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Raman spectrum model establishment method for rapid nondestructive identification on diapause termination silkworm eggs

A technology of Raman spectrum and construction method, which is applied in the field of Raman spectrum model construction for rapid and non-destructive identification and release of diapause silkworm eggs, which can solve the problem of difficulty in distinguishing diapause eggs from diapause eggs, silkworm eggs can no longer hatch silkworms, Problems such as the distinction of silkworm diapause have not been found, and the effect of reducing the requirement for technical proficiency has been achieved

Active Publication Date: 2018-11-13
SOUTH CHINA AGRI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are obvious differences in material composition and composition ratio between diapause eggs and released diapause eggs, but the appearance of diapause eggs and released diapause eggs is difficult to distinguish with the naked eye. At present, the method of silkworm egg embryo dissection is mainly used in production, combined with Judgment can be made by observing with a microscope, but the dissected silkworm eggs cannot be hatched and raised again, and technical personnel are required to be skilled in operation, master the key technical essentials of embryo anatomy, and have good eyesight
[0006] In the current silkworm industry, no one has been found to apply Raman spectroscopy detection technology to the distinction of silkworm diapause

Method used

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  • Raman spectrum model establishment method for rapid nondestructive identification on diapause termination silkworm eggs
  • Raman spectrum model establishment method for rapid nondestructive identification on diapause termination silkworm eggs
  • Raman spectrum model establishment method for rapid nondestructive identification on diapause termination silkworm eggs

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment 1 Construction of silkworm egg Raman spectrum model

[0041] 1. Acquisition of Raman spectral data: the laser wavelength of the Raman spectrometer is set to 785nm, and the laser power is 40mw; using the continuous detection function of FinderOne, the center wavelength is set to 1400cm -1 ; The minimum wavenumber of spectral detection is 400cm -1 , the highest wave number is 1800cm -1 ; The integration time is 10 seconds, and the number of accumulations is 1 time; a quartz microscope lens is used for focusing; the measurement room temperature is 20°C. For each detection, the incident light is aimed at the middle of the silkworm egg, the focal length is adjusted to obtain a clear image, and then the Raman spectral data in the format of "wavenumber-spectral intensity" is collected. figure 2 It is the Raman spectrum of silkworm egg samples of Dazao and 9fu×7xiang varieties diapausing and releasing diapause, in which (a) is diapause of Dazao, (b) is diapause of...

Embodiment 2

[0048] Analysis of silkworm diapause based on the regression coefficient matrix of the PLS_DA algorithm: According to the principle of PLS_DA, in the regression coefficient matrix, the larger the coefficient corresponding to the wave number, the greater the importance of the wave number in the modeling. Combined with the distribution characteristics of spectral data, the characteristic peaks of the Raman spectrum of silkworm eggs can be identified. The operation is as follows: After removing the fluorescence, calculate the difference between the mean values ​​of the Raman spectra of diapause silkworm eggs and released diapause silkworm eggs, and then multiply it by the corresponding regression coefficient matrix B to obtain the two kinds of silkworm egg spectra in the PLS_DA model. Mathematical distance ΔY, that is: ΔY=B×(X 滞育 -X 解除滞育 ).

[0049] image 3 It is a comparison chart of the processing effect before and after the window moving polynomial least squares smoothing ...

Embodiment 3

[0051] Example 3 Evaluation of the performance of the silkworm egg Raman spectrum model

[0052] Use the confusion matrix and its derivative indicators and the receiver operating characteristic curve to define the quality of the model. Confusion Matrix is ​​a common way to show the prediction accuracy of the model. It is a N*N contingency table (N is the number of categories of the classification). Taking the binary classification as an example, the confusion matrix of the classification results is shown in Table 1 below.

[0053] Table 1 Confusion Matrix

[0054]

[0055] According to the four basic indicators in Table 1, multiple classifier evaluation indicators can be derived, and the commonly used indicators are as follows:

[0056] Accuracy: It is aimed at the correct number of all recognitions in the test set. The calculation formula is: accuracy=(TP+TN) / (TP+TN+FN+FP).

[0057] True positive rate (true positive rate, TPR), which shows how much the classification ...

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Abstract

The invention belongs to the technical field of Raman spectrum detection and discloses a Raman spectrum model establishment method for rapid nondestructive identification on diapause termination silkworm eggs. The method comprises a Raman spectrum detection technique and a PLS_DA classification algorithm and mainly comprises steps of sample selection, data acquisition, data digging, conclusion analysis, and the like, wherein the step of sample selection comprises steps of selecting diapause eggs and diapause termination eggs of two varieties of Dazao and 9Fu*7Xiang; the step of data acquisition comprises steps of carrying out spectrum data acquisition by using a laser Raman spectrometer, processing Raman spectrum data, and establishing a PLS_DA qualitative analysis Raman spectrum model. Byadopting the method, diapause and diapause termination silkworm eggs can be rapidly and nondestructively distinguished, and identification analysis on nondestructive diapause termination household silkworm eggs can be finally achieved.

Description

technical field [0001] The invention belongs to the technical field of Raman spectrum detection, and more specifically relates to a method for constructing a Raman spectrum model for rapid and non-destructive identification and release of diapause silkworm eggs. Background technique [0002] Raman spectroscopy is an analysis method based on molecular vibration and rotation information and applied to molecular structure research. For the chemical groups of various substances, the Raman spectrum has sharp characteristic peaks, and the position and strength of the peaks can sensitively reflect the structure and change information of the relevant substances. Therefore, Raman spectroscopy is an effective method for qualitative identification and quantitative analysis of substances. [0003] The material composition and composition ratio of diapausing silkworm eggs are very different from those of silkworm eggs released from diapause. The main substances of silkworm eggs are 65%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 代芬王叶元钟仰进彭斯冉孙鹏鹏黄袁云梁春晖
Owner SOUTH CHINA AGRI UNIV
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