A method for raising side-spining bugs
By using defatted cotton balls for hatching, honey water, and mixed juices for feeding during the rearing of the side-necked bug, combined with specific insects and nutrient solutions, the problems of high operational difficulty and insufficient nutrition in existing technologies have been solved, achieving efficient propagation and high egg production of the side-necked bug.
Patent Information
- Application Number
- CN202310317316.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-03-29
- Publication Date
- 2025-11-14
- Estimated Expiration
- 2043-03-29
AI Technical Summary
Existing methods for raising lateral tussock bugs are difficult to implement, requiring constant replacement of corn seedlings. Controlling the number of fall armyworm larvae is challenging, easily leading to food waste and bacterial growth. Fresh leaves are also susceptible to viral infections, resulting in malnutrition.
The eggs of the lateral stinging bug were hatched using defatted cotton balls. They were fed honey water and mixed juices, along with mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae. Nutrient solutions and malic acid were added at different developmental stages, and light conditions were controlled to ensure an adequate food source and nutrient supply.
It simplifies the operation, increases the survival rate and egg production of the side-stinging bug, reduces food waste and the risk of pathogens, provides comprehensive nutritional support, shortens the development period, and extends the survival time of adults.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of insect rearing technology, specifically to a method for rearing the side-necked bug. Background Technology
[0002] fall armyworm Spodoptera frugiperda Fall armyworm (Spodoptera spodoptera), belonging to the order Lepidoptera, family Noctuidae, genus Spodoptera, is a polyphagous pest native to tropical and subtropical America. Widely distributed across the Americas, it damages various crops and is a significant agricultural pest (Todd. EL 1980; Sparks AN, 1979; Martinelli, 2006). Fall armyworm has a strong migratory ability and is a major migratory pest worldwide, primarily damaging over 80 plant species including corn, rice, sorghum, sugarcane, cotton, forage grasses, sugar beets, and potatoes. Fall armyworm larvae mainly damage corn, feeding on leaves and causing defoliation before migrating to other plants. Sometimes, large numbers of larvae damage the roots, severing the stems of seedlings and young plants. Larvae can burrow into the ears of flowering plants, feeding on flower buds and growing points, and even into the fruit. It is characterized by a wide host range, strong reproductive capacity, rapid spread, severe damage, and great difficulty in control (Guo Jingfei et al., 2019). Due to its huge reproductive capacity, outbreak damage, and migratory ability, the fall armyworm will seriously threaten agricultural production and food security.
[0003] The fall armyworm is nocturnal, with its egg stage lasting 3 days at 25°C. Newly hatched larvae feed on the eggshell itself, but prefer to feed on new leaves, typically each finding a specific leaf. The larvae have six instars, and in their final stage, they leave the ink sac, burrow 0.5 cm into the soil, and pupate. The pupal stage lasts approximately 10-12 days. The adult lifespan is about 12 days, and the complete life cycle of the fall armyworm is about 30 days. Its development speed increases with rising temperatures, allowing for several generations per year. A single female moth can lay over 1000 eggs, causing significant damage.
[0004] Taking corn as an example, if 5% of corn seedlings suffer stem breakage and 20% of young plants (within the first 30 days of growth) are damaged, chemical control of the fall armyworm is necessary. For sorghum, the economic threshold for this insect is one (or two) larvae per leaf, or two per ear. In some areas, the insect has developed resistance to pesticides, increasing the difficulty of control. It is evident that chemical control of the fall armyworm is becoming increasingly difficult and its effectiveness is gradually decreasing. Field surveys have revealed that various parasitic wasps in my country can parasitize fall armyworm eggs and larvae, and various predatory natural enemies can prey on its larvae, indicating a promising future for biological control of the fall armyworm.
