Serum-free culture medium for mesenchymal stem cells

Abstract

The invention provides a serum-free culture medium for mesenchymal stem cells. The serum-free culture medium specifically comprises a lipid mixture, glutamine, hydrocortisone, zinc sulfate, sodium butyrate, ferric citrate, vitamin C, recombinant epidermal growth factors, recombinant alkaline fibroblast growth factors, RGD oligopeptides, palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, lithium chloride, L-glutathione, a soybean pancreatin inhibitor, beta-mercaptoethanol, ethanolamine, sodium selenite, Hepes, sodium bicarbonate and a basic culture medium. The serum-free culture medium is free of human and animal resources, clear in chemical component, rich in nutrition, capable of achieving UC-MSCs rapid proliferation, and capable of solving the problem of cells number insufficiency and maintaining the biological features and immunophenotyping stability of the mesenchymal stem cells.

Description

technical field [0001] The invention relates to the technical field of medium, in particular to a serum-free medium for mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are derived from the mesoderm in the early stage of development and are a type of non-hematopoietic stem cells that are widely distributed in bone marrow, subcutaneous fat, periosteum, muscle, synovium, synovial fluid, liver, peripheral tissue, umbilical cord, cord blood and placenta. MSCs have high self-renewal ability and multi-directional differentiation potential, and can be cultured and expanded in vitro. They can not only support the growth of hematopoietic stem cells, but also have the function of immune regulation; under different induction conditions, they can differentiate into bone, cartilage, and muscle in vitro. , nerve, myocardium, endothelium and fat, etc., still have multi-directional differentiation potential after continuous subculture and cryopreservation...

AI Technical Summary

Problems solved by technology

The first generation is a serum-free medium in a general sense, using various biological materials that can replace the function of serum, containing a large amount of animal-derived proteins and unknown components, such as animal or human-derived platelet lysates, etc.; its advantage is compared with containing Serum medium has higher experimental accuracy, repeatability, and stability, but because the chemical composition of the added substances is not clear, most of them contain a large amount of animal-derived protein, which is not conducive to the separation and purification of the target protein, and the cost is high

The second generation is a serum-free and animal-derived protein-free medium. Its additives do not use animal-derived protein at all, but replace it with various recombinant proteins or animal and plant protein hydrolyzates; its advantage is that its stability is improved compared with the first generation. The cost is reduced, and the effect is also improved accordingly. However, due to the high cost, it is only suitable for scientific research users with small demand, and it is difficult to be used by large-scale production enterprises.

The third generation is a limited-component medium, also known as double-free medium; the added components are completely free of serum, protein-free or extremely low in protein content, and the protein contained is clearly defined; the third-generation SFM has obvious advantages, cell culture It is easy to be consistent with the production, the separation and purification of the target protein is easier, and the cost is greatly reduced. However, at the current stage, such products have the disadvantage of insufficient cell passage ability. The cells have high specificity, so there are few cell lines suitable for culture, and product development is extremely difficult

The fourth generation is a medium with limited chemical components. The added ingredients do not contain serum or any protein. The compound is mainly used to replace unstable protein substances. It can be sterilized at high temperature and is suitable for the growth of a variety of different cell types. Currently, it is an all-round medium. There is no such product on the market yet, and it is still in the research and development stage

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