Beauty and skin care enzyme and preparation method thereof

Abstract

The invention relates to a beauty and skin care enzyme and a preparation method thereof. According to the invention, fruits and traditional Chinese medicines are used as main fermentation raw materials, the fruits and the traditional Chinese medicines are crushed and mixed with fermentation strains, and active substances in each raw material are effectively retained through two-stage fermentation, filtration separation and freeze-drying treatment, SOD and sea buckthorn flavone contained in sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis, and the sea buckthorn fruits have the skin care effects of resisting oxidation and promoting collagen synthesis. Salidroside contained in the rhodiola rosea has the skin care effects of resisting light aging and fatigue, astragalus polysaccharide and astragaloside contained in the astragalus membranaceus can repair a barrier and enhance immunity, ophiopogonin and polysaccharide contained in the radix ophiopogonis have the effects of preserving moisture and resisting saccharification, and hesperidin and nobiletin contained in the pericarpium citri reticulatae have the effects of resisting oxidation and adjusting grease secretion. The obtained compound enzyme has the effects of resisting oxidation, resisting inflammation and preserving moisture, has activity, stability and transdermal efficiency, and can be used in skin care products such as anti-oxidation essence, repair masks, late-up cream and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of enzyme preparation, in particular to a beauty and skin care enzyme and a preparation method thereof. Background Art

[0002] Enzymes, also known as "enzymes," are proteins smaller than a cell unit and specialized proteins with biocatalytic properties. They mediate various biochemical changes in the human body's metabolism and are essential for cell metabolism, regeneration, decomposition, digestion, and more. Enzymes are essential for life; their absence signifies impending death. Enzyme activity varies with age. From birth to early childhood, enzyme activity increases and continues to improve. After middle age, enzyme activity declines due to lifestyle factors and environmental pollution, and metabolic function continues to decline with age.

[0003] Plant enzymes, produced through microbial fermentation, are rich in enzymes, proteins, vitamins, and active substances with a wide range of physiological benefits. They can inhibit skin aging, repair wrinkles, and increase skin radiance and elasticity. Currently, there are many skincare products containing enzymes, but most enzymes easily lose their activity in these products. Larger enzyme molecules have difficulty penetrating the stratum corneum, resulting in poor results. Their single purpose of exfoliation or moisturizing lacks consistent efficacy and has low application value. Summary of the Invention

[0004] In view of the problems existing in the prior art, the present invention discloses a beauty and skin care enzyme, the main raw materials and weight ratios used in its preparation are:

[0005] A method for preparing a beauty and skin care enzyme, characterized in that it comprises the following steps: Step 1: fresh seabuckthorn fruits are selected, seeds are removed, and pulped, and pectinase is inactivated by pasteurization to obtain seabuckthorn pulp; Step 2: Selecting Rhodiola rosea and Astragalus membranaceus, respectively extracting and concentrating them using a water extraction process to obtain a Rhodiola rosea extract and an Astragalus membranaceus concentrate with a soluble solid content of not less than 20%; Step 3, selecting Radix Ophiopogonis and Pericarpium Citri Reticulatae, and freeze-drying them and then ultrafine grinding them, and sieving them with a sieve to obtain freeze-dried Radix Ophiopogonis powder and ultrafine Pericarpium Citri Reticulatae powder; Step 4: selecting sea buckthorn fruit, rhodiola rosea extract, astragalus concentrate, lily dextrose freeze-dried powder, and tangerine peel ultrafine powder according to weight ratio, mixing them evenly, and then adding 3% inoculum of plant lactobacillus, 1% suspension of Aspergillus niger spores, and 0.5% inoculum of saccharomyces cerevisiae to obtain a pre-fermentation mixture; Step 5, the pre-fermentation mixture is fermented in stages, the aerobic stage is controlled at 25°C, the rotation speed is 150 rpm, and the fermentation time is 22-26 hours; the anaerobic stage is controlled at 20°C for static fermentation, and the fermentation time is 70-75 hours. When the amylase activity is not less than 250 U / g and the total phenol content is not less than 8 mg / g, the fermentation is terminated to obtain a fermentation liquid; Step 6: The fermentation broth was centrifuged at 8000 rpm for 15 minutes, the supernatant was sterilized by microfiltration using a 0.22 μm filter membrane, and then concentrated by ultrafiltration to obtain a filtrate; Step 7: Add trehalose to the filtrate in proportion, mix well, and then perform vacuum freeze-drying to obtain enzyme powder.

