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Method for increasing hatching rate of diapause artemia eggs in weak light environment

A technology of artemia eggs and hatching rate, which is applied in animal husbandry and other fields, can solve the problems of large hatching equipment, non-transparency, and low hatching rate, and achieve the effect of improving hatching rate

Active Publication Date: 2014-02-12
TIANJIN OCEAN PAL CAROL BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual use of the hatchery, the hatching equipment is often very large, and due to the material, it is often not transparent
Hatch rates are often lower than those measured in the laboratory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Artemia eggs hatch without adding any substances under normal conditions

[0026] Use a transparent incubator and a 300ml test tube (250ml seawater in the test tube) for incubation, and the liquid level is 20cm. The hatching density of Artemia eggs is 2g / l

[0027] Incubation conditions of the culture medium:

[0028] Salinity 30g dissolved salt / liter; pH value 8.1; oxygen concentration > 6mg / L; water temperature 28°C,

[0029] The light intensity on the surface of the culture medium was 2800 lux.

[0030] Origin of Artemia eggs

Embodiment 2

[0031] Embodiment 2 directly adds tea polyphenols

[0032] Use a transparent incubator and a 300ml test tube (250ml seawater in the test tube) for incubation, and the liquid level is 20cm. The hatching density of Artemia eggs is 2g / l

[0033] Incubation conditions of the culture medium:

[0034] Salinity 30g dissolved salt / liter; pH value 8.1; oxygen concentration > 6mg / L; water temperature 28°C,

[0035] The light intensity on the surface of the culture medium was 2800 lux.

[0036] Incubation time: 24 hours

[0037] Add tea polyphenols directly to culture medium

[0038] Use of Artemia eggs from Lake Ilavor

[0039] The amount of tea polyphenols (mg / l)

[0040] Using Artemia eggs from Lake Aibi, Xinjiang, China

[0041] The amount of tea polyphenols (mg / l)

[0042] After hatching, observe under a microscope, what is even more surprising is that when the concentration of tea polyphenols reaches 100 mg / l, many Artemia larvae stop hatching after breaki...

Embodiment 3

[0043] Example 3 Add tea polyphenols and use large cultivation equipment instead

[0044] Use a 500L top open, bottom black opaque plexiglass hatch tank for hatching. The hatching density of Artemia eggs is 2g / l

[0045]300L of seawater in the hatching cylinder, the liquid level is about 80cm

[0046] Incubation conditions of the medium: seawater

[0047] Salinity 30g dissolved salt / liter; PH value 8.1; oxygen concentration > 6mg / L; water temperature 28°C,

[0048] The light intensity on the surface of the culture medium was 2800 lux.

[0049] Incubation time: 24 hours

[0050] Encapsulation: Soak the Artemia eggs in the tea polyphenol solution, so that the tea polyphenols are wrapped on the surface of the Artemia eggs.

[0051] Use of Artemia eggs from Lake Ilavor

[0052] The amount of tea polyphenols (mg / l)

[0053] Using Artemia eggs from Lake Aibi, Xinjiang, China

[0054] The amount of tea polyphenols (mg / l)

[0055] Through the comparison of Ex...

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PUM

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Abstract

The invention discloses a method for increasing the hatching rate of diapause artemia eggs in a weak light environment. The diapause artemia eggs are hatched in a hatching culture medium, and the artemia eggs are contacted with a mixture of tea polyphenol and vitamin C sodium; and the concentration ratio of the vitamin C sodium and the tea polyphenol which are introduced into the hatching culture medium is (1:4)-(1:5). The invention aims to provide the method for increasing the hatching rate of the diapause artemia eggs in the weak light environment; and with the adoption of the method, the hatching rate is increased, the stability of the hatching rate is guaranteed, and the stable hatching rate of a large hatching place is guaranteed.

Description

technical field [0001] The invention relates to a method for increasing the hatching rate of diapause Artemia eggs, in particular to a method for effectively improving the hatching rate of diapause Artemia eggs by using polyphenols and VC sodium in a weak light environment. Background technique [0002] Artemia nauplii are generally used as live feed in aquaculture, especially as live feed in the early stage of marine fish and shrimp larvae. There is generally no live feed such as Artemia nauplii on the market, only Artemia eggs, and the nauplii are hatched from Artemia eggs. Hatching of Artemia eggs can be done in hatching medium. Artemia eggs are fully dehydrated and kept in a dry environment with no oxygen and no light at low temperature, and their activity can be preserved for a long time or even several years. Long storage capacity and short time such as 24 hours can produce free-swimming nauplii characteristics, making it the most convenient source of live feed for a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 樊昕宇何家瑞李艳
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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