The invention discloses a novel rapid chick
embryo egg sex identification method which comprises the following steps:
hatching an egg to 16, 17 or 18 days of age, aseptically obtaining
allantoic fluid (10 microliters) from the tip of the
embryo egg through a 1 ml of
injector, sampling and sealing with
paraffin wax for normal
hatching; taking the obtained
allantoic fluid as a template, designing a CDH-W specific primer for PCR amplification, detecting the amplified primer through
gel electrophoresis and performing sex identification, wherein double bands (600 bp and 450 bp) are adopted for male amplification, and a
single band (450 bp) is adopted for female amplification; after normal
hatching, statistically calculating the hatching rate and the death rate, and performing biostatistical analysis with a control group; comparing a sex identification result with an autopsy result of a hatched chick, performing
correlation analysis, and statistically calculating the accuracy rate of sex identification (if reaching 99 percent, the accuracy rate is deemed to be 100 percent of effective).