[0005] The lateral thorn bug (Spodoptera litura), adult, is 12-15 mm long and 6.50-7.50 mm wide, yellowish-brown. It has two black longitudinal stripes on the top of its head, passing through the ocelli. A smooth transverse band without punctures connects the two lateral angles in the middle of the pronotum; the tips of the lateral angles are black, extending outwards. The tip of the scutellum is pale yellowish-white. The anterior margin of the forewing dermestrum forms a broad, straight white edge. The base of the first and second antennal segments is yellowish-brown, the rest black. The legs are pale yellowish-brown. The inventors have found that the lateral thorn bug is effective in controlling the fall armyworm.
[0006] Currently, few domestic scholars have conducted biological and applied ecological research on the side-borne tussock bug. The existing methods for rearing side-borne tussock bugs involve feeding them with corn seedlings, which need to be constantly replaced to support their growth. Their main food source is the fall armyworm. The drawbacks of this method are: 1) The need to constantly replace the corn seedlings increases the difficulty of operation; 2) The fall armyworms used as food also need to be fed with fresh leaves, which is very difficult to operate; 3) The number of fall armyworm larvae needs to be strictly controlled. A large number of fall armyworm larvae can easily lead to food waste and the proliferation of pathogens. In particular, during the rearing period, fresh leaves can easily carry viruses that infect the fall armyworms, causing rapid death and a decrease in the number of fall armyworm larvae, resulting in malnutrition of the side-borne tussock bugs. Summary of the Invention
[0007] In view of the shortcomings of the existing technology, the purpose of this invention is to provide a simple and easily proliferative method for raising the side-necked bug.
[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows: a method for raising lateral thorn bugs, comprising the following steps:
[0009] (1) Place the eggs of the lateral stinging bug and a moist cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs;
[0010] (2) Ensure that the cotton ball contains sufficient moisture and feed it with honey water so that the first instar nymphs can grow into second instar nymphs.
[0011] (3) After the nymphs grow to the second instar, they are fed with mealworms and rice moth larvae, and a nutrient solution for lateral thorn bugs is used to feed them. The nutrient solution for lateral thorn bugs uses 16-18 wt% honey water as a solvent and contains amino acid raw powder at a concentration of 60-65 g / kg, glucose at a concentration of 15-20 g / kg and a mixed juice at a concentration of 200-250 g / kg. The mixed juice is composed of cabbage juice and spinach juice.
[0012] (4) When the nymphs grow to the third instar, feed them with the larvae of the fruit fly and the larvae of the beet armyworm, and supplement them with the nutrient solution for the side thorn bug.
[0013] (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae and beet armyworm larvae, and supplement with nutrient solution for lateral thorn bugs.
[0014] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, feed the adults with mealworms and nutrient solution for side stink bugs.
[0015] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0016] Furthermore, the cotton ball in step (1) is made of defatted cotton; the hatching conditions for the lateral stinging bug eggs are: temperature controlled at 26-28℃, humidity controlled at 65-75%, normal daytime light, supplementary light for 3-5 hours at night, and light intensity controlled at 200-250 Lux during supplementary light.
[0017] Furthermore, the concentration of the honey water in step (2) is 10-15 wt%.
[0018] Furthermore, in step (3), the nutrient solution for the side-spotted bug uses 17-18 wt% honey water as a solvent and contains amino acid raw powder at a concentration of 62-65 g / kg, glucose at a concentration of 16-20 g / kg, and a mixed juice at a concentration of 220-250 g / kg; the mixed juice is made by mixing cabbage juice and spinach juice in any volume ratio.
[0019] Furthermore, in step (3), the ratio of mealworm to rice moth larvae is 3:1.5 to 1.8; the mealworms are 3rd to 4th instar larvae, and the rice moth larvae are 2nd to 3rd instar larvae.
[0020] Furthermore, in step (4), the ratio of oriental fruit fly larvae to beet armyworm larvae is 1:3-5; the beet armyworm larvae are 2-3 instar larvae.
[0021] Furthermore, in step (5), mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae are fed alternately; or mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae are directly mixed and then fed to increase the egg production of the side tussock bug.
