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Facial mask with excellent moisturizing and repairing effects

A facial mask and moisturizing agent technology, which is applied in the direction of medical preparations containing active ingredients, skin care preparations, and cosmetic preparations, etc. It can solve the problem of EGF's difficulty in mass production, difficulty in fully exerting its efficacy, and lack of wide application. problem, to achieve the effect of protecting capillaries and collagen, excellent cell proliferation and differentiation, and promoting cell proliferation and differentiation

Active Publication Date: 2021-03-26
四川省恩乐生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Epidermal Growth Factor (EGF) is an important cell growth factor secreted by the human body and has strong physiological activity. However, EGF has not been widely used in the fields of medicine and cosmetics. The reason is that EGF is difficult to achieve in batches. Chemical production: At present, the extraction methods of EGF are mainly divided into two types, one is from animal sources, such as mouse jaw, bovine brain, etc., and the other is from the culture supernatant of stem cells. The two methods are obtained The content of EGF is very small, and the cost is extremely high
In addition, the molecular weight of the usual EGF is about 6000 Daltons, the molecular weight is relatively large, and the penetrating power to the skin is poor, and its efficacy is difficult to fully exert

Method used

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  • Facial mask with excellent moisturizing and repairing effects
  • Facial mask with excellent moisturizing and repairing effects
  • Facial mask with excellent moisturizing and repairing effects

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Obtaining the target DNA

[0034] (1) The gene sequence of the target gene is:

[0035] Using 5'-GCAAGCGCATTGCACATGTACGGCTATAGCAGATA-3' (SEQ ID NO: 1) to use the messenger RNA transcribed from the target gene as a template, reverse transcribe it into complementary single-stranded DNA, and then synthesize double-stranded DNA under the action of T4 DNA ligase, that is, the target gene Gene.

[0036] (2) PCR amplification

[0037] Denaturation: High temperature dissociates double-stranded DNA into single strands (94°C, 30s);

[0038] Annealing: The primer (pACT2 GAL4 AD pACT2-R) binds to the complementary region of the template DNA at low temperature to form a hybrid molecule (55°C, 30s);

[0039] Extension: moderate temperature extension, in DNA polymerase, dNTP, Mg 2+ In the presence, DNA polymerase catalyzes the 5' to 3' extension of the DNA strand starting from the primer, and synthesizes a DNA sub-strand complementary to the template DNA strand (70-72°C...

Embodiment 2

[0041] Embodiment 2: Electrophoresis detection and recovery purification

[0042] (1) Electrophoresis

[0043] ① Prepare an appropriate amount of buffer for electrophoresis and gel preparation (1X TAE Buffer)

[0044]② Accurately weigh the agarose powder (1g) according to the amount of gel to be prepared and the concentration of the gel, and add it to an appropriate Erlenmeyer flask.

[0045] ③ Add a certain amount of electrophoresis buffer (1X TAE Buffer 100ml).

[0046] ④ Melt completely, cool to about 60°C, add EB5-7ul, and mix well.

[0047] ⑤ Pour the solution into the rubber mold, before inserting the comb in the appropriate position.

[0048] ⑥It solidifies at room temperature. If it is not used immediately, wrap the gel with plastic wrap and store it at 4°C.

[0049] Mix an appropriate amount of PCR product with an appropriate amount of bromophenol blue and add to the gel tank, and add an appropriate amount of DNAMaker to the right tank to start electrophoresis. O...

Embodiment 3

[0055] Embodiment 3: Recombinant expression vector construction

[0056] The selected recombinant expression vector plasmid vector is pBR322, and the construction of the recombinant plasmid includes the following steps:

[0057] (1) Since one of the parents of the pBR322 plasmid is the pMB1 plasmid, at first based on pMB1, the Tn3 translocation of the Rldrd19 plasmid is introduced to obtain the pMB3 plasmid of 13.3kb;

[0058] (2) The molecular weight of pMB3 obviously makes it unsuitable as a carrier, so most of the useless fragments must be lost by digestion under EcoRI active conditions, and the sticky ends of the remaining small fragments of DNA are connected to form pMB8 plasmid (2.6kb);

[0059] (3) At this time, another pSC101 plasmid was digested under EcoRI activity conditions to produce a DNA fragment containing tetr resistance, and this fragment was integrated with pMB8 to form the pMB9 plasmid (5.3kb). At this time, pMB9 is ampstetr phenotype;

[0060] (4) pMB9 ...

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Abstract

The invention provides a facial mask with excellent moisturizing and repairing effects. The facial mask comprises the following components: polypeptide and a humectant, and the polypeptide is a mixedactive protein peptide formed by mixing a small molecular active protein peptide with an amino acid sequence shown as SEQ ID NO: 2 and a small molecular cell-penetrating peptide with an amino acid sequence shown as SEQ ID NO: 3. The TAT-EL mixed active protein peptide is compounded in the formula of the mask to replace tissue / collagen / blood capillaries to accept attack of free radicals, so that the effect of protecting the blood capillaries and collagen is achieved; and the facial mask has excellent effects of promoting cell proliferation and differentiation and accelerating self-renewal and self-repair of skin epidermal cells, and has a good effect on damaged pox muscle / medical beauty wound repair. Acetylated hyaluronic acid is used as one of humectants, so that the solubility, permeability and hyaluronidase resistance of the hyaluronic acid are enhanced; and acetylated hyaluronic acid is amphiphilic, can be dissolved in water and oil, can permeate into a basal layer of skin by takingintercellular substances as carriers, and plays a role in moisturizing.

Description

technical field [0001] The invention relates to a facial mask and a preparation method thereof, in particular to a facial mask with excellent repairing function and a preparation method thereof. Background technique [0002] Peptides are usually added to high-end facial masks. Peptides in facial masks have functions such as cell renewal, metabolism, growth, and repair. Epidermal Growth Factor (EGF) is an important cell growth factor secreted by the human body and has strong physiological activity. However, EGF has not been widely used in the fields of medicine and cosmetics. The reason is that EGF is difficult to achieve in batches. Chemical production: At present, the extraction methods of EGF are mainly divided into two types, one is from animal sources, such as mouse jaw, bovine brain, etc., and the other is from the culture supernatant of stem cells. The content of EGF is very small, and the cost is extremely high. In addition, the molecular weight of the usual EGF is ...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61K8/73A61K8/9789A61K8/9794A61Q17/00A61Q19/00A61Q19/08
CPCA61K8/64A61K8/735A61K8/9794A61K8/9789A61Q19/00A61Q19/08A61Q17/005A61K2800/5922
Inventor 宋筱荭邹弼丞杨丹
Owner 四川省恩乐生物工程有限公司
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