A kind of hard capsule of anti-aging of five grain worm peptide and traditional Chinese medicine compound and its preparation method

Abstract

This invention relates to the field of health food technology, and more particularly to an anti-aging hard capsule of *Polygonum multiflorum* peptide and traditional Chinese medicine compound, and its preparation method. By mass percentage, it comprises the following components: 40%–60% *Polygonum multiflorum* small molecule protein peptide freeze-dried powder, 10%–20% Astragalus polysaccharide extract, 5%–15% Polygonatum polysaccharide extract, 2%–8% Lycium barbarum polysaccharide extract, 1%–5% Panax notoginseng saponin extract, and 3%–10% auxiliary traditional Chinese medicine components and pharmaceutically acceptable excipients. The auxiliary traditional Chinese medicine components are Rhodiola rosea glycoside extract and Echinacea polyphenol extract or licorice powder. This invention's compound follows the principles of traditional Chinese medicine formulation, and the product is rich in small molecule peptides and various active ingredients. It can promote collagen synthesis, clear senescent cells, enhance immunity, resist oxidation, regulate intestinal flora, and improve microcirculation, exhibiting significant anti-aging and anti-fatigue effects. The preparation process is scientifically controllable and has wide applicability.

Description

Technical Field

[0001] This invention relates to the field of health food technology, and in particular to an anti-aging hard capsule made of five grains insect peptide and traditional Chinese medicine compound, and its preparation method. Background Technology

[0002] Aging is a complex physiological process caused by the accumulation of cell damage, decline in immune function, decrease in metabolic function, and imbalance of oxidative stress. Current anti-aging products generally suffer from several technological shortcomings. Most products rely on a single ingredient, resulting in limited efficacy coverage, achieving only single effects such as antioxidation or immune regulation. Small molecule peptides are easily inactivated during high-temperature extraction processes, making product stability difficult to guarantee. The bioavailability of tonic herbs such as astragalus and goji berries is low, and some components may even cause gastrointestinal discomfort. While the raw material of *Polygonum multiflorum* (a type of insect) is rich in protein, its large protein molecules cannot be directly and efficiently absorbed and utilized by the human body.

[0003] Five-grain worms are the larvae of the big-headed fly (Golden-headed Fly) of the Blisteridae family. After being specially bred using fermented feed made from fish, pig lungs, and bullfrogs, their freeze-dried powder contains small-molecule protein peptides with a molecular weight not exceeding 1000 Da, antimicrobial peptides such as cephalosporin, and active amino acids such as arginine and glutamine. These can directly participate in cell repair and the removal of senescent cells. Combining this raw material with tonifying medicinal herbs such as astragalus and wolfberry can further enhance the product's immune-regulating, antioxidant, and metabolic-regulating effects. Current technologies have not yet solved the core problems of insufficient stability and low absorption efficiency of active ingredients. Therefore, developing an anti-aging hard capsule with small-molecule protein peptides as the core, combined with the synergistic effects of various tonifying medicinal herbs and possessing high bioavailability has significant research and application value. Summary of the Invention

[0004] This invention aims to address the problems of existing anti-aging products, such as single ingredients, limited efficacy, easy inactivation of active ingredients, low bioavailability of tonic herbs, and gastrointestinal discomfort. It overcomes the shortcomings of the large molecular protein of the five grain insect, which is difficult to absorb efficiently. By combining small molecular protein peptides with tonic herbs, the stability of the ingredients and absorption efficiency are improved, providing an anti-aging hard capsule with high bioavailability and synergistic effects of immune regulation, anti-oxidation and metabolic regulation.

[0005] The purpose of this invention is to overcome the above-mentioned problems and provide an anti-aging hard capsule of five-grain insect peptide and traditional Chinese medicine compound, and its preparation method. To achieve the above objective, this invention adopts the following technical solution: An anti-aging hard capsule containing a combination of five-grain insect peptides and traditional Chinese medicine, comprising, by weight percentage: 40%–60% freeze-dried five-grain insect small molecule protein peptide powder, 10%–20% Astragalus polysaccharide extract, 5%–15% Polygonatum polysaccharide extract, 2%–8% Lycium barbarum polysaccharide extract, 1%–5% Panax notoginseng saponin extract, 3%–10% auxiliary traditional Chinese medicine components and pharmaceutically acceptable excipients, wherein the auxiliary traditional Chinese medicine components are Rhodiola rosea glycoside extract and Echinacea polyphenol extract or licorice powder.

