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Efficient cultivation method of tussah cordyceps militaris

A cultivation method and technology of Cordyceps militaris, which is applied in the field of edible fungus cultivation, can solve the problems of tussah silkworm Cordyceps militaris cultivation mode and the influence of cultivation conditions, restricting the healthy development of tussah silkworm Cordyceps militaris industry, low yield rate of products and low production efficiency, so as to improve product quality. Sloppy production, broad market application prospects, and good product quality

Active Publication Date: 2019-01-01
辽宁省农业科学院大连生物技术研究所
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AI Technical Summary

Problems solved by technology

However, although the artificial cultivation technology of tussah silkworm Cordyceps militaris has been successfully achieved, the cultivation effect of tussah silkworm Cordyceps militaris is greatly affected by tussah silkworm pupae quality, cultivation methods and cultivation conditions, etc. lower
According to Chen Dan (Edible Fungus, 2006(3): 33-34) and Ma Rui (China Animal Husbandry and Veterinary Abstracts, 2013, 9(2): 97-110), the autumn commercial tussah silkworm chrysalis was used as raw material, and conventional glass In the bottle culture method, the yield rate of tussah silkworm Cordyceps militaris is 64% and 65% respectively, and the yield rate and production efficiency of the product are all low, which restricts the healthy development of the tussah silkworm Cordyceps militaris industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Effects of Different Treatment Temperatures on the Quality of Tussah Silkworm Pupa

[0030] 1.1 Test method

[0031] Select the autumn tussah silkworm cocoons of the same variety and in the same stocking conditions that have just been picked and are in the diapause period. The test is divided into 4 groups, and each group randomly selects 1000 tussah silkworm cocoons. Treat under the same conditions for 7 days, then cut the cocoons and take the pupae, investigate the number of diseased pupae and the number of normal pupae, and calculate the incidence rate of tussah silkworm pupae in each test group.

[0032] 1.2 Test results

[0033] The test results carried out according to the above test methods are shown in Table 1.

[0034] Table 1 Effects of different treatment temperatures on the incidence of tussah silkworm chrysalis

[0035] Processing temperature (℃)

[0036] From the test results in Table 1, it can be seen that the incidence rate of tussah silkwor...

Embodiment 2

[0038] Comparison of Weeding Situation of Tussah Silkworm Pupa Cordyceps Militaris Treated by Different Methods

[0039] 1. Test method

[0040] Cordyceps militaris liquid culture medium formula: Potato 200g glucose 20g potassium dihydrogen phosphate 3g magnesium sulfate 1.5g vitamin B 2 10mg peptone 3g beef extract 5g water 1000ml pH value 7.

[0041] Autoclave at 121°C, and keep it for 30 minutes after the pressure reaches 0.1-0.11MPa.

[0042] 2. Preparation of Cordyceps militaris liquid strain

[0043] Take the "Bingyu No. 1" Cordyceps militaris test tube mother seed that was bred and filed at the Dalian Institute of Biotechnology, Liaoning Academy of Agricultural Sciences, and took 0.3cm 2 6 pieces in size, inoculated into 200ml Cordyceps militaris liquid culture medium and cultured statically at 25°C for 2 days, then vibrated at 150rpm / min for 3-5 days, filtered with a 40-mesh screen under sterile conditions to obtain the filtrate, and transferred at a volume ratio o...

Embodiment 3

[0052] Effects of Different Inoculation Sites on the Growth of Tussah Silkworm Cordyceps Militaris

[0053] 1. Test method

[0054] Cordyceps militaris liquid strains were prepared according to the method of Example 1, and the live autumn tussah silkworm pupae treated at 25°C for 7 days were used as materials. The test was divided into 3 groups, and 1000 tussah silkworm pupae were randomly selected in each group, and the inoculation site of the first group was the head of tussah silkworm pupae. About 1cm, the inoculation site of group 2 is the 4th body segment on the back of tussah silkworm chrysalis (insert about 1cm towards the head of the pupae), and the inoculation site of group 3 is about 1cm of tail of tussah silkworm chrysalis, after inoculation, put it into a plastic plate in the culture room Medium cultivation, Cordyceps militaris cultivation management method is the same as 1.3 in example 1, after cultivating for 45 days, analyze and study the growth situation of tus...

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Abstract

The invention discloses an efficient cultivation method of tussah cordyceps militaris. The method comprises the following steps: treating autumn cocoons which are in a diapauses period and are just subjected to cocooning and pupation at a temperature of 19-28 DEG C and humidity of 70-80% for 6-8 days, carrying out storing at 0-4 DEG C for 17-23 days, inoculating 0.3-0.7 ml of a cordyceps militarisliquid strain to the back parts of disinfected tussah living pupae from a third somite to a fifth somite, placing the tussah living pupae into a plastic disc, cultivating cordyceps militaris at proper temperature, humidity and illumination conditions for 45-50 days, and then carrying out harvesting after the cordyceps militaris is mature. Through the cultivation method provided by the invention,the grass yield of the tussah cordyceps militaris reaches 89.2%, the space utilization rate is increased by 56.7%, product quality, production efficiency and economic benefits are remarkably improved,and a technical guarantee is provided for industrialized production of the tussah cordyceps militaris, sufficient raw materials are provided for industries of food, medicine and health-care products,and the market application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a high-efficiency cultivation method of tussah silkworm Cordyceps militaris. Background technique [0002] Cordyceps militaris (cordyCep Smilitaris (L.) Link), also known as Cordyceps militaris, is a model species of the genus Cordyceps of the family Ascomycotinae Ergotaceae. Dual-purpose fungi, the Ministry of Health listed it as a new resource food in 2009. It has the effects of improving the body's immunity, anti-tumor and improving myocardial function. [0003] Tussah Cordyceps militaris is the wild Cordyceps militaris separated and domesticated, then inoculated into live tussah silkworm pupae to cultivate the fruiting body. , which not only contains rich nutrition, but also contains active ingredients such as cordycepin, cordycepic acid and cordyceps polysaccharide. Among them, cordycepin is a unique nucleoside active substance in Cordyceps s...

Claims

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Application Information

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IPC IPC(8): A01G18/00A01G18/20A01G18/40
CPCA01G18/00A01G18/20A01G18/40
Inventor 都兴范孟楠李亚洁温志新李学军米锐马淑慧孙永欣李树英王晓燕
Owner 辽宁省农业科学院大连生物技术研究所
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