Method for inducing copepoda to produce diapause eggs
A technology for diapausing eggs and copepods, which is applied in the field of aquaculture, can solve problems such as unsatisfactory, difficult to separate viruses and bacteria, and inability to guarantee the quantity, stable quality and timely supply of copepods, and achieve the effect of a convenient method
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[0032] The following will be further described in conjunction with the accompanying drawings and specific embodiments, but it is not intended to further limit the present invention. In implementation process, the present invention can adopt following specific steps:
[0033] 1. Algae culture
[0034] Microalgae (Isochrysis globosa and Prorocentrum marine) were inoculated in f / 2 culture medium, salinity 27±1, photoperiod 12L︰12D, room temperature (22~24℃) and cultured until logarithmic growth expected use.
[0035] 2. Seawater treatment
[0036] The seawater used in the present invention is all natural seawater, sieved with 20 μm silk to remove floating sand and sundries, sterilized at high temperature and continuously inflated for 24 hours, and then adjusted to a salinity of 27±1.
[0037] 3. Cultivation of Daphnia pacificus
[0038] In the present invention, Daphnia pacificis is sampled in Xiamen sea area, and successfully purified and cultivated in a laboratory. Two gen...
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