[0006] As a preferred embodiment of the present invention, the pasteurization temperature in step 1 is 60° C. and the time is 20 minutes.

[0007] As a preferred embodiment of the present invention, the water extraction process of Rhodiola rosea in step 2 is to add Rhodiola rosea to 60°C pure water according to the ratio of 1KG Rhodiola to 5L pure water, extract for 90 minutes, and perform low-temperature concentration under the conditions of a vacuum degree of -0.08Mpa and a temperature not exceeding 60°C; the water extraction process of Astragalus membranaceus is to add Astragalus membranaceus to 60°C pure water according to the ratio of 1KG Astragalus membranaceus to 8L pure water, extract for 120 minutes, and perform reduced pressure concentration in a 60°C water bath under the condition of a vacuum degree of -0.06Mpa.

[0008] As a preferred embodiment of the present invention, the sieve used in step 3 is 300 mesh.

[0009] As a preferred embodiment of the present invention, a 10 kDa membrane is used for the ultrafiltration concentration in step six.

[0010] As a preferred embodiment of the present invention, the temperature of the vacuum freeze-drying in step seven is -50°C.

[0011] The beneficial effects of the present invention are as follows: the present invention uses fruits and traditional Chinese medicine as main fermentation raw materials, and after crushing, mixes with fermentation bacteria, undergoes two-stage fermentation, and is filtered, separated, and freeze-dried to effectively retain the active substances in each raw material. Among them, the SOD and seabuckthorn flavonoids contained in seabuckthorn fruit have skin care effects of anti-oxidation and promoting collagen synthesis, the salidroside contained in rhodiola rosea has skin care effects of anti-photoaging and anti-fatigue, the astragalus polysaccharides and astragaloside IV contained in astragalus can repair the barrier and enhance immunity, the ophiopogon saponins and polysaccharides contained in ophiopogon have moisturizing and anti-glycation effects, and the hesperidin and nobiletin contained in tangerine peel have antioxidant and oil secretion regulating effects. The obtained composite enzyme has antioxidant, anti-inflammatory, and moisturizing effects, takes into account activity, stability, and transdermal efficiency, and can be used in skin care products such as antioxidant essences, repair masks, and night creams. DETAILED DESCRIPTION

[0012] Example 1

[0013] The main raw materials and weight ratios of the beauty and skin care enzymes used in the preparation of the present invention are as follows:

