Method for regulating and controlling diapause of microplitis syntretomorpha

A technology of diapause and wasp, applied in animal husbandry and other directions, to achieve the effect of improving the quality of agricultural products, prolonging the preservation period, ecological and economic benefits.

Inactive Publication Date: 2014-09-17
HUNAN AGRICULTURAL UNIV
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  • Claims
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Problems solved by technology

However, natural enemy products against Spodoptera litura,

Method used

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  • Method for regulating and controlling diapause of microplitis syntretomorpha
  • Method for regulating and controlling diapause of microplitis syntretomorpha

Examples

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Embodiment 1

[0019] 1) Acquisition of diapause signal worm state: including three processes of host breeding, bee breeding, and bee inoculation.

[0020] Host culture: In an artificial climate chamber (27±2°C, L14:D10, relative humidity 70±10%), Spodoptera litura is reared from generation to generation. The larvae were fed with artificial feed, and after reaching the sixth instar mature larvae, the larvae were transferred to flat-bottomed test tubes and raised separately until pupation. The adults were fed with 10% honey water after eclosion, and the male and female were paired to collect eggs, and the egg masses were transferred to the artificial feed to be hatched.

[0021] Breeding of bees: Spodoptera litura of 2-3 ages is used as a host, and Spodoptera litura and vegetable leaves are moved into a flat-bottomed test tube, and cotton balls dipped in 30% honey water are placed at the bottom of the test tube. According to the ratio of bee to host: 1: The ratio of 10 was inserted into the ...

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Abstract

The invention provides a method for regulating and controlling diapause of microplitis syntretomorpha. The method includes the steps of diapause induction, diapause storage and diapause relieving. In the step of diapause induction, prodenia litura where microplitis syntretomorpha is parasitized for 1 day to 3 days is independently subpackaged in flat-bottom test tubes, the test tubes are placed under the condition with the temperature of 18+/-1 DEG C, the relative humidity of 70+/-10% and the photoperiod of L10:D14 for induction, and microplitis cocoons in the diapause state are obtained after host larvae come out of the cocoons. In the diapause storage step, wet fine sand is laid on the diapause cocoons after the host larvae are removed, the diapause cocoons are transferred to the dark environment with the temperature of 6 DEG C to be stored. In the diapause relieving step, the diapause cocoons are placed under the condition with the temperature of 27+/-2 DEG C, the humidity of 70+/-10% and the photoperiod of L14:D10, and eclosion microplitis syntretomorpha is obtained in about 5 days. The diapause rate of microplitis syntretomorpha induced through the method can reach more than 98%, the diapause cocoons are stored for 3 months to 5 months under the condition of 6 DEG C, and the eclosion microplitis syntretomorpha does not degenerate in the aspects of the egg laying amount, the life and other biological indicators. By means of the method, the retention cycle of microplitis syntretomorpha can be effectively prolonged by 4 times to 5 times, and the reference basis is provided for regulating and controlling development of microplitis syntretomorpha.

Description

technical field [0001] The invention belongs to the technical field of biological control of agricultural pest control, and relates to a method for regulating the diapause of the scutellaria schneidi. Background technique [0002] Spodoptera litura is a worldwide distribution of omnivorous and explosive agricultural pests, which can harm 99 families and more than 290 species of plants, with serious generation overlap. The hatching larvae are gluttonous and will wipe out the flowers, leaves, fruits, and even stems of ground crops. There are many hosts and many generations in a year, which makes the damage of Spodoptera litura increase year by year. A large number of frequent use has made the resistance of Spodoptera litura increased year by year, and it cannot be effectively controlled. Therefore, there is an urgent need to improve and enhance the pest control technology in production, and the use of biological control to prevent and control pests can well avoid the shortcom...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 黄国华张云宣徐芝陈旭阳李顺姬刘双清
Owner HUNAN AGRICULTURAL UNIV
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