[0022] Furthermore, in step (6), the mealworms are 4th to 5th instar larvae.
[0023] Furthermore, in step (6), the nutrient solution for the lateral thorn bug is supplemented with 20-25 g / kg of malic acid.
[0024] Furthermore, the nutrient solution for the lateral crickets in step (6) also contains 22-25 g / kg of malic acid.
[0025] This invention discloses a method for raising the side-spining bug, which utilizes various insects to raise the bug. The raising data are shown in Table 1 below:
[0026] Table 1
[0027]
[0028] In Table 1 above, “----” indicates that the nymphs of the side-spined bug have died and have not laid eggs. This shows that the rearing process of the side-spined bug requires live insects as food to ensure the nymphs' normal survival, growth, and egg-laying. Without live insects, the nymphs will die. This presents a dilemma: feeding too many live insects can lead to food waste and the proliferation of bacteria and maggots, while feeding too few can result in malnutrition. Furthermore, with a monotonous diet, egg production is not ideal, hindering the large-scale propagation of the side-spined bug.
[0029] The beneficial effects of this invention are:
[0030] (1) In view of the problem that the fall armyworm for food needs to be fed with fresh leaves, which makes the operation difficult, this application replaces the fall armyworm with mealworm, rice moth larva, citrus fruit fly larva, and beet armyworm larva, which can be fed with artificial feed; as long as sufficient feed is ensured, the side bug can have a sufficient and bacteria-free food source. The availability of feed is higher than that of fresh leaves, the feeding process is simpler, and the operation is easier.
[0031] (2) In view of the need to strictly control the number of fall armyworm larvae, the large number of fall armyworm larvae can easily lead to food waste and the breeding of pathogens and maggots. In particular, during the rearing period, fresh leaves are prone to carrying viruses that infect fall armyworms, causing fall armyworm larvae to die rapidly and their numbers to decrease, resulting in insufficient nutrition for the side bugs. This application uses a nutrient solution to feed the side bugs. The side bugs use honey water, glucose and mixed juice in the nutrient solution as attractants to attract food. The amino acid powder can provide sufficient nutrients for the side bugs. With the combination of the two, the survival rate of the side bugs is higher and the number of eggs laid is larger, which is beneficial to the large-scale propagation of the side bugs.
[0032] (3) Feeding the lateral tussock bug with a mixture of mealworm, rice moth larvae, citrus fruit fly larvae and beet armyworm larvae can provide more comprehensive nutrition, shorten the development period, increase the survival rate of each development stage, and extend the survival time of the adult bug, which can effectively increase the egg production of the lateral tussock bug.
[0033] This invention provides a method for raising lateral thorn bugs. During the hatching process of lateral thorn bug eggs, light control is also carried out, resulting in a higher hatching rate. In step (6), malic acid is added to the nutrient solution used for lateral thorn bugs to further stimulate their taste and smell, improve their feeding effect, further increase their food intake, activate digestive enzyme activity, promote digestion and absorption, extend the survival time of adult bugs, and effectively increase the number of eggs laid by lateral thorn bugs. Detailed Implementation
[0034] The following embodiments can help those skilled in the art to more fully understand the present invention, but should not be construed as limiting the present invention in any way.
[0035] Example 1
[0036] A method for raising lateral thorn bugs includes the following steps:
[0037] (1) Place the eggs of the lateral stinging bug and a moist defatted cotton ball in the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 26℃, humidity controlled at 65%, normal daytime light, and supplemental light for 3 hours at night. When supplementing light, the light intensity is controlled at 200 Lux.
[0038] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 10wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0039] (3) After the nymphs grow to the second instar, they feed on 3rd-4th instar mealworms and 2nd-3rd instar rice moth larvae. The ratio of mealworms to rice moth larvae is 3:1.5. The second instar nymphs are fed with a nutrient solution for lateral thorn bugs. The amount of mealworms and rice moth larvae fed is slightly less than the amount of lateral thorn bug nymphs consumed. The nutrient solution for lateral thorn bugs uses 16wt% honey water as a solvent and contains amino acid raw powder at a concentration of 60g / kg, glucose at a concentration of 15g / kg, and a mixed juice at a concentration of 200g / kg. The mixed juice is made by mixing cabbage juice and spinach juice in a 1:1 volume ratio.