[0014] A method for preparing a beauty and skin care enzyme, characterized in that it comprises the following steps: Step 1: fresh seabuckthorn fruit is selected, the seeds are removed, and the pulp is beaten, and the pectinase is inactivated by pasteurization at a temperature of 60° C. for 20 minutes to obtain seabuckthorn pulp; Step 2: Select Rhodiola rosea and Astragalus membranaceus, respectively, and extract and concentrate them by water extraction process to obtain Rhodiola rosea extract and Astragalus membranaceus concentrate with a soluble solid content of not less than 20%; wherein, the Rhodiola rosea water extraction process is to configure 5L of pure water according to 1KG of Rhodiola rosea, add Rhodiola rosea to 60°C pure water, extract for 90 minutes, and perform low-temperature concentration under the conditions of a vacuum degree of -0.08Mpa and a temperature of not more than 60°C; the Astragalus membranaceus water extraction process is to configure 8L of pure water according to 1KG of Astragalus membranaceus, add Astragalus membranaceus to 60°C pure water, extract for 120 minutes, and perform reduced pressure concentration in a 60°C water bath under the conditions of a vacuum degree of -0.06Mpa; Step 3: Select Radix Ophiopogonis and Pericarpium Citri Reticulatae, freeze-dry them and perform ultrafine grinding, and sieve them through a 300-mesh sieve to obtain freeze-dried Radix Ophiopogonis powder and ultrafine Pericarpium Citri Reticulatae powder; Step 4: selecting sea buckthorn fruit, rhodiola rosea extract, astragalus concentrate, lily dextrose freeze-dried powder, and tangerine peel ultrafine powder according to weight ratio, mixing them evenly, and then adding 3% inoculum of plant lactobacillus, 1% suspension of Aspergillus niger spores, and 0.5% inoculum of saccharomyces cerevisiae to obtain a pre-fermentation mixture; Step 5, the pre-fermentation mixture is fermented in stages, the aerobic stage is controlled at 25°C, the rotation speed is 150 rpm, and the fermentation time is 22 hours; the anaerobic stage is controlled at 20°C for static fermentation, and the fermentation time is 70 hours. When the amylase activity is not less than 250 U / g and the total phenol content is not less than 8 mg / g, the fermentation liquid is obtained; Step 6: The fermentation broth was centrifuged at 8000 rpm for 15 minutes, the supernatant was sterilized by microfiltration using a 0.22 μm filter membrane, and then ultrafiltration and concentration were performed through a 10 kDa membrane to obtain a filtrate; Step 7: Add trehalose to the filtrate in proportion, mix well, and then perform vacuum freeze-drying at -50°C to obtain enzyme powder.

[0015] Example 2

[0016] The main raw materials and weight ratios of the beauty and skin care enzymes used in the preparation of the present invention are as follows:

[0017] A method for preparing a beauty and skin care enzyme, characterized in that it comprises the following steps: Step 1: fresh seabuckthorn fruit is selected, the seeds are removed, and the pulp is beaten, and the pectinase is inactivated by pasteurization at a temperature of 60° C. for 20 minutes to obtain seabuckthorn pulp; Step 2: Select Rhodiola rosea and Astragalus membranaceus, respectively, and extract and concentrate them by water extraction process to obtain Rhodiola rosea extract and Astragalus membranaceus concentrate with a soluble solid content of not less than 20%; wherein, the Rhodiola rosea water extraction process is to configure 5L of pure water according to 1KG of Rhodiola rosea, add Rhodiola rosea to 60°C pure water, extract for 90 minutes, and perform low-temperature concentration under the conditions of a vacuum degree of -0.08Mpa and a temperature of not more than 60°C; the Astragalus membranaceus water extraction process is to configure 8L of pure water according to 1KG of Astragalus membranaceus, add Astragalus membranaceus to 60°C pure water, extract for 120 minutes, and perform reduced pressure concentration in a 60°C water bath under the conditions of a vacuum degree of -0.06Mpa; Step 3: Select Radix Ophiopogonis and Pericarpium Citri Reticulatae, freeze-dry them and perform ultrafine grinding, and sieve them through a 300-mesh sieve to obtain freeze-dried Radix Ophiopogonis powder and ultrafine Pericarpium Citri Reticulatae powder; Step 4: selecting sea buckthorn fruit, rhodiola rosea extract, astragalus concentrate, lily dextrose freeze-dried powder, and tangerine peel ultrafine powder according to weight ratio, mixing them evenly, and then adding 3% inoculum of plant lactobacillus, 1% suspension of Aspergillus niger spores, and 0.5% inoculum of saccharomyces cerevisiae to obtain a pre-fermentation mixture; Step 5, the pre-fermentation mixture is fermented in stages, the aerobic stage is controlled at 25°C, the rotation speed is 150 rpm, and the fermentation time is 26 hours; the anaerobic stage is controlled at 20°C for static fermentation, and the fermentation time is 75 hours. When the amylase activity is not less than 250 U / g and the total phenol content is not less than 8 mg / g, the fermentation liquid is obtained; Step 6: The fermentation broth was centrifuged at 8000 rpm for 15 minutes, the supernatant was sterilized by microfiltration using a 0.22 μm filter membrane, and then ultrafiltration and concentration were performed through a 10 kDa membrane to obtain a filtrate; Step 7: Add trehalose to the filtrate in proportion, mix well, and then perform vacuum freeze-drying at -50°C to obtain enzyme powder.

