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Novel cell recovery agent

A technology of cells and reagents, applied in the direction of cell culture active agents, animal cells, microorganisms, etc., can solve the problems of recovery survival rate (low recovery rate, poor versatility, poor activity, etc.), to achieve easy quality control, safe and reliable use, good The effect of recovery rate

Active Publication Date: 2017-05-10
焕生汇生物基因技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 1. Poor versatility, a resuscitation reagent is only suitable for one type of cell, or only for a few similar cells;
[0009] 2. After the cells are recovered, the recovery survival rate (recovery rate) is low, and the activity is poor

Method used

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Embodiment Construction

[0020] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0021] 1. Preparation of relevant reagents

[0022] The composition and dosage of each group in table 1

[0023]

[0024] In the resuscitation reagent of the present invention, we choose recombinant human serum albumin, and mix recombinant human serum albumin and RPMI-1640 medium according to the volume ratio of 2:8, which does not contain animal components such as serum, and is safe and reliable to use.

[0025] In addition, in order to better maintain the activity of the resuscitated cells, we have repeatedly verified, and finally selected recombinant human epidermal growth factor, recombinant human basic fibroblast growth factor and vitamin D as supplementary ingredients, in order to verify the most appropriate factor concentration , we carried out different combinations of the above components in order to verify the specific recovery effect.

[0026] 2. Prepa...

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PUM

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Abstract

The invention discloses a novel cell recovery agent. The novel cell recovery agent comprises the following ingredients: 100 ng of a recombinant human epidermal growth factor, 200 ng of a recombinant human basic fibroblast growth factor, 3000 ng of vitamin D, 2 ml of a recombinant human serum albumin, and 8 ml of an RPMI-1640 culture medium. The novel cell recovery agent has the beneficial effects that (1) the novel cell recovery agent does not contain animal ingredients and is safe and reliable to use; (2) the ingredients are completely clear and definite, and the quality is convenient to control; (3) the novel cell recovery agent is a ready-to-use type, and the operation is more convenient; (4) the applicable cell type is not limited; and (5) better cell viability and thawing rate are achieved when the cell is recovered.

Description

technical field [0001] The invention relates to a cell resuscitation reagent, in particular to a novel cell resuscitation reagent, which belongs to the field of biotechnology. Background technique [0002] There are two types of cryopreservation reagents used for cell cryopreservation: serum-containing and serum-free. Serum has the disadvantages of complex components, unstable quality, and high price, so serum-free cryopreservation and resuscitation technology has become a development trend. The present invention is mainly aimed at the research of serum-free cryopreservation and resuscitation technology. [0003] When the cells are cryopreserved, the cells are generally suspended in the cryopreservation reagent, and then the temperature is lowered by a gradient cooling method, and the cooling rate is about 1°C / min. [0004] When resuscitating cryopreserved cells, generally place the cryopreservation tube directly at 37°C to thaw quickly, then add complete growth medium to r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/07
CPCC12N5/06C12N2500/38C12N2501/11C12N2501/115C12N2501/998
Inventor 姚玲玲杨敏孟得龙杨苗
Owner 焕生汇生物基因技术(北京)有限公司
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