Pigment-containing artificial skin detection model and preparation method thereof

A technology of artificial skin and detection model, which is applied in the field of tissue engineering in biomedical engineering, can solve the problem of artificial skin detection model without pigmentation and its application reports, can not be given to keratinocytes and melanocytes, and the production cost increases, etc. problem, to achieve the effect of small shrinkage, low cost and simple production

Active Publication Date: 2010-08-04
GUANGDONG BOXI BIO TECH CO LTD
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

The Chinese patent "Tissue-engineered skin with adjustable pigment secretion and its construction method" (application number: 03134539.5) prepares a tissue-engineered skin, which is mainly used for clinical treatment, rather than a standardized cosmetic skin test model, which contains dermal components Fibroblasts increase the production cost, and the biological support material is collagen gel, which cannot provide sufficient nutrition for keratinocytes and melanocytes to form the epidermal structure required for the detection model, and the mechanical strength is poor
[0006] After searching, there is no report on the artificial skin detection model containing pigment and its application

Method used

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Experimental program
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Effect test

Embodiment 1

[0026] Step 1. Prepare culture medium. Mix the same volume of MCDB153 commercial culture medium and K-SFM commercial culture medium as the base solution; then add 250 ng of basic fibroblast growth factor, 10 ng of insulin, 5 μg of epidermal growth factor, and hydrocortisone according to the standard of 500 ml of base solution 250μg, vascular endothelin 2.5μg, adenine 15mg, transferrin 5mg, vitamin C 10mg, make the basal medium; then use the basal medium to prepare the following medium:

[0027] Medium a: the basal medium contains bovine pituitary extract at a concentration of 25 μg / ml;

[0028] Medium b: the basal medium contains calcium chloride at a concentration of 0.5mM;

[0029] Medium c: the basal medium contains calcium chloride at a concentration of 1.5mM;

[0030] Medium d: the basal medium contains calcium chloride at a concentration of 2.5mM;

[0031] Step 2. Preparation of biodegradable material support membrane (select biorecombination material): at 4°C, compou...

Embodiment 2

[0036] Step 1. Preparation of medium: Mix the same volume of MCDB153 commercial culture medium and K-SFM commercial culture medium as the base solution; then add 500ng of basic fibroblast growth factor and 40ng of insulin according to the standard of 500ml of base solution, and the epidermal cells will grow Factor 10μg, hydrocortisone 400μg, vascular endothelin 3μg, adenine 20mg, transferrin 10mg, vitamin C 30mg, make basal medium; then use the basal medium to prepare the following medium:

[0037] Medium a: the basal medium contains bovine pituitary extract at a concentration of 50 μg / ml;

[0038] Medium b: the basal medium contains calcium chloride at a concentration of 0.5mM;

[0039] Medium c: the basal medium contains calcium chloride at a concentration of 1 mM;

[0040] Medium d: the basal medium contains calcium chloride at a concentration of 2 mM;

[0041] Step 2. Preparation of biodegradable material support membrane (selection of biologically derived materials): ta...

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Abstract

The invention relates to a pigment-containing artificial skin detection model and a preparation method thereof. The artificial skin detection model is an epidermal layer structure with activity, which is constructed by compositely inoculating cutin formative cells and melanophores to the surface of a support membrane made from a biodegradable material through culture and comprises a stratum basale, a stratum spinosum, a stratum granulosum and a stratum corneum, and a basilar membrane structure is formed between the epidermal layer and the biodegradable material positioned below the epidermal layer. Compared with the conventional product, the invention can better simulate the pigment components of natural skin and more accurately reflect the whitening and sunscreen effect of cosmetics because of containing the melanophores, nourish the cutin formative cells by using the support membrane made from the biodegradable material as an enderonic layer, control the contraction of the epidermal layer with the strength and enhance the stability of the detection model and has low raw material cost, short manufacturing period and mass production; and in addition, the prepared artificial skin detection model can be directly used for the efficacy detection of the cosmetics and the skin irritation detection of textiles, the cosmetics and chemicals.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering in biomedical engineering, and in particular relates to a pigment-containing artificial skin detection model and a preparation method thereof. Background technique [0002] Cosmetics are used more and more in modern life, and cosmetic testing technology is directly related to the skin-beautifying effect and safety of cosmetics, so testing technology is very important in cosmetics development. Previously, most cosmetics were tested on living animals. Due to many problems, according to the EU Cosmetics Directive (76 / 768 / EEC), starting from 2009, the sale of cosmetics developed only through animal experiments is completely prohibited. The global cosmetics industry has begun to adopt a more sensitive and accurate detection method, the artificial skin detection model. [0003] The application of artificial skin detection model in cosmetic safety and functional effect detection has been acce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071G01N33/50A61L27/60
Inventor 杨改叶
Owner GUANGDONG BOXI BIO TECH CO LTD
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