[0040] (4) When the nymphs grow to the third instar, they feed on the larvae of the oriental fruit fly and the larvae of the second and third instars of the beet armyworm. The ratio of the number of oriental fruit fly larvae to beet armyworm larvae is 1:3. The third instar nymphs are fed with the nutrient solution used for the lateral thorn bug. The amount of larvae fed is slightly less than the amount of food consumed by the lateral thorn bug nymphs.
[0041] (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae, and supplement with nutrient solution for lateral tussock bugs. The amount of larvae fed should be slightly less than the amount of food consumed by the lateral tussock bug nymphs. Specifically, feed them mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae alternately.
[0042] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, feed the adults with 4-5 instar mealworms, and supplement with a nutrient solution for side-sting bugs with 20g / kg malic acid.
[0043] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0044] Example 2
[0045] A method for raising lateral thorn bugs includes the following steps:
[0046] (1) Place the eggs of the lateral stinging bug and a moist defatted cotton ball in the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 28℃, humidity controlled at 75%, normal daytime light, and supplemental light for 5 hours at night. When supplementing light, the light intensity is controlled at 250 Lux.
[0047] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 15wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0048] (3) After the nymphs grow to the second instar, they feed on 3rd-4th instar mealworms and 2nd-3rd instar rice moth larvae. The ratio of mealworms to rice moth larvae is 3:1.8. The second instar nymphs are fed with a nutrient solution for lateral thorn bugs. The amount of mealworms and rice moth larvae fed is slightly less than the amount of lateral thorn bug nymphs consumed. The nutrient solution for lateral thorn bugs uses 18wt% honey water as a solvent and contains amino acid raw powder at a concentration of 65g / kg, glucose at a concentration of 20g / kg, and a mixed juice at a concentration of 250g / kg. The mixed juice is made by mixing cabbage juice and spinach juice in a volume ratio of 1:3.
[0049] (4) When the nymphs grow to the third instar, they feed on the larvae of the oriental fruit fly and the larvae of the second and third instars of the beet armyworm. The ratio of the number of oriental fruit fly larvae to beet armyworm larvae is 1:5. The third instar nymphs are fed with the nutrient solution used for the lateral thorn bug. The amount of larvae fed is slightly less than the amount of food consumed by the lateral thorn bug nymphs.
[0050] (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae, along with nutrient solution for lateral tussock bugs. The amount of larvae fed should be slightly less than the amount of food consumed by lateral tussock bug nymphs. Specifically, mix mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae directly and then feed them.
[0051] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, feed the adults with 4-5 instar mealworms, and supplement with a nutrient solution for side-sting bugs with 25g / kg malic acid.
[0052] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0053] Example 3
[0054] A method for raising lateral thorn bugs includes the following steps:
[0055] (1) Place the eggs of the lateral stinging bug and a moist defatted cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 28℃, humidity controlled at 75%, normal daytime light, and supplemental light for 4 hours at night. When supplementing light, the light intensity is controlled at 220 Lux.
[0056] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 12wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0057] (3) After the nymphs grow to the second instar, they feed on 3rd-4th instar mealworms and 2nd-3rd instar rice moth larvae. The ratio of mealworms to rice moth larvae is 3:1.6. The second instar nymphs are fed with a nutrient solution for lateral thorn bugs. The amount of mealworms and rice moth larvae fed is slightly less than the amount of lateral thorn bug nymphs consumed. The nutrient solution for lateral thorn bugs uses 17wt% honey water as a solvent and contains amino acid raw powder at a concentration of 62g / kg, glucose at a concentration of 16g / kg, and a mixed juice at a concentration of 220g / kg. The mixed juice is made by mixing cabbage juice and spinach juice in a volume ratio of 4:3.
