Model for simulating dermatophyte infection in vitro and establishment method and application thereof

Abstract

The invention discloses a model for simulating dermatophyte infection in vitro and an establishment method and application thereof, and belongs to the technical field of bioengineering. The model forsimulating dermatophyte infection in vitro comprises matrix glue, an epidermal analogue embedded in the solidified matrix glue and dermatophyte hyphae, the epidermal analogue is of a relatively complete solid sphere structure, and the exterior of the epidermal analogue is a cell layer which is composed of substrate layer cells and substrate upper layer cells and distributed in a surrounding mode,and a keratinization sample non-cell layer is arranged inside; hyphae invade basal layer cells outside the sphere structure and do not invade or only invade a small amount of basal layer cells, and donot invade the keratinization sample non-cell layer inside the sphere structure; the model can effectively simulate the state that dermatophyte infects normal skin tissue, the infection process of the model can be directly observed under a phase contrast microscope, the infection situation can be detected through omics methods such as high-throughput RNA sequencing, and the model can be effectively used for researching the pathogenic mechanism of dermatophyte infection and screening anti-dermatophyte drugs.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to an in vitro model for simulating dermatophyte infection and a method for establishing the same, as well as its application in the research of pathogenic mechanism of dermatophyte infection and in the screening of anti-dermatophyte drugs. Background technique [0002] Dermatophyte infection, commonly known as "beriberi", is the most common type of human fungal infection. The infection is usually limited to the epidermis, and most of it is caused by Trichophyton rubrum, which specifically eats human epidermal tissue. The clinical and pathological features of dermatophyte infection are chronic recurrent infection, thickening and exfoliation of keratinized skin tissue, and mild inflammatory response, but it is difficult for the human body to eradicate itself, and a good adaptability has been formed between the pathogen and the human host. Although not a serious life...

AI Technical Summary

Problems solved by technology

However, in the infection model established by using this structure, after dermatophyte infection, in the early stage of infection, due to the blocking of the outermost stratum corneum, it is difficult for the dermatophyte to directly contact the underlying cells, so it is difficult to trigger obvious cellular responses, which hinders Research on the cellular immune response to infection; and when the infection time is prolonged so that the fungus can penetrate the stratum corneum and directly contact the cells, it will cause a large number of fungus to multiply and adhere tightly to the stratum corneum of the epidermis Substances are not conducive to the use of high-throughput omics methods (such as high-throughput RNA sequencing) for in-depth mining of infection mechanisms and exploration of new pathogenic mechanisms

In addition, the construction of the model produced by the gas-liquid intersecting culture method requires a large number of initial skin cells, and the construction process is long and complicated, so it is difficult to apply it on a large scale in the study of dermatophyte infection; and because of the gas-liquid intersecting The particularity of the culture method (that is, no liquid is added to the top layer of gas-liquid intersecting culture, directly in contact with air, and liquid is added to the lower layer), makes it difficult to observe the progress of infection under a microscope for the model established by this method

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