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Method for regulating and controlling diapause of agryponflexorius

A technology for the diapause and grassland, which is applied in the field of regulating the diapause of the meadow moth, can solve the problems of restricting the large-scale application of natural enemy insects, the lack of diapause control technology, and the extension of the storage period, so as to promote large-scale Multiply, improve the efficiency of pest control, prolong the shelf life of products and the effect of

Inactive Publication Date: 2013-06-12
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the traditional method of continuous reproduction, the bee source is easy to degrade, and it is not easy to preserve for a long time, which brings great difficulties to the release in the field; by improving the breeding technology and artificially inducing the bee to enter the diapause state, the storage period can be greatly extended. , there is no diapause control technology, which often causes the natural enemy insects to die before the pests occur, or the natural enemy insect products have not been produced when the pests occur. bottleneck factor

Method used

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  • Method for regulating and controlling diapause of agryponflexorius
  • Method for regulating and controlling diapause of agryponflexorius
  • Method for regulating and controlling diapause of agryponflexorius

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the induction of diapause of the meadow borer Agia japonica

[0039] 1. Determination of the diapause temperature and photoperiod of the meadow moth Agia

[0040]Using the two-factor orthogonal test method, set 5 different photoperiods (L8:D16, L10:D14, L12:D12, L14:D10, L16:D8) and 4 different temperatures (17°C, 20°C, 23°C, 26°C), a total of 20 treatments, 30 meadow borer larvae (hosts) for each treatment, repeated 3 times, and the meadow moth larvae were parasitized by the meadow borer larvae after mating for 1 day, and then transferred to sandy soil Put them into the artificial climate box with different photoperiod and temperature treatment (among them, the light intensity during the light is 4000-5000LX, and the relative air humidity is 70%-80%). Carry out diapause induction (diapause induction is regarded as starting from the stage of "egg"), and the induction time is 2 days. After induction, it is transferred to the normal feeding conditions of th...

Embodiment 2

[0054] Embodiment 2, the maintenance of diapause of the meadow borer Agia

[0055] In this example, the time for low-temperature storage of the diapause cocoons of the meadowborer Agna was determined by measuring the eclosion rate of the diapause cocoon of the meadowborer, and the lifespan, oviposition period, and parasitism rate of the adult bees after emergence. details as follows:

[0056] 1. The eclosion rate of the diapause cocoons of the meadow moth A.

[0057] The diapause state of the diapause state of the meadow moth Agna (the appearance is a cocoon at this time) obtained by the method of Example 1 is collected in moist and soft soil, and packed into plastic bowls (diameter 10cm, height 8cm), plastic The bowl is covered with breathable gauze, stored in a refrigerator at 4°C in total darkness, sprayed with water every 3 days, and maintained at a relative humidity of 60%-70%. The diapause cocoons were regularly taken out from the refrigerator and placed in an incubato...

Embodiment 3

[0066] Embodiment 3, the diapause of the meadow moth Aguisia japonica is removed

[0067] For adopting the method of embodiment 1 to obtain the diapause state meadow moth Agna (diapause cocoon), the diapause state can be released through temperature stimulation, and enter the normal development process, the specific measures are as follows:

[0068] Place the diapause cocoons of the meadow moth A. japonica in an environment with a temperature of 23°C, a photoperiod of L14:D10 to L16:D8, and a light intensity of 4000-5000Lx. Water is sprayed every 3 days to maintain a certain humidity (RH is 70% -80%), the diapause state can be released after 20 days, and the diapause cocoons will all emerge into adult bees soon.

[0069] Among them, the induction time of 20 days is determined according to the following method:

[0070] The diapause cocoons of the meadowlark moth A. japonica were transferred to the incubator with a temperature of 23°C and a photoperiod of L14:D10 (RH is 70%-80...

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Abstract

The invention discloses a method for regulating and controlling agryponflexorius, which adopts following steps: 1), agryponflexorius to be subjected to diapause induction is placed under the condition with the temperature of 17 plus / minus 1 DEG C and a photoperiod from (L8 : D16) to (L12 : D12) for induction, and is enabled to enter a diapause state; 2), the agryponflexorius entering the diapause state in the step 1 is stored under a dark condition with the temperature of 4 DEG C; and 3), after being ended in storage in step 2, the agryponflexorius resumes growth under a condition with temperature of 23 DEG C and a photoperiod from L14 to D10 to L16 to D8. The method has the advantages that the diapause induction rate is 100%, the storage period after diapause is 120 days, and after 20 days from diapause removal, adult agryponflexorius can be hatched. According to the invention, the agryponflexorius can be propagated in large, the shelf life of products and time in keeping prevention and control functions in fields can be prolonged, the prevention and treatment efficiency of insects is improved, and the method has important significance in knowing on developmental mechanism of natural enemy insects and exploring adaptive mechanism of insects to environment and evolutionary approaches of insects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for regulating the diapause of the meadow moth Agna. Background technique [0002] In the grassland ecosystem, biological communities exist in the form of multiple biological populations, and they are linked together by the relationship of food chains. Any change in any link will inevitably cause changes in other links. Therefore, within a certain range of grassland ecosystems, analyzing the relationship between pest populations and natural enemy populations, and artificially strengthening the power of natural enemy populations to control pest populations, can reduce the number of pest populations to a level that cannot cause harm to pastures. The use of natural enemy insects to control pests is the most widely used method in biological control. [0003] The meadow moth Loxostege sticticalis Linnaeus (Lepidoptera: Mothidae) is a sudden polyphagous migratory pest in agricultur...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 刘爱萍徐林波高书晶张礼生徐忠宝刘雅学缪丽梅康爱囯崔智林特木儿
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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