[0058] (4) When the nymphs grow to the third instar, they feed on the larvae of the oriental fruit fly and the larvae of the second and third instars of the beet armyworm. The ratio of the number of oriental fruit fly larvae to beet armyworm larvae is 1:4. The third instar nymphs are fed with the nutrient solution used for the lateral thorn bug. The amount of larvae fed is slightly less than the amount of food consumed by the lateral thorn bug nymphs.
[0059] (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae, along with nutrient solution for lateral tussock bugs. The amount of larvae fed should be slightly less than the amount of food consumed by lateral tussock bug nymphs. Specifically, mix mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae directly and then feed them.
[0060] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, feed the adults with 4-5 instar mealworms, and supplement with a nutrient solution for side-sting bugs with 22g / kg malic acid.
[0061] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0062] Example 4
[0063] A method for raising lateral thorn bugs includes the following steps:
[0064] (1) Place the eggs of the lateral stinging bug and a moist, defatted cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 28℃, humidity controlled at 75%, and normal daytime light.
[0065] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 12wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0066] (3) After the nymphs grow to the second instar, they feed on 3rd-4th instar mealworms and 2nd-3rd instar rice moth larvae. The ratio of mealworms to rice moth larvae is 3:1.6. The second instar nymphs are fed with a nutrient solution for lateral thorn bugs. The amount of mealworms and rice moth larvae fed is slightly less than the amount of lateral thorn bug nymphs consumed. The nutrient solution for lateral thorn bugs uses 17wt% honey water as a solvent and contains amino acid raw powder at a concentration of 62g / kg, glucose at a concentration of 16g / kg, and a mixed juice at a concentration of 220g / kg. The mixed juice is made by mixing cabbage juice and spinach juice in a volume ratio of 4:3.
[0067] (4) When the nymphs grow to the third instar, they feed on the larvae of the oriental fruit fly and the larvae of the second and third instars of the beet armyworm. The ratio of the number of oriental fruit fly larvae to beet armyworm larvae is 1:4. The third instar nymphs are fed with the nutrient solution used for the lateral thorn bug. The amount of larvae fed is slightly less than the amount of food consumed by the lateral thorn bug nymphs.
[0068] (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae, along with nutrient solution for lateral tussock bugs. The amount of larvae fed should be slightly less than the amount of food consumed by lateral tussock bug nymphs. Specifically, mix mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae directly and then feed them.
[0069] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, feed the adults with 4-5 instar mealworms, and supplement with a nutrient solution for side-sting bugs with 22g / kg malic acid.
[0070] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0071] Example 5
[0072] A method for raising lateral thorn bugs includes the following steps:
[0073] (1) Place the eggs of the lateral stinging bug and a moist defatted cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 28℃, humidity controlled at 75%, normal daytime light, and supplemental light for 4 hours at night. When supplementing light, the light intensity is controlled at 220 Lux.
[0074] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 12wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0075] (3) After the nymphs grow to the second instar, they feed on 3rd-4th instar mealworms and 2nd-3rd instar rice moth larvae. The ratio of mealworms to rice moth larvae is 3:1.6. The second instar nymphs are fed with a nutrient solution for lateral thorn bugs. The amount of mealworms and rice moth larvae fed is slightly less than the amount of lateral thorn bug nymphs consumed. The nutrient solution for lateral thorn bugs uses 17wt% honey water as a solvent and contains amino acid raw powder at a concentration of 62g / kg, glucose at a concentration of 16g / kg, and a mixed juice at a concentration of 220g / kg. The mixed juice is made by mixing cabbage juice and spinach juice in a volume ratio of 4:3.
[0076] (4) When the nymphs grow to the third instar, they feed on the larvae of the oriental fruit fly and the larvae of the second and third instars of the beet armyworm. The ratio of the number of oriental fruit fly larvae to beet armyworm larvae is 1:4. The third instar nymphs are fed with the nutrient solution used for the lateral thorn bug. The amount of larvae fed is slightly less than the amount of food consumed by the lateral thorn bug nymphs.
