Endothelium culture system

A culture system and endothelial culture medium technology, applied in the field of endothelial culture system, can solve the problems of being easily contaminated by heterocysts, insufficient growth activity of endothelial cells of large blood vessels, etc., and achieve the effects of promoting growth activity and reducing pollution.

Inactive Publication Date: 2016-06-08
ICELL SHANGHAI BIOSCI CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

[0002] Endothelial cells or vascular endothelium is a thin layer of specialized epithelial cells, composed of a layer of flat cells, polygonal, the edges of the cells are jagged, and fit together, it forms the inner wall of blood vessels, is the blood and other The interface of the blood vessel wall (single-layer squamous epithelium), endothelial cells are along the entire circulatory system, from the heart to the smallest capillaries; one of the most important functions of vascular endothelial cells is to participate in the formation of blood vessels, and in many cardiovascular diseases, di

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Embodiment Construction

[0010] The endothelial culture system of the present invention comprises an endothelial medium and an endothelial additive; the composition and concentration of the endothelial medium are: sodium chloride 6800mg / L, potassium chloride 400mg / L, calcium chloride 200mg / L, magnesium sulfate 200mg / L, sodium dihydrogen phosphate 140mg / L, L-arginine 70mg / L, L-histidine 20mg / L, L-lysine 70mg / L, DL-tryptophan 20mg / L, DL-benzene Alanine 50mg / L, DL-methionine 30mg / L, DL-serine 50mg / L, DL-threonine 60mg / L, DL-leucine 120mg / L, DL-isoleucine 40mg / L, DL -Valine 50mg / L, DL-Glutamic Acid 150mg / L, DL-Aspartic Acid 60mg / L, DL-α-Alanine 50mg / L, L-Proline 40mg / L, L-Hydroxy Proline 10mg / L, Glycine 50mg / L, Sodium Acetate 83mg / L, Phenol Red 15mg / L, L-Tyrosine 40mg / L, L-Cystine 20mg / L, Niacin (Vitamin B3) 0.025mg / L, Nicotinamide 0.025mg / L, Pyridoxine Hydrochloride 0.025mg / L, Pyridoxal Hydrochloride 0.025mg / L, Vitamin B10.01mg / L, Riboflavin (Vitamin B2) 0.01mg / L, Calcium Pantothenate 0.01 mg / L, meso...

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Abstract

The invention discloses an endothelium culture system. The endothelium culture system comprises an endothelium culture medium and an endothelium additive, wherein the endothelium additive comprises components by concentration as follows: 5 ng/ml of a recombinant human epidermal growth factor, 10 ng/ml of hydrocortisone, 10 ng/ml of a recombinant human vascular endothelial growth factor, 5 ng/ml of a recombinant human basic fibroblast growth factor, 5 ng/ml of an R3 insulin-like growth factor, 50 mu g/ml of vitamin C, 50 mu g/ml of heparin, 10 mu g/ml of recombinant human insulin and 5 mu g/ml of human total iron transferrin. The endothelium culture system can promote growth activity of great vascular endothelial cells in a 5% serous environment, partially inhibit activity of fibroblasts and smooth muscle cells and reduce parenchyma cell pollution.

Description

technical field [0001] The invention particularly relates to an endothelial culture system, which belongs to the technical field of large blood vessel endothelial cell culture. Background technique [0002] Endothelial cells or vascular endothelium is a thin layer of specialized epithelial cells, composed of a layer of flat cells, polygonal, the edges of the cells are jagged, and fit together, it forms the inner wall of blood vessels, is the blood and other The interface of the blood vessel wall (single-layer squamous epithelium), endothelial cells are along the entire circulatory system, from the heart to the smallest microvessels; one of the most important functions of vascular endothelial cells is to participate in the formation of blood vessels, and in many cardiovascular diseases, diabetes In many cases, vascular organic diseases caused by vascular endothelial cells are caused by apoptosis, necrosis or non-proliferation of vascular endothelial cells. Therefore, vascular...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/069C12N2500/12C12N2500/14C12N2500/16C12N2500/25C12N2500/30C12N2500/32C12N2500/34C12N2500/35C12N2500/36C12N2500/38C12N2500/40C12N2500/60C12N2501/105C12N2501/11C12N2501/115C12N2501/165C12N2501/91C12N2501/999
Inventor 王悦
Owner ICELL SHANGHAI BIOSCI CO LTD
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