The invention discloses a novel rapid chick 
embryo egg sex identification method which comprises the following steps: 
hatching an egg to 16, 17 or 18 days of age, aseptically obtaining 
allantoic fluid (10 microliters) from the tip of the 
embryo egg through a 1 ml of 
injector, sampling and sealing with 
paraffin wax for normal 
hatching; taking the obtained 
allantoic fluid as a template, designing a CDH-W specific primer for PCR amplification, detecting the amplified primer through 
gel electrophoresis and performing sex identification, wherein double bands (600 bp and 450 bp) are adopted for male amplification, and a 
single band (450 bp) is adopted for female amplification; after normal 
hatching, statistically calculating the hatching rate and the death rate, and performing biostatistical analysis with a control group; comparing a sex identification result with an autopsy result of a hatched chick, performing 
correlation analysis, and statistically calculating the accuracy rate of sex identification (if reaching 99 percent, the accuracy rate is deemed to be 100 percent of effective).