[0077] (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae, along with nutrient solution for lateral tussock bugs. The amount of larvae fed should be slightly less than the amount of food consumed by lateral tussock bug nymphs. Specifically, mix mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae directly and then feed them.
[0078] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, feed the adults with 4-5 instar mealworms and nutrient solution for side stink bugs.
[0079] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0080] Comparative Example 1
[0081] A method for raising lateral thorn bugs includes the following steps:
[0082] (1) Place the eggs of the lateral stinging bug and a moist defatted cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 28℃, humidity controlled at 75%, normal daytime light, and supplemental light for 4 hours at night. When supplementing light, the light intensity is controlled at 220 Lux.
[0083] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 12wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0084] (3) After the nymphs grow to the second instar, they feed on the third- to fourth instar mealworms and the second- to third instar rice moth larvae. The ratio of mealworms to rice moth larvae is 3:1.6. The second instar nymphs are fed with these larvae.
[0085] (4) When the nymphs grow to the third instar, they feed on the larvae of the oriental fruit fly and the larvae of the second and third instars of the beet armyworm. The ratio of the number of oriental fruit fly larvae to the number of beet armyworm larvae is 1:4. The third instar nymphs are fed.
[0086] (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae. The amount of larvae fed should be slightly less than that of the lateral stink bug nymphs. Specifically, mix mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae directly and then feed them.
[0087] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, and feed the adults with 4-5 instar mealworms.
[0088] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0089] Comparative Example 2
[0090] A method for raising lateral thorn bugs includes the following steps:
[0091] (1) Place the eggs of the lateral stinging bug and a moist defatted cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 28℃, humidity controlled at 75%, normal daytime light, and supplemental light for 4 hours at night. When supplementing light, the light intensity is controlled at 220 Lux.
[0092] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 12wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0093] (3) After the nymphs grow to the second instar, they feed on 3rd-4th instar mealworms and 2nd-3rd instar rice moth larvae. The ratio of mealworms to rice moth larvae is 3:1.6. The second instar nymphs are fed with a nutrient solution for lateral thorn bugs. The amount of mealworms and rice moth larvae fed is slightly less than the amount of lateral thorn bug nymphs fed. The nutrient solution for lateral thorn bugs uses 17wt% honey water as a solvent and contains amino acid raw powder at a concentration of 62g / kg.
[0094] (4) When the nymphs grow to the third instar, they feed on the larvae of the oriental fruit fly and the larvae of the second and third instars of the beet armyworm. The ratio of the number of oriental fruit fly larvae to beet armyworm larvae is 1:4. The third instar nymphs are fed with the nutrient solution used for the lateral thorn bug. The amount of larvae fed is slightly less than the amount of food consumed by the lateral thorn bug nymphs.
[0095] (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae, along with nutrient solution for lateral tussock bugs. The amount of larvae fed should be slightly less than the amount of food consumed by lateral tussock bug nymphs. Specifically, mix mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae directly and then feed them.
[0096] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, feed the adults with 4-5 instar mealworms, and supplement with a nutrient solution for side-sting bugs with 22g / kg malic acid.
[0097] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0098] Comparative Example 3
[0099] A method for raising lateral thorn bugs includes the following steps:
[0100] (1) Place the eggs of the lateral stinging bug and a moist defatted cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 28℃, humidity controlled at 75%, normal daytime light, and supplemental light for 4 hours at night. When supplementing light, the light intensity is controlled at 220 Lux.
[0101] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 12wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0102] (3) After the nymphs grow to the second instar, they are fed with the second- to third-instar fall armyworm larvae.
[0103] (4) When the nymphs grow to the third instar, feed them with the larvae of the fall armyworm in the third and fourth instars.
[0104] (5) When the nymphs grow to the fourth instar, feed them with the 4th to 5th instar fall armyworm larvae.
