesticide acute toxicity. There are few reports on the safety evaluation of pesticide acute toxicity with various algae species under different conditions

Method used

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  • Method for evaluating toxicity of single triazole bactericide agricultural chemicals by using mixed green alga
  • Method for evaluating toxicity of single triazole bactericide agricultural chemicals by using mixed green alga
  • Method for evaluating toxicity of single triazole bactericide agricultural chemicals by using mixed green alga

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Experimental program
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Embodiment

[0025] 1.1 Preparation of related drugs

[0026] 1.1.1 Configuration of the mother liquor of the test substance pesticide triazole fungicide: weigh a certain amount of the original drug, put it into a small beaker and add ultrapure water to dissolve it to a constant volume, and add volume concentration to the insoluble or slightly soluble triazole fungicide Dimethyl sulfoxide controlled within 5‰, and ultrasonically assisted to dissolve, add water to volume after dissolving.

[0027] 1.1.2 Preparation of relevant concentrations for toxicity experiments: Dilute the mother liquor with ultrapure water and prepare test solutions with gradient concentrations.

[0028] 1.1.3 Preparation of BG11 medium: BG11 medium was used for the pure medium and mixed medium of the three types of green algae, and its formula is shown in Table 1. Prepare the BG11 medium formula according to the formula in Table 1. The preparation method is as follows: prepare the ingredients in Table 1 to make the ...

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Abstract

The invention discloses a method for evaluating toxicity of single triazole bactericide agricultural chemicals by using mixed green alga. Three kinds of algae (mixed green alga) of Selenastrumcapricornutum (Selenastrumcapricornutum), Scenedesmus obliquus ( Scenedesmus obliquus ) and Chlorellapyrenoidosa (Chlorellapyrenoidosa) are subjected to mixed culture, to evaluate the single toxicity of three typical agricultural chemical triazole bactericides (triazolone, myclobutanil, tebuconazole ). Under the action of the triazole bactericide agricultural chemicals, for the half effective concentration EC50 of the mixed green alga, the single toxicity of the triazole bactericide agricultural chemicals is evaluated. The method considers the monopoly of single algae kinds in a natural water body, and varied algae are in intergrowth, so that a mixed culture method closed to the environment of the natural water body is adopted to objectively and comprehensively evaluate the potential threaten of pollutants. The method also has the advantages of being simple and quick to test, saving consumption of expensive chemicals, and the like toxotest is convenient to repeatedly and parallelly performed, so that the test results are accurate.

Description

technical field [0001] The invention belongs to the field of environmental toxicology, in particular to a method for evaluating the toxicity of a single triazole fungicide pesticide by using mixed green algae. In this method, triadimefon (SZT), myclobutanil (JJZ) Single toxicity of triazole fungicide pollutants of three typical pesticides triazole fungicides (WZC). Background technique [0002] Triazole fungicides have become one of the most effective and promising fungicides because of their antibacterial and plant growth regulating effects. At present, it has been widely used in the prevention and control of pests and diseases of vegetables, fruits and flowers. Its extensive use will inevitably enter water bodies with rainfall and surface runoff, which may cause harm to aquatic organisms, which may affect the entire ecosystem. [0003] Algae are primary producers of aquatic ecosystems, play an extremely important role in the balance and stability of water bodies, and ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/89
CPCC12Q1/18G01N2333/405
Inventor 覃礼堂农琼媛莫凌云代俊峰曾鸿鹄梁延鹏
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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