[0018] Example 3

[0019] The main raw materials and weight ratios of the beauty and skin care enzymes used in the preparation of the present invention are as follows:

[0020] A method for preparing a beauty and skin care enzyme, characterized in that it comprises the following steps: Step 1: fresh seabuckthorn fruit is selected, the seeds are removed, and the pulp is beaten, and the pectinase is inactivated by pasteurization at a temperature of 60° C. for 20 minutes to obtain seabuckthorn pulp; Step 2: Select Rhodiola rosea and Astragalus membranaceus, respectively, and extract and concentrate them by water extraction process to obtain Rhodiola rosea extract and Astragalus membranaceus concentrate with a soluble solid content of not less than 20%; wherein, the Rhodiola rosea water extraction process is to configure 5L of pure water according to 1KG of Rhodiola rosea, add Rhodiola rosea to 60°C pure water, extract for 90 minutes, and perform low-temperature concentration under the conditions of a vacuum degree of -0.08Mpa and a temperature of not more than 60°C; the Astragalus membranaceus water extraction process is to configure 8L of pure water according to 1KG of Astragalus membranaceus, add Astragalus membranaceus to 60°C pure water, extract for 120 minutes, and perform reduced pressure concentration in a 60°C water bath under the conditions of a vacuum degree of -0.06Mpa; Step 3: Select Radix Ophiopogonis and Pericarpium Citri Reticulatae, freeze-dry them and perform ultrafine grinding, and sieve them through a 300-mesh sieve to obtain freeze-dried Radix Ophiopogonis powder and ultrafine Pericarpium Citri Reticulatae powder; Step 4: selecting sea buckthorn fruit, rhodiola rosea extract, astragalus concentrate, lily dextrose freeze-dried powder, and tangerine peel ultrafine powder according to weight ratio, mixing them evenly, and then adding 3% inoculum of plant lactobacillus, 1% suspension of Aspergillus niger spores, and 0.5% inoculum of saccharomyces cerevisiae to obtain a pre-fermentation mixture; Step 5, the pre-fermentation mixture is fermented in stages, the aerobic stage is controlled at 25°C, the rotation speed is 150 rpm, and the fermentation time is 24 hours; the anaerobic stage is controlled at 20°C for static fermentation, and the fermentation time is 72 hours. When the amylase activity is not less than 250 U / g and the total phenol content is not less than 8 mg / g, the fermentation liquid is obtained; Step 6: The fermentation broth was centrifuged at 8000 rpm for 15 minutes, the supernatant was sterilized by microfiltration using a 0.22 μm filter membrane, and then ultrafiltration and concentration were performed through a 10 kDa membrane to obtain a filtrate; Step 7: Add trehalose to the filtrate in proportion, mix well, and then perform vacuum freeze-drying at -50°C to obtain enzyme powder.

[0021] Components not described in detail herein are prior art.

[0022] Although the specific embodiments of the present invention have been described in detail above, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by ordinary technicians in this field, various changes can be made without departing from the purpose of the present invention. Modifications or deformations that do not involve creative work are still within the scope of protection of the present invention.

