Method for instant relieving diapauses by heat shock and delaying hatching by cold storage for bombyx mori

A technology of heat shock and silkworm, which is applied in the field of biotechnology and bioengineering, can solve the problems that cannot meet the requirements of hatching at any time, and achieve the effect of simplifying the purchase and storage work, simple method, and easy operation

Active Publication Date: 2018-09-04
BEIJING CAU FUTONG GARDENING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low temperature treatment generally requires more than 3 months of low temp...

Method used

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  • Method for instant relieving diapauses by heat shock and delaying hatching by cold storage for bombyx mori
  • Method for instant relieving diapauses by heat shock and delaying hatching by cold storage for bombyx mori
  • Method for instant relieving diapauses by heat shock and delaying hatching by cold storage for bombyx mori

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Embodiment 1

[0035] S1. The eggs of the silkworm diapause variety q-quail silkworm purchased from the Institute of Silkworm Eggs, Chinese Academy of Agricultural Sciences, which had been treated with pickling, were catalyzed in a climate box environment with a temperature of 24-25.5°C and a difference between dryness and humidity of 1.5-2°C. green to hatch and reared to cocoon;

[0036] S2. Collect the male and female silkworm moths that have simultaneously turned into moths under the cocoon protection temperature of 25°C, and then disassemble them after mating for 4-5 hours at 25°C under indoor light conditions, and put the female moths on silkworm paper, and place them in a dark place. Spawn eggs in the environment, spawn for 6-8 hours;

[0037] S3. After laying eggs, the silkworm strips with silkworm eggs are protected in a constant temperature box at 25°C. After 12-24 hours, the silkworm strips are heat-shocked with hot water at 53.1°C for 5 seconds, and diapause is immediately released....

Embodiment 2

[0042] S1. The eggs of the silkworm diapause variety Ze-Tiger silkworm purchased from the Institute of Silkworm Eggs, Chinese Academy of Agricultural Sciences were accelerated to green in a climate box environment with a temperature of 24-25.5°C and a difference between dryness and humidity of 1.5-2°C. Hatch and rear to cocoon;

[0043] S2. Collect the male and female silkworm moths that have simultaneously turned into moths under the cocoon protection temperature of 25°C, and then disassemble them after mating for 4-5 hours at 25°C under indoor light conditions, and put the female moths on silkworm paper, and place them in a dark place. Spawn eggs in the environment, spawn 6-8 hours,;

[0044] S3. After laying eggs, the silkworms are protected in an incubator at 25°C. After 12-24 hours, the silkworm paper is heat-shocked with hot water at 53.1°C for 5 seconds, and diapause is released immediately. After drying, divide into three parts deal with;

[0045] S4. The first batch...

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Abstract

The invention relates to a method for instant relieving diapauses by heat shock and delaying hatching by cold storage for bombyx mori. The method comprises the following steps: S1, incubating silkwormeggs subjected to acid leaching until the silkworm eggs are hatched, and carrying out breeding until cocooning is achieved; S2, collecting male and female silkworm moths which come out at the same time, mating the male and female silkworm moths for 4-5 hours, then separating copulating moth, and putting the female moths on silkworm egg paper for laying eggs; S3, after eggs are laid, protecting silkworm egg paper containing the silkworm eggs into a incubator, carrying out heat shock with hot water for 5 seconds after a preset time, carrying out airing, dividing the silkworm eggs into three parts; and S4, incubating the first part of silkworm eggs, collecting newly hatched silkworms and carrying out breeding; incubating the second part of silkworm eggs, placing the silkworm eggs into a middle storage for 2 hours when the silkworm eggs turn green, wrapping the silkworm eggs with film, placing the silkworm eggs into a cold storage, taking out the silkworm eggs of a preset amount every 3 days, placing the taken silkworm eggs into the middle storage for 2 hours, then placing the silkworm eggs in an environment of 25 DEG C, collecting newly hatched silkworms and carrying out breeding; and placing the third silkworm eggs into the middle storage for 6 hours, then wrapping the silkworm eggs with film in the cold storage, after the silkworm is stored for 30 days, taking a part of the silkworm eggs every 5 days, placing the silkworm eggs into the middle storage for 24 hours, then collecting newly hatched silkworms and carrying out breeding.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioengineering, and relates to a method for immediately relieving diapause of silkworm by heat shock and delaying hatching by cold storage. Background technique [0002] Mulberry silkworm, commonly known as silkworm, is a silk-spinning insect of Lepidoptera with mulberry leaves as food, and has high economic value. Silkworms go through four completely different developmental stages in terms of morphology and physiological function: egg, larva, pupa, and adult, and the different parts and metabolites of each developmental stage have extensive medicinal value. [0003] An important color physiological phenomenon of silkworm is motility, that is, the algebra that occurs in a year. Monomorphic silkworm species only go through one era, and the eggs laid enter a long diapause period until the end of diapause and start to develop under suitable temperature and humidity conditions in the second year; di...

Claims

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Application Information

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IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 苏彦宾张天柱刘鲁江陈燕红傅常智王辉张建盈
Owner BEIJING CAU FUTONG GARDENING
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