Application of platycladus orientalis

A technology of arborvitae and arborvitae alcohol, which is applied in the field of plant extracts, can solve the problems of inability to develop, poor absorption of free radical scavenging components, inability to function, etc., to achieve the effects of promoting regeneration, perfecting differentiation of cuticle, and delaying the decline of vitality

Pending Publication Date: 2020-01-14
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the fact that some ingredients are difficult to absorb and cannot play their role, simple superposition cannot achieve a multiplied effect, and may even offset them; and external macr

Method used

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  • Application of platycladus orientalis
  • Application of platycladus orientalis
  • Application of platycladus orientalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the production of arborvitae extract

[0033] Take 10 g of Arborvitae branches and leaves, crush them, and extract 2 times at room temperature with 10 times the volume of 70% ethanol 500W ultrasonically for 30 minutes each time. The filtrates are combined, and the filtrates are rotary evaporated to dryness to obtain about 1.3 g of Arborvitae extract.

Embodiment 2

[0034] Embodiment 2: Preparation and testing of skin model

[0035] (1) Preparation: Primary human fibroblasts (NHDF) and primary human keratinocytes (NHEK) were extracted from skin tissue. Resuscitate primary human fibroblasts, culture and digest them and mix them with collagen for 3 to 4 days in the dermis stage, then digest and inoculate the primary human keratinocytes cultured on the feeder layer on the surface of the skin model in the dermal stage, and pass through the epidermis in turn In the immersion culture and gas-liquid phase culture phases, culture to 18 days to obtain a 3D skin model that can be used for detection.

[0036] (2) Test: Use the above-mentioned extracted Thuja arborvitae extract to evaporate to dryness powder, use the culture medium as a solvent to prepare a solution of Thuja arborvitae extract with a final concentration of 10mg / mL, cultivate the skin model for 3 days, add the extract solution according to the test concentration, and Take the model w...

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Abstract

The invention relates to the technical field of plant extracts and discloses application of platycladus orientalis. The invention finds that a natural plant platycladus orientalis extract can significantly up-regulate genes sirt1, nidi1 and igf1 contributing to skin cell and tissue repair and remarkably increase thickness of epidermis to make arrangement of cells on basal layers more closely and differentiation of the cuticle more perfected, which indicates that the platycladus orientalis extract can enhance overall vitality and health conditions of skin, has skin tissue repair capacity, can further continuously promote skin cell proliferation and skin tissue regeneration and delay declination phenomena caused by reduced viability and decreased number of existing skin cells.

Description

technical field [0001] The invention relates to the technical field of plant extracts, in particular to the application of arborvitae. Background technique [0002] The skin covers the entire human body and is in direct contact with the external environment. In addition to protecting the body from various external factors, the skin is also a mirror that reflects the health of the whole body. The human skin is composed of zones, three of which cover the entire human body, the superficial zone (the superficial zone is the epidermis), the dermis, and the deep zone (the deep zone is the subcutaneous tissue). The epidermis is a keratinized, stratified flat epithelium. It consists mainly of keratinocytes and other cells and adheres to the basement membrane that separates the epidermis from the dermis. The dermis is a connective tissue. Its structure derives from the arrangement and interaction between the components of the extracellular matrix and the fibroblasts, which carry o...

Claims

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Application Information

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IPC IPC(8): A61K8/9761A61K36/14A23L33/105A61Q19/08A61P17/18A61P17/16A61K127/00A61K135/00
CPCA61K8/9761A61K36/14A61P17/18A61P17/16A61Q19/08A23L33/105A23V2002/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51A23V2200/318A23V2250/21
Inventor 姚期凤姜丹宋肖洁王越吴越蒋耀权章漳
Owner JALA GROUP CORPORATION
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