Method for determining toxic effect of scenedesmus obliquus by copper ions in different fresh water environments, and application

A technology for Scenedesmus obliquus and determination methods, which is applied in the direction of testing water, measuring devices, instruments, etc., and can solve the problems of scarcity and poor adaptability of algae toxicity assessment methods

Inactive Publication Date: 2019-04-05
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the scarcity and poor adaptability of existing methods for assessing the toxicity of heavy metals to algae in freshwater environments, the present invention provides a method for predicting the toxicity of copper ions to Scenedesmus obliquus in freshwater environments and its application

Method used

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  • Method for determining toxic effect of scenedesmus obliquus by copper ions in different fresh water environments, and application
  • Method for determining toxic effect of scenedesmus obliquus by copper ions in different fresh water environments, and application
  • Method for determining toxic effect of scenedesmus obliquus by copper ions in different fresh water environments, and application

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Embodiment 1

[0084] The present invention carried out a total of 32 groups of copper on the 72h acute toxicity experiment of Scenedesmus obliquus (each group had 6 copper concentration levels, and the copper gradient range covered the semi-inhibiting copper concentration value), and performed different single factors (Ca, The changes of Mg, Na, K, pH) aim to quantify the acute toxic effects of copper on Scenedesmus obliquus under different water chemical characteristics. The species of Scenedesmus obliquus was purchased from Wuhan Aquatic Biology, Chinese Academy of Sciences. Based on BG11 medium, plus CaCl 2 , MgSO 4 , NaCl, KCl, HCl, NaOH, and biological buffer MOPS and using the controlled variable method to perform acute toxicity tests on Scenedesmus obliquus while only changing certain experimental conditions while controlling other experimental conditions unchanged. The algae preculture was carried out in a 3L Erlenmeyer flask, using BG11 medium. The acute toxicity test was carried o...

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Abstract

The invention provides a method for determining a toxic effect of scenedesmus obliquus by copper ions in different fresh water environments, and an application. The morphology and concentration of copper, competitive positive ions and the influence of pH on the toxicity of the scenedesmus obliquus are comprehensively considered; and an obtained toxicity prediction model is verified in an experiment and has high accuracy in weak alkaline fresh water with the pH value higher than 7.

Description

(1) Technical field [0001] The invention belongs to the field of ecological heavy metal toxicity prediction. More specifically, it relates to a method and application for determining the toxic effect of copper ions on Scenedesmus obliquus in different freshwater environments. It is a method that takes into account the coordination of cations and heavy metal ions in the biofilm. The method of calculating biological toxicity based on physical competition, cationic form, dissolved organic matter, temperature, hardness, alkalinity and pH value. (2) Background technology [0002] Copper is an essential element of all animals and plants. It exists in all organs and tissues of living organisms. It can usually be combined with protein and some organic matter. Many key enzymes in the metabolism of organisms require the participation and activation of copper in order to maintain their normal physiological functions. On the other hand, excessive copper intake will produce copper poisoning,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
CPCG01N33/1813
Inventor 周瑛徐潇李若嘉
Owner ZHEJIANG UNIV OF TECH
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