[0105] (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, and feed the adults with 4-5 instar fall armyworm larvae.
[0106] (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
[0107] Comparative Example 4
[0108] A method for raising lateral thorn bugs includes the following steps:
[0109] (1) Place the eggs of the lateral stinging bug and a moist defatted cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; the hatching conditions for the eggs of the lateral stinging bug are: temperature controlled at 28℃, humidity controlled at 75%, normal daytime light, and supplemental light for 4 hours at night. When supplementing light, the light intensity is controlled at 220 Lux.
[0110] (2) Ensure that the cotton ball contains sufficient moisture and feed it with 12wt% honey water so that the first instar nymphs can grow into second instar nymphs.
[0111] (3) After the nymphs grow to the second instar, they are fed with a nutrient solution for lateral thorn bugs. The nutrient solution for lateral thorn bugs uses 17wt% honey water as a solvent and contains amino acid powder at a concentration of 62g / kg, glucose at a concentration of 16g / kg, and a mixed juice at a concentration of 220g / kg. The mixed juice is made by mixing cabbage juice and spinach juice in a volume ratio of 4:3.
[0112] Table 2-3 below presents the statistical results of the rearing experiments of the lateral thorn bugs in Examples 1-5 and Comparative Examples 1-4;
[0113] Table 2
[0114] project Ovulation period / day Nymphal developmental period / d prepupa / d Developmental duration / day Adult egg production / seed Adult survival time / d Example 1 7.5 19.2 8.5 35.2 156.2 20.3 Example 2 7.5 19.3 8.3 35.1 155.6 20.1 Example 3 7.5 19.4 8.9 35.8 156.9 20.5 Example 4 7.5 19.3 8.9 35.7 156.0 19.8 Example 5 7.5 19.7 8.2 35.4 156.2 20.3 Comparative Example 1 7.5 22.1 9.9 39.5 103.6 16.6 Comparative Example 2 7.5 21.4 9.5 38.4 106.7 17.3 Comparative Example 3 7.5 22.1 10.2 39.8 102.2 16.5 Comparative Example 4 7.5 ---- ---- ---- ---- ----
[0115] Table 3
[0116] project Nymphal survival rate / % Survival rate of older nymphs / % Feathering rate / % Example 1 85.5 99.5 99.8 Example 2 85.8 99.6 99.8 Example 3 86.7 99.7 99.9 Example 4 84.8 99.6 99.8 Example 5 85.6 99.0 97.3 Comparative Example 1 75.3 87.2 85.6 Comparative Example 2 79.4 89.1 87.8 Comparative Example 3 73.6 86.5 78.4 Comparative Example 4 ---- ---- ----
[0117] The experimental results above show that the present invention provides a method for raising *Spodoptera litura* (side-necked bugs) by using mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae instead of fall armyworms. Sufficient feed is all that is needed to ensure a sufficient food source for the side-necked bugs. Feed is more readily available than fresh leaves, and the feeding process is simpler and easier to operate. This application also incorporates a nutrient solution for feeding the side-necked bugs. The honey water, glucose, and mixed juice in the nutrient solution act as attractants, while the amino acid powder provides ample nutrients. This combination results in a higher survival rate and increased yield of the side-necked bugs. Larger eggs are beneficial for the large-scale propagation of the lateral tussock bug; feeding the lateral tussock bug with a mixture of mealworm, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae can provide more comprehensive nutrition, shorten the development period, increase the survival rate of each development stage, and extend the survival time of the adults, which can effectively increase the egg production of the lateral tussock bug; light control is also carried out during the hatching process of the lateral tussock bug eggs, resulting in a higher hatching rate; malic acid is added to the nutrient solution used for the lateral tussock bugs in step (6), which further stimulates the taste and smell of the lateral tussock bugs, has a better feeding effect, further increases the amount of food consumed by the lateral tussock bugs, and can activate the activity of digestive enzymes, promote digestion and absorption, extend the survival time of the adults, and effectively increase the egg production of the lateral tussock bugs.
