Endothelium culture system

Abstract

The invention discloses an endothelium culture system. The endothelium culture system comprises an endothelium culture medium and an endothelium additive, wherein the endothelium additive comprises components by concentration as follows: 5 ng / ml of a recombinant human epidermal growth factor, 10 ng / ml of hydrocortisone, 10 ng / ml of a recombinant human vascular endothelial growth factor, 5 ng / ml of a recombinant human basic fibroblast growth factor, 5 ng / ml of an R3 insulin-like growth factor, 50 mu g / ml of vitamin C, 50 mu g / ml of heparin, 10 mu g / ml of recombinant human insulin and 5 mu g / ml of human total iron transferrin. The endothelium culture system can promote growth activity of great vascular endothelial cells in a 5% serous environment, partially inhibit activity of fibroblasts and smooth muscle cells and reduce parenchyma cell pollution.

Description

technical field [0001] The invention particularly relates to an endothelial culture system, which belongs to the technical field of large blood vessel endothelial cell culture. Background technique [0002] Endothelial cells or vascular endothelium is a thin layer of specialized epithelial cells, composed of a layer of flat cells, polygonal, the edges of the cells are jagged, and fit together, it forms the inner wall of blood vessels, is the blood and other The interface of the blood vessel wall (single-layer squamous epithelium), endothelial cells are along the entire circulatory system, from the heart to the smallest microvessels; one of the most important functions of vascular endothelial cells is to participate in the formation of blood vessels, and in many cardiovascular diseases, diabetes In many cases, vascular organic diseases caused by vascular endothelial cells are caused by apoptosis, necrosis or non-proliferation of vascular endothelial cells. Therefore, vascular...

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Problems solved by technology

[0002] Endothelial cells or vascular endothelium is a thin layer of specialized epithelial cells, composed of a layer of flat cells, polygonal, the edges of the cells are jagged, and fit together, it forms the inner wall of blood vessels, is the blood and other The interface of the blood vessel wall (single-layer squamous epithelium), endothelial cells are along the entire circulatory system, from the heart to the smallest capillaries; one of the most important functions of vascular endothelial cells is to participate in the formation of blood vessels, and in many cardiovascular diseases, diabetes In many cases, vascular organic diseases caused by vascular endothelial cells are caused by apoptosis, necrosis or non-proliferation of vascular endothelial cells. Therefore, vascular endothelial cells have become the focus of many researches. These studies and experiments It is often necessary to use a large number of vascular endothelial cells; in the prior art, using M199 basal medium and 10% fetal bovine serum as the medium to cultivate endothelial cells, the growth activity of the large vascular endothelial cells is not enough, and it is easy to be polluted by miscellaneous cells