[0006] Furthermore, when the auxiliary Chinese medicine components are rhodioloside extract and echinacea polyphenol extract, the mass percentage of rhodioloside extract is 3% to 10%, and the mass percentage of echinacea polyphenol extract is 2% to 8%.

[0007] Furthermore, in the freeze-dried powder of small molecule protein peptides from the five grain insects, the proportion of components with a molecular weight of less than 3000 Da is not less than 60%.

[0008] Furthermore, the mass percentage of Astragalus polysaccharide in Astragalus polysaccharide extract is not less than 80%, and the mass percentage of Polygonatum polysaccharide in Polygonatum polysaccharide extract is not less than 75%.

[0009] Furthermore, when the auxiliary Chinese medicine component is licorice powder, the proportion of components with a molecular weight of no more than 1000 Da in the freeze-dried powder of small molecule protein peptides of *Eriocaulon buergerianum* is no less than 60%.

[0010] Furthermore, a method for preparing a hard capsule containing five-grain insect peptides and traditional Chinese medicine compound for anti-aging includes the following steps: (1) When the auxiliary Chinese medicine components are Rhodiola rosea extract and Echinacea polyphenol extract, the grubs are defatted and then added to a compound protease. The mixture is enzymatically hydrolyzed for 2 to 4 hours at a temperature of 40℃~55℃ and a pH of 6.5~8.0. The enzyme is then inactivated, the supernatant is collected by centrifugation, and the mixture is then freeze-dried under vacuum to obtain grubs small molecule protein peptide freeze-dried powder. When the auxiliary Chinese medicine components are licorice powder, the grubs are raised using fermented feed made from fish, pig lungs, and bullfrogs. The raised grubs are then freeze-dried under vacuum at -40℃ and then pulverized at ultra-low temperature to 80~100 mesh to obtain grubs small molecule protein peptide freeze-dried powder. (2) When the auxiliary Chinese medicine components are rhodioloside extract and echinacea polyphenol extract, the corresponding Chinese medicinal materials are subjected to low-temperature ultrafine grinding at a temperature of -10℃ to 5℃ to the particle size of the material. No more than 30 , the water extraction and alcohol precipitation technology or membrane separation technology is adopted to extract and prepare the traditional Chinese medicine extract powder; when the auxiliary traditional Chinese medicine component is licorice powder, the polygonatum sibiricum is ultrafinely pulverized to 100 meshes, and the licorice is ground into powder at low temperature; the astragalus membranaceus and wolfberry are extracted by the water extraction process with a material-liquid ratio of 1:10 and at 80°C, and after extraction twice, the filtrates are combined and concentrated to 5:1 to obtain the astragalus membranaceus extract and wolfberry extract; the notoginseng is extracted by refluxing with 60% ethanol, and the extract yield is not less than 15% to obtain the notoginseng extract. (3) The freeze-dried powder of the small molecule protein peptide of the sarcophaga peregrina, the traditional Chinese medicine extract powder, the auxiliary traditional Chinese medicine component and the pharmaceutically acceptable excipients are mixed evenly, granulated by the wet granulation method, dried and sized to obtain dry granules. (4) The dry granules are filled into hard capsule shells to prepare the anti-aging hard capsules.

[0011] The compound components of the present invention follow the compatibility principle of monarch, minister, assistant and guide, and the raw material ratios and core functions are as follows: The monarch drug is the freeze-dried powder of the sarcophaga peregrina, with a ratio of 50%, which can provide small molecule protein peptides with a molecular weight ≤ 1000 Da, promote the synthesis of collagen and elastin, and the antibacterial peptides contained can remove SA-β-gal positive senescent cells; the minister drugs are the astragalus membranaceus extract (5:1) and wolfberry powder, with ratios of 20% and 15% respectively. The astragalus polysaccharide in the astragalus membranaceus extract can activate the activities of macrophages and T cells, and astragaloside can improve mitochondrial energy metabolism and counteract cell energy exhaustion. The wolfberry polysaccharide in the wolfberry powder can scavenge DPPH and superoxide anion free radicals, and zeaxanthin can protect the retina and resist skin photoaging; the assistant drugs are the polygonatum sibiricum powder and notoginseng powder (200 meshes), with ratios of 10% and 4% respectively. The polygonatum polysaccharide in the polygonatum sibiricum powder can synergistically enhance immunity, and polygonatum saponin can regulate the intestinal flora and improve the metabolic senescence of the intestine-skin axis. The total saponins of notoginseng in the notoginseng powder can improve microcirculation, promote the delivery of protein peptides, and at the same time inhibit platelet aggregation and reduce the risk of thrombosis; the guiding drug is the licorice powder, with a ratio of 1%, and glycyrrhizin can relieve the warm and dry nature of astragalus membranaceus and polygonatum sibiricum, and glycyrrhetinic acid can inhibit the inflammatory factors of IL-6 and TNF-α.

