Method for instant relieving diapauses by heat shock and delaying hatching by cold storage for bombyx mori

Abstract

The invention relates to a method for instant relieving diapauses by heat shock and delaying hatching by cold storage for bombyx mori. The method comprises the following steps: S1, incubating silkwormeggs subjected to acid leaching until the silkworm eggs are hatched, and carrying out breeding until cocooning is achieved; S2, collecting male and female silkworm moths which come out at the same time, mating the male and female silkworm moths for 4-5 hours, then separating copulating moth, and putting the female moths on silkworm egg paper for laying eggs; S3, after eggs are laid, protecting silkworm egg paper containing the silkworm eggs into a incubator, carrying out heat shock with hot water for 5 seconds after a preset time, carrying out airing, dividing the silkworm eggs into three parts; and S4, incubating the first part of silkworm eggs, collecting newly hatched silkworms and carrying out breeding; incubating the second part of silkworm eggs, placing the silkworm eggs into a middle storage for 2 hours when the silkworm eggs turn green, wrapping the silkworm eggs with film, placing the silkworm eggs into a cold storage, taking out the silkworm eggs of a preset amount every 3 days, placing the taken silkworm eggs into the middle storage for 2 hours, then placing the silkworm eggs in an environment of 25 DEG C, collecting newly hatched silkworms and carrying out breeding; and placing the third silkworm eggs into the middle storage for 6 hours, then wrapping the silkworm eggs with film in the cold storage, after the silkworm is stored for 30 days, taking a part of the silkworm eggs every 5 days, placing the silkworm eggs into the middle storage for 24 hours, then collecting newly hatched silkworms and carrying out breeding.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioengineering, and relates to a method for immediately relieving diapause of silkworm by heat shock and delaying hatching by cold storage. Background technique [0002] Mulberry silkworm, commonly known as silkworm, is a silk-spinning insect of Lepidoptera with mulberry leaves as food, and has high economic value. Silkworms go through four completely different developmental stages in terms of morphology and physiological function: egg, larva, pupa, and adult, and the different parts and metabolites of each developmental stage have extensive medicinal value. [0003] An important color physiological phenomenon of silkworm is motility, that is, the algebra that occurs in a year. Monomorphic silkworm species only go through one era, and the eggs laid enter a long diapause period until the end of diapause and start to develop under suitable temperature and humidity conditions in the second year; di...

AI Technical Summary

Problems solved by technology

Low temperature treatment generally requires more than 3 months of low temperature storage at 5°C, so it cannot meet the requirements of hatching at any time

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