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Novel cell cryopreservation reagent

A cell and cryopreservation technology, applied in the field of cell cryopreservation reagents, can solve the problems of unsafe use, inconvenient operation, easy pollution, etc., and achieve the effects of safe and reliable use, convenient operation, and completely clear components.

Inactive Publication Date: 2017-02-22
北京焕生汇生物技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1. It contains animal ingredients and is unsafe to use;
[0005] 2. When using, it needs to be used and prepared immediately, which is inconvenient to operate and easy to pollute

Method used

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  • Novel cell cryopreservation reagent
  • Novel cell cryopreservation reagent
  • Novel cell cryopreservation reagent

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Experimental program
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Embodiment Construction

[0017] The present invention will be specifically introduced below in conjunction with specific embodiments.

[0018] 1. Preparation of relevant reagents

[0019] The composition and dosage of each group in table 1

[0020]

[0021] The existing cell cryopreservation reagents generally have a formula of fetal bovine serum combined with DMEM medium, and a certain amount of DMSO is added at the same time. A lot of questions.

[0022] In the present invention, we choose recombinant human serum albumin to replace fetal bovine serum, and mix recombinant human serum albumin and DMEM medium according to the volume ratio of 2:8. In addition, in order to better maintain the cryopreserved cells Activity, after repeated verification, we finally selected recombinant human epidermal growth factor, recombinant human basic fibroblast growth factor and human hepatocyte growth factor as supplementary components. In order to verify the most appropriate factor concentration, we carried out ...

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Abstract

The invention discloses a novel cell cryopreservation reagent which comprises, 100ng of recombinant human epidermal growth factors, 500ng of recombinant human basic fibroblast growth factors, 300ng of recombinant human hepatocyte growth factors, 0.5ml of DMSO (dimethyl sulfoxide), 2ml of recombinant human serum albumin and 8ml of DMEM (Dulbecco's modified eagle medium). The novel cell cryopreservation reagent has the advantages that the novel cell cryopreservation reagent is free from animal components and safe and reliable to use, components are completely definite, quality control is facilitated, the reagent is ready to use and can be directly used for cell resuspension after cell counting, the reagent is more convenient to operate without additional preparation, applicable cell types are unlimited, and cells can be maintained in long-term safe storage in a -196 DEG C liquid nitrogen environment.

Description

technical field [0001] The invention relates to a cell cryopreservation reagent, in particular to a novel cell cryopreservation reagent, belonging to the field of biotechnology. Background technique [0002] There are two types of cryopreservation reagents used for cell cryopreservation: serum-containing and serum-free. Serum has the disadvantages of complex components, unstable quality, and high price, so serum-free cryopreservation technology has become a development trend. The present invention is mainly aimed at the research of the serum-free cryopreservation technology. [0003] Existing cell cryopreservation reagents mainly have the following two problems: [0004] 1. It contains animal ingredients and is unsafe to use; [0005] 2. When using, it needs to be used and prepared immediately, which is inconvenient to operate and easy to pollute. Contents of the invention [0006] In order to solve the deficiencies of the prior art, the purpose of the present invention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 姚玲玲杨敏孟得龙杨苗
Owner 北京焕生汇生物技术研究院有限公司
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