[0012] The advantages of the present invention are as follows: 1. This invention achieves multi-dimensional anti-aging by combining small molecule protein peptides from *Eriocaulon buergerianum*, antimicrobial peptides, astragalus polysaccharides, wolfberry polysaccharides, zeaxanthin, polygonatum saponins, and notoginseng saponins. It can promote the synthesis of skin collagen by 25%, reduce SA-β-gal positive senescent cells by at least 30%, activate the proliferation capacity of spleen lymphocytes by 40%, increase the phagocytic rate of macrophages by 35%, make the DPPH free radical inhibition rate of wolfberry polysaccharides by at least 70%, protect the skin and retina, increase the abundance of intestinal bifidobacteria by 2 times, reduce fasting blood glucose by 10%-15% to improve insulin resistance, and at the same time increase the amount of collagen synthesis in diabetic ulcers by 50% and shorten the wound healing time by 30%.

[0013] 2. This invention uses ultra-low temperature pulverization and freeze-drying processes and is formulated with licorice to retain the activity of small molecule peptides with a molecular weight of no more than 1000 Da and antimicrobial peptides, while reducing the gastrointestinal irritation of astragalus and polygonatum. The product has a wide range of applications and can be used for sub-healthy people over 40 years old, middle-aged and elderly people with chronic inflammation, people recovering from surgery, and women's anti-aging maintenance. Attached Figure Description

[0014] The accompanying drawings, which form part of this application, are used to provide a further understanding of the application and to make other features, objects, and advantages of the application more apparent. The illustrative embodiments and descriptions of this application are used to explain the application and do not constitute an undue limitation of the application.

[0015] In the attached diagram: Figure 1 This is a flowchart of the preparation method of a hard capsule for anti-aging with a traditional Chinese medicine compound containing five grain insect peptides in Example 1. Detailed Implementation

[0016] The present invention will now be described in detail and specifically through specific embodiments to enable a better understanding of the invention. However, the following embodiments do not limit the scope of protection of the present invention.

[0017] Example 1 A hard capsule containing a compound of five grain insect peptides and traditional Chinese medicine for anti-aging, by weight percentage, is composed of the following components: 50% freeze-dried five grain insect powder, 20% Astragalus extract (5:1), 15% wolfberry powder, 10% Polygonatum powder, 4% Panax notoginseng powder (200 mesh), 1% licorice powder, and 5% starch paste, wherein the proportion of components with a molecular weight of not more than 1000 Da in the freeze-dried five grain insect powder is not less than 60%.

[0018] like Figure 1 As shown, the specific steps of the preparation method of the above-mentioned anti-aging hard capsules are as follows: Five-grain insects were raised using fermented feed made from fish, pig lungs, and bullfrogs. After being raised, the five-grain insects were vacuum freeze-dried at -40℃ and then pulverized at ultra-low temperature to 80-100 mesh to obtain freeze-dried five-grain insect powder.

[0019] Polygonatum was ultra-finely pulverized to 100 mesh, and licorice was ground into powder at low temperature. Astragalus and wolfberry were extracted using a water extraction process with a material-to-liquid ratio of 1:10 and a temperature of 80℃. After two extractions, the filtrates were combined and concentrated to a ratio of 5:1 to obtain Astragalus extract and wolfberry extract. Panax notoginseng was extracted by reflux with 60% ethanol, and the extract yield was not less than 15% to obtain Panax notoginseng extract.

[0020] The freeze-dried powder of five grains insects was mixed evenly with the extracts of various Chinese herbal medicines and licorice powder according to the formula. 5% starch slurry was added as a binder and wet granulation was carried out. The mixture was dried at 50°C until the moisture content was ≤5%. Then, the mixture was granulated to obtain dry granules.

[0021] The obtained dry granules are filled into 0# or 1# hard capsule shells, and the amount of each capsule is controlled to be 0.5g, so as to obtain anti-aging hard capsules.

[0022] The prepared anti-aging hard capsules were used for experimental verification. After continuous gavage administration to aging model mice for 30 days, telomere length increased by 18% and the inflammatory factor IL-6 decreased by 35%. A human trial was conducted on 60 subjects. After continuous use for 4 weeks, the subjects' fatigue scores decreased by 40% and skin elasticity increased by 20%.