Claims

1. A beauty and skin care enzyme, characterized in that: The main raw materials and weight proportions for its preparation are: 40-50 parts of sea buckthorn pulp, 15-20 parts of rhodiola rosea extract, 15-20 parts of astragalus concentrate, 10-15 parts of lyophilized ophiopogon powder, 10-15 parts of tangerine peel superfine powder and 3-7 parts of trehalose.

2. A beauty and skin care enzyme according to claim 1, characterized in that: The invention comprises 45 parts of sea buckthorn pulp, 18 parts of rhodiola rosea extract, 18 parts of astragalus concentrate, 13 parts of lyophilized ophiopogon powder, 13 parts of tangerine peel superfine powder and 5 parts of trehalose.

3. The method for preparing a beauty and skin care enzyme according to claim 1, characterized in that: The steps are as follows: Step 1: fresh seabuckthorn fruits are selected, seeds are removed, and pulped, and pectinase is inactivated by pasteurization to obtain seabuckthorn pulp; Step 2: Selecting Rhodiola rosea and Astragalus membranaceus, respectively, extracting and concentrating them using a water extraction process to obtain a Rhodiola rosea extract and an Astragalus membranaceus concentrate with a soluble solid content of not less than 20%; Step 3, selecting Radix Ophiopogonis and Pericarpium Citri Reticulatae, freeze-drying them and performing ultrafine grinding, and sieving them with a sieve to obtain freeze-dried Radix Ophiopogonis powder and ultrafine Pericarpium Citri Reticulatae powder; Step 4: selecting sea buckthorn fruit, rhodiola rosea extract, astragalus concentrate, lily dextrose freeze-dried powder, and tangerine peel ultrafine powder according to weight ratio, mixing them evenly, and then adding 3% inoculum of plant lactobacillus, 1% suspension of Aspergillus niger spores, and 0.5% inoculum of saccharomyces cerevisiae to obtain a pre-fermentation mixture; Step 5, the pre-fermentation mixture is fermented in stages, the aerobic stage is controlled at 25°C, the rotation speed is 150 rpm, and the fermentation time is 22-26 hours; the anaerobic stage is controlled at 20°C for static fermentation, and the fermentation time is 70-75 hours. When the amylase activity is not less than 250 U / g and the total phenol content is not less than 8 mg / g, the fermentation is terminated to obtain a fermentation liquid; Step 6: The fermentation broth was centrifuged at 8000 rpm for 15 minutes, the supernatant was sterilized by microfiltration using a 0.22 μm filter membrane, and then concentrated by ultrafiltration to obtain a filtrate; Step 7: Add trehalose to the filtrate in proportion, mix well, and then perform vacuum freeze-drying to obtain enzyme powder.

4. The method for preparing a beauty and skin care enzyme according to claim 3, characterized in that: The pasteurization temperature in step 1 is 60° C. and the time is 20 minutes.

5. The method for preparing a beauty and skin care enzyme according to claim 3, characterized in that: The water extraction process of Rhodiola rosea described in step 2 is to add Rhodiola rosea to 60°C pure water according to the ratio of 1KG Rhodiola to 5L pure water, extract for 90 minutes, and concentrate at low temperature under the conditions of vacuum degree of -0.08Mpa and temperature not exceeding 60°C; the water extraction process of Astragalus membranaceus is to add Astragalus membranaceus to 60°C pure water according to the ratio of 1KG Astragalus membranaceus to 8L pure water, extract for 120 minutes, and concentrate under reduced pressure in a 60°C water bath under the condition of vacuum degree of -0.06Mpa.

6. The method for preparing a beauty and skin care enzyme according to claim 3, characterized in that: The sieve used in step 3 is 300 mesh.

7. The method for preparing a beauty and skin care enzyme according to claim 3, characterized in that: The ultrafiltration concentration in step six uses a 10 kDa membrane.

8. The method for preparing a beauty and skin care enzyme according to claim 3, characterized in that: The temperature of the vacuum freeze-drying in step 7 is -50°C.

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