Method for producing cultivated silkworm novel green cocoon practical variety by using GFP protein

A silkworm and variety technology, applied in the fields of bioengineering and biotechnology, can solve problems such as no research reports, and achieve the effect of simplifying the spinning process, unique optical characteristics, and wide use.

Inactive Publication Date: 2009-05-06
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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Embodiment 1

[0028] The first step: use the practical silkworm variety "Bibo" (the original species of Japanese white cocoon) as the raw material, remove the diapause of the first-laid eggs, and use an Eppendorf microinjector to inject 10- 15n1, a total concentration of 400ng / u1 recombinant piggyBac vector and helper vector with red fluorescent protein tagged by nerve and eye-specific promoters and green fluorescent protein (GFP protein) promoted by silk fibroin heavy chain promoter, co-injected into 1500 In the silkworm eggs, the injection port was sealed with non-toxic glue, and after being sterilized in 35% formaldehyde vapor for 5 minutes, they were placed in a high-humidity environment at 25°C and a relative humidity of more than 85% to accelerate the hatching, and the hatched 73 heads of G0 The generation ant silkworms were collected and raised with artificial feed until they became moths, and 40 G0 generation silkworm moths were obtained. A total of 27 G1 generation silkworm eggs wer...

Embodiment 2

[0032] The first step: using the practical silkworm variety "872" (the original species of Japanese white cocoon) as the raw material, the newly laid eggs were released from diapause, and 3-6 hours after laying, the 10- 15nl, total concentration of 400ng / ul recombinant piggyBac vector and helper vector with red fluorescent protein tagged by nerve and eye-specific promoter and green fluorescent protein (GFP protein) promoted by silk fibroin heavy chain promoter, co-injected into 2500 In the silkworm eggs, seal the injection port with non-toxic glue, sterilize in 35% formaldehyde vapor for 5 minutes, and place them in a high-humidity environment at 25°C and a relative humidity above 85% to accelerate the hatching. The hatched 163 G0 The generation ant silkworms were collected and raised with artificial feed until they became moths, and 121 G0 generation silkworm moths were obtained. A total of 63 G1 generation silkworm eggs were obtained through selfing or backcrossing, and 63 G1...

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Abstract

The invention relates to a method for creating a novel green cocoon practical variety of silkworms by using GFP protein. The method comprises the step of fusing large amount of the GFP protein in chromoproteins expressing and deciding colors of related marine organisms such as coral reefs and so on in silkworm fibroin by using a transgenic technology for the silkworms, in particular a transgenic technology for a diapause variety, so as to quicken cultivating the novel green cocoon practical variety of the silkworms. The method has the advantages that the method not only quickens practical process of transgenic silkworms greatly, but also produces fiber fabrics capable of being directly applied to weaving silk without being printed and dyed, simplifies spinning technology greatly, saves production cost and is favorable for environmental protection.

Description

technical field [0001] The invention relates to the fields of biotechnology and bioengineering, in particular to a method for creating a new type of green cocoon practical variety of silkworm by using GFP. Background technique [0002] Natural colored cocoons are not only bright in color, but also have antibacterial and health care effects on the skin. Natural colored cocoon silk does not need chemical dyeing if it is properly processed, and can avoid environmental pollution. It is a green and environmentally friendly raw material. At present, natural colored cocoon silkworm varieties mainly include two categories: yellow-red cocoon and green cocoon: 1. Yellow-red cocoon: silkworms of the yellow-red cocoon are regulated by the yellow cocoon gene during growth and development, and eat mulberry leaves. Carotenoids (β-carotene, nascent β-carotene) and lutein pigments (lutein, dandelion flavin, purple flavin, hypoxanthine) and other pigments are absorbed and transformed Import...

Claims

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Application Information

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IPC IPC(8): C12N15/62C12N15/63C12N15/89A01K67/04
Inventor 夏庆友赵爱春徐汉福周泽扬鲁成向仲怀
Owner SOUTHWEST UNIVERSITY
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