[0023] The specific embodiments of the present invention have been described in detail above, but they are merely examples, and the present invention is not equivalent to the specific embodiments described above. For those skilled in the art, any equivalent modifications and substitutions to the present invention are also within the scope of the present invention. Therefore, all equivalent transformations and modifications made without departing from the spirit and scope of the present invention should be covered within the scope of the present invention.

Claims

1. A kind of hard capsule of anti-aging of five grain worm peptide and traditional Chinese medicine compound, it is characterized by, By mass percentage, it consists of the following components: 40%–60% freeze-dried small molecule protein peptide powder of *Polygonatum sibiricum*, 10%–20% Astragalus polysaccharide extract, 5%–15% Polygonatum polysaccharide extract, 2%–8% Lycium barbarum polysaccharide extract, 1%–5% Panax notoginseng saponin extract, 3%–10% auxiliary traditional Chinese medicine components and pharmaceutically acceptable excipients, wherein the auxiliary traditional Chinese medicine components are Rhodiola rosea glycoside extract and Echinacea polyphenol extract or licorice powder.

2. The anti-aging hard capsule of five-grain insect peptide and traditional Chinese medicine compound according to claim 1, characterized in that, When the auxiliary Chinese medicine components are rhodioloside extract and echinacea polyphenol extract, the mass percentage of the rhodioloside extract is 3% to 10%, and the mass percentage of the echinacea polyphenol extract is 2% to 8%.

3. The anti-aging hard capsule of five-grain insect peptide and traditional Chinese medicine compound according to claim 2, characterized in that, In the freeze-dried powder of small molecule protein peptides from the five grain insects, the proportion of components with a molecular weight of less than 3000 Da is not less than 60%.

4. The anti-aging hard capsule of five-grain insect peptide and traditional Chinese medicine compound according to claim 2, characterized in that, The Astragalus polysaccharide extract contains at least 80% Astragalus polysaccharide by mass, and the Polygonatum polysaccharide extract contains at least 75% Polygonatum polysaccharide by mass.

5. The anti-aging hard capsule of five-grain insect peptide and traditional Chinese medicine compound according to claim 1, characterized in that, When the auxiliary Chinese medicine component is licorice powder, the proportion of components with a molecular weight of not more than 1000 Da in the freeze-dried powder of small molecule protein peptides of the five grain insects is not less than 60%.

6. A method for preparing a hard capsule containing five-grain insect peptide and traditional Chinese medicine compound for anti-aging according to any one of claims 1-5, characterized in that, Includes the following steps: (1) When the auxiliary Chinese medicine components are Rhodiola rosea extract and Echinacea polyphenol extract, the grubs are defatted and then added to a compound protease. The mixture is enzymatically hydrolyzed for 2 to 4 hours at a temperature of 40℃~55℃ and a pH of 6.5~8.

0. The enzyme is then inactivated, the supernatant is collected by centrifugation, and the mixture is then freeze-dried under vacuum to obtain grubs small molecule protein peptide freeze-dried powder. When the auxiliary Chinese medicine components are licorice powder, the grubs are raised using fermented feed made from fish, pig lungs, and bullfrogs. The raised grubs are then freeze-dried under vacuum at -40℃ and then pulverized at ultra-low temperature to 80~100 mesh to obtain grubs small molecule protein peptide freeze-dried powder. (2) When the auxiliary Chinese medicine components are rhodioloside extract and echinacea polyphenol extract, the corresponding Chinese medicinal materials are subjected to low-temperature ultrafine grinding at a temperature of -10℃ to 5℃ to the particle size of the material. No more than 30 The traditional Chinese medicine extract powder was prepared by water extraction and alcohol precipitation or membrane separation technology. When the auxiliary traditional Chinese medicine component was licorice powder, Polygonatum was ultra-finely pulverized to 100 mesh, and licorice was ground into powder at low temperature. Astragalus and Lycium were extracted by water extraction at 80℃ with a material-to-liquid ratio of 1:

10. After two extractions, the filtrates were combined and concentrated to 5:1 to obtain Astragalus extract and Lycium extract. Panax notoginseng was extracted by reflux with 60% ethanol, and the extract yield was not less than 15% to obtain Panax notoginseng extract. (3) Mix the freeze-dried powder of small molecule protein peptides of the five grain insects, the powder of traditional Chinese medicine extract, the auxiliary traditional Chinese medicine components and pharmaceutically acceptable excipients evenly, and then granulate by wet granulation, drying and granulation to obtain dry granules; (4) The dry granules are filled into a hard capsule shell to obtain the anti-aging hard capsule.

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