[0118] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.
Claims
1. A method for raising lateral thorn bugs, characterized in that, Includes the following steps: (1) Place the eggs of the lateral stinging bug and a moist cotton ball into the nymph rearing box, and let the eggs of the lateral stinging bug hatch and grow to obtain first-instar nymphs; (2) Ensure that the cotton ball contains sufficient moisture and feed it with honey water so that the first instar nymphs can grow into second instar nymphs. (3) After the nymphs grow to the second instar, they are fed with mealworms and rice moth larvae, and a nutrient solution for lateral thorn bugs is used to feed them. The nutrient solution for lateral thorn bugs uses 16-18 wt% honey water as a solvent and contains amino acid raw powder at a concentration of 60-65 g / kg, glucose at a concentration of 15-20 g / kg and a mixed juice at a concentration of 200-250 g / kg. The mixed juice is composed of cabbage juice and spinach juice. (4) When the nymphs grow to the third instar, feed them with the larvae of the fruit fly citrus and the larvae of the beet armyworm, and supplement them with the nutrient solution for the lateral thorn bug. (5) When the nymphs grow to the fourth instar, feed them with mealworms, rice moth larvae, citrus fruit fly larvae and beet armyworm larvae, and supplement with nutrient solution for lateral thorn bugs. (6) After the fourth instar nymphs grow into adults, set up a light-blocking cloth and an egg-laying device in the nymph rearing box, feed the adults with mealworms and nutrient solution for side stink bugs. (7) Collect the egg masses of the lateral thorn bug; repeat steps (1) to (6) to re-raise the lateral thorn bugs.
2. The method for raising *Spodoptera litura* according to claim 1, characterized in that, The cotton ball in step (1) is made of defatted cotton; the hatching conditions for the lateral stinging bug eggs are: temperature controlled at 26-28℃, humidity controlled at 65-75%, normal daytime light, supplementary light for 3-5 hours at night, and light intensity controlled at 200-250 Lux during the supplementary light period.
3. The method for raising *Spodoptera litura* according to claim 1, characterized in that, The concentration of the honey water in step (2) is 10-15 wt%.
4. The method for raising *Spodoptera litura* according to claim 1, characterized in that, The nutrient solution for the side-spotted bug in step (3) uses 17-18 wt% honey water as a solvent and contains amino acid raw powder at a concentration of 62-65 g / kg, glucose at a concentration of 16-20 g / kg and a mixed juice at a concentration of 220-250 g / kg; the mixed juice is made by mixing cabbage juice and spinach juice in any volume ratio.
5. The method for raising *Spodoptera litura* according to claim 1, characterized in that, In step (3), the ratio of mealworm to rice moth larvae is 3:1.5 to 1.8; the mealworms are 3rd to 4th instar larvae, and the rice moth larvae are 2nd to 3rd instar larvae.
6. The method for raising *Spodoptera litura* according to claim 1, characterized in that, In step (4), the ratio of oriental fruit fly larvae to beet armyworm larvae is 1:3-5; the beet armyworm larvae are 2-3 instar larvae.
7. The method for raising *Spodoptera litura* according to claim 1, characterized in that, In step (5), mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae are fed alternately; or mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae are mixed directly and then fed to increase the egg production of the lateral tussock bug.
8. A method for raising lateral thorn bugs according to claim 1, characterized in that, In step (6), the mealworms are 4th to 5th instar larvae.
9. A method for raising lateral thorn bugs according to claim 1, characterized in that, In step (6), the nutrient solution for the side bugs was also supplemented with 20-25 g / kg of malic acid.
10. A method for raising lateral thorn bugs according to claim 9, characterized in that, In step (6), the nutrient solution for the side bugs was also supplemented with 22-25 g / kg of malic acid.
Citation Information
Patent Citations
Artificial feeding method of Zicrona caerulea
CN107211968A