Method for adjusting diapause of Exorista civilis Rond

A technology of umbrella skirt chasing parasitic flies and diapause, which is applied in animal husbandry and other fields, can solve problems such as difficult long-term storage, difficulty in field release, and easy degradation of flies, so as to improve control efficiency, prolong control time, and promote large-scale multiplication effect

Inactive Publication Date: 2014-04-09
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the traditional method of continuous reproduction, the flies are easy to degenerate, and it is not easy to preserve for a long time, which brings great difficulties to the release in the field.

Method used

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  • Method for adjusting diapause of Exorista civilis Rond
  • Method for adjusting diapause of Exorista civilis Rond
  • Method for adjusting diapause of Exorista civilis Rond

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the diapause induction of umbrella skirt chasing fly

[0039] 1. Determination of diapause temperature and photoperiod of umbrella skirt chasing fly

[0040]Set 4 different temperatures (14°C, 17°C, 21°C and 25°C) and 5 different photoperiods (L8:D16, L10:D14, L12:D12, L14:D10, L16:D8), a total of 20 treatments , for each treatment, 30 healthy mesophila larvae (hosts) from the end of the 4th instar to the beginning of the 5th instar were repeated 3 times. Put the female fly of the umbrella skirt-chasing fly and the larvae of the grass moth 1 day after mating together in the insect culture box, and put them into the artificial climate box with different photoperiods and temperature treatments (wherein, the light intensity during the light is 4000~5000LX, The relative air humidity is 60%-70%), and the diapause induction is carried out on the umbrella skirt fly (diapause induction starts from the stage of "egg"), and the induction is 10-12 days. After the i...

Embodiment 2

[0057] Embodiment 2, the diapause maintenance of umbrella skirt chasing fly

[0058] 1. Eclosion rate of umbrella skirt-chasing flies

[0059] The diapause larvae of the diapause state obtained by the method of Example 1 (parasitic in the pupated Meadowsia moth) were placed in a plastic container filled with moist fine sand, and then put into the refrigerator for 4 ± Store in total darkness at 2°C, spray water every day to keep the relative air humidity at 70%. Regularly take out the meadow moth cocoons parasitized with diapause larvae larvae from the refrigerator and put them in an incubator with a temperature of 23±1°C and a photoperiod of L16:D8 for the eclosion of the diapause fly, observe and make statistics Eclosion rate of parasitic flies after different periods of refrigeration. In the experiment, 8 treatments (8 cold storage times, see Table 3) were set up, and each treatment had 50-70 meadow moth cocoons parasitized with diapause larval parasitoid fly larvae. At t...

Embodiment 3

[0067] Embodiment 3, the diapause removal of umbrella skirt chasing fly

[0068] For the umbrella skirt chasing flies of the diapause state obtained by the method of embodiment 1, the diapause state can be removed through temperature stimulation, and the normal development process can be entered, and the specific measures are as follows:

[0069] Put the meadow moth cocoons parasitized with diapause larvae larvae in an environment of 23°C, photoperiod L14:D10, and light intensity 5000Lx, spray water once a day, and keep a certain humidity (RH is 70%), 20 Days later, the umbrella skirt chasing fly larvae can be released from the diapause state, and all of them will emerge into adult flies soon.

[0070] Among them, the induction time of 20 days is determined according to the following method:

[0071] The meadow moth pupae parasitized with diapause shed skirt chasing fly larvae after being stored for 60 days in total darkness at 4°C by the method of Example 2 were transferred ...

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Abstract

The invention discloses a method for adjusting the diapause of Exorista civilis Rond. The method comprises the following steps that (1) the Exorista civilis Rond with the diapause to be induced is placed in the environment with the conditions that the temperature is 17+/-1DEG C, and the photoperiod ranges from L8:D16 to L12:D12, and induction is carried out on the Exorista civilis Rond so that the Exorista civilis Rond can be in a diapause state; (2) the Exorista civilis Rond which is obtained in the step (1), and is in the diapause state is stored in the dark with the temperature of 4DEG C; (3) after storage in the step (2) is accomplished, the Exorista civilis Rond is placed in the environment with the conditions that the temperature is 23+/-1DEG C, and the photoperiod ranges from L14:D10 to L16:D8, and development is recovered. According to the method for adjusting the diapause of the Exorista civilis Rond, the diapause induction rate is 100%, the storage period after the diapause can be 120 days, and eclosion adult Exorista civilis Rond can be obtained after 20 days when the diapause is removed. The method for adjusting the diapause of the Exorista civilis Rond has important significance for large-scale expanding propagation of the Exorista civilis Rond, extension of the shelf life of Exorista civilis Rond products and the field prevention and control action time of the Exorista civilis Rond products, improvement of pest control efficiency, deepening of understanding about the natural enemy insect development mechanism, and exploration of the adaptive mechanism of insects to the environment and the evolution path of the insects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for regulating the diapause of parasitoid flies. Background technique [0002] Natural enemy insects are insects that parasitize or prey on other insects, and they control the development and spread of pests in farmland, forest areas and pastures. The resources of natural enemy insects in our country are very rich and have a strong material basis for development and application. By developing the large-scale production technology of natural enemy insects, adopting the method of large-scale breeding of natural enemy insects indoors and the technology of large-scale release, the initial population of natural enemies in the field can be increased, combined with biological pesticides and ecological technologies, a relatively complete technical system of pest control can be formed to replace or reduce The frequency and dosage of chemical pesticides are used to protect the environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 刘爱萍王育青徐林波高书晶刘雅学王建梅缪丽梅王宁夏明康爱囯
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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