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Flounder triploid batch producing method

A production method and triploid technology, applied in fish farming, climate change adaptation, application, etc., can solve problems such as difficulty in meeting industrialized production, low triploid induction rate, and inability to apply, and achieve strong practicability. , the effect of neat ploidy and simple operation

Inactive Publication Date: 2011-06-29
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

However, the triploid induction rate obtained in the above research is not high, and it is difficult to meet the needs of industrial production
In addition, so far, there have been no reports on the mass production technology of triploid fry of marine flounder and the growth and gonad development of triploid adult fish in China.
Moreover, due to the differences in species, when the reported technical parameters of triploid induction in freshwater fish are used to induce triploid in marine flounder and flounder, triploid cannot be induced at all, which cannot be applied in practice.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0062] Embodiment 1, batch production of triploid flounder:

[0063] Including three aspects: 1), batch production of triploid brown flounder fry; 2), identification of triploid brown flounder fry; 3), evaluation of growth performance and gonad development status of triploid brown flounder .

[0064] One), batch production of triploid brown flounder fry:

[0065] Its technical content includes: 1) sperm and egg collection and artificial insemination and 2) triploid induction steps.

[0066] 1) Sperm and egg collection and artificial insemination:

[0067] Select the female and male flounder fish that have reached sexual maturity after natural development or artificial induction, and collect eggs and sperm by artificially squeezing the abdomen, and place the collected flounder eggs in a dry beaker at a room temperature of 15-19°C Medium; add the collected fresh semen of flounder into unfertilized eggs, the minimum fertilization volume ratio of semen and eggs is 500-1000μL: 1...

Embodiment 2

[0098] Embodiment 2, mass production of triploid tongue sole

[0099] Including two aspects: 1), batch production of triploid fry; 2), identification of triploid fry; 3), evaluation of triploid growth and gonad development

[0100] One), batch production of triploid fry:

[0101] Its technical content includes: 1) sperm and egg collection and artificial insemination and 2) triploid induction steps.

[0102] 1) Sperm and egg collection and artificial insemination:

[0103] The half-smooth tongue sole broodstock was artificially bred by Shandong Haiyang Huanghai Aquatic Products Co., Ltd. and Laizhou Mingbo Aquatic Products Co., Ltd. The polyploid induction experiment was also carried out in the above two companies. Select female and male adult semi-smooth tongue sole with no damage on the body surface as brood fish. The weight of female fish should be 2-4kg / tail; the weight of male fish should be 200-450g / tail. Breeding, feeding fresh live bait such as shellfish, wild shrimp...

Embodiment 3 3

[0124] Embodiment 3, batch induction of triploid star flounder fry

[0125] Including two aspects: 1), mass induction of triploid flounder fry; 2), identification of triploid flounder fry;

[0126] One), batch induction of triploid fry of spotted flounder;

[0127] Using a screening method similar to the batch induction parameters of triploid fry of brown flounder and tongue sole, the batch induction of triploid fry of spotted flounder was carried out, and the technical conditions suitable for triploid induction of spotted flounder were found out. Obtain triploid fry of spotted flounder. That is, select the mature female and male flounder fish first, collect eggs and sperm by artificially squeezing the abdomen, put the eggs into a dry beaker, and store them at room temperature at 8-9°C; firstly, the Polystat CC series can be used to The cooled constant temperature water bath box was used for different temperature experiments, and the water temperature suitable for cold shock...

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Abstract

The invention relates to a flounder triploid fry batch producing method, which comprises a triploid fry induction method, a method for identifying the triploid produced by the method, and a method for evaluating triploid adult fish growing performance and gonadal development. By the method, a triploid flounder fry batch producing method by adopting low-temperature shock is established, and proper starting time and proper low-temperature treatment time of triploid induction are determined; a method for identifying the triploid fry is established; and the method for evaluating triploid fish growing and gonadal development situation is established. Through the triploid fry induction method, the triploid fry is inducted and generated efficiently in batches, the induction efficiency of the triploid fry is up to 100 percent, the growing speed of the induced triploid fish is 20 percent higher than that of the diploid fish, and the gonad is in diapauses. The method is easy to operate, practical, safe and reliable, has strong practicability, and opens a novel technical method for sex control of paralichthys olivaceus and sole and multiploid breeding.

Description

technical field [0001] The invention belongs to the technical field of aquatic genetics and breeding, and in particular relates to a batch production method of triploid flounder and flounder, that is, the batch production of triploid flounder and flounder through genome operation. Background technique [0002] Flounder and flounder, mainly flounder, half smooth tongue sole, star flounder and turbot, etc., are important marine aquaculture fish in my country. The scale of the breeding industry is getting bigger and bigger, and the output value is getting higher and higher. However, in some flounder and flounder species, gonadal development affects fish growth and meat quality, and the belly bulge caused by gonad development reduces the commercial quality of fish. Therefore, it is very important to carry out research on the sterile technology of these flounder and butterfly fishes and develop sterile populations to improve the aquaculture output and commercial value of these fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 陈松林王磊徐田军刘海金李文龙谢明树杨景峰季相山田永胜
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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