Serum protein fingerprint spectrum method for nasopharyngeal carcinoma detection

A fingerprint and nasopharyngeal cancer technology, applied in the field of medical detection, can solve the problems of limited application, difficult to achieve ideal repeatability, high price of electrophoretic gel, etc., achieve high sensitivity and specificity, reasonable and feasible design, and improve the cure rate Effect

Inactive Publication Date: 2006-10-04
ZHUHAI BAIYUAN BIOLOGICAL INFORMATION TECH
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AI Technical Summary

Problems solved by technology

The traditional classic technology of proteomics - two-dimensional electrophoresis, although it can analyze the composition of serum protein, but only one serum sample can be analyzed at a time, the price of electrophoresis gel is high, the serum needs to be processed complicatedly, the analysis is time-consuming, and it is difficult to analyze For proteins with a molecular weight below 20,000 Daltons, because the electrophoresis results are greatly affected by sample processing and operating conditions, it is difficult to achieve ideal repeatability, it is difficult to establish an effective large-scale database, and it is impossible to enter clinical applications from the laboratory
Another traditional proteomics technology, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, cannot directly analyze natural protein samples, and needs to be used in conjunction with in situ protein digestion technology, high performance liquid chromatography or capillary electrophoresis, which limits its application

Method used

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  • Serum protein fingerprint spectrum method for nasopharyngeal carcinoma detection
  • Serum protein fingerprint spectrum method for nasopharyngeal carcinoma detection
  • Serum protein fingerprint spectrum method for nasopharyngeal carcinoma detection

Examples

Experimental program
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Effect test

Embodiment approach 1

[0028] Sample collection: The patient to be tested had chronic nasopharyngitis, and the Epstein-Barr virus antibody test was positive, and CT showed changes in the right nasopharyngeal lateral wall. Take 3 ml of venous blood without adding anticoagulant and coagulant, store it at room temperature for 1 hour until the blood is completely coagulated, centrifuge at 3000 rpm for 15 minutes, take serum, and centrifuge at 5000 rpm for 30 seconds to precipitate a small amount of suspended red blood cells. Obtain about 1ml of serum, take out 20ul for testing, and store the rest in a refrigerator at minus 80 degrees Celsius.

[0029] Protein chip preparation: Take 3 ul of patient serum, add 6 ul of 9mol / l urea, 2% CHAPS, 1% DTT buffer to dilute, mix well, ice bath for 30 minutes, then add 108 ul of 50mmol / l sodium acetate buffer to dilute. Take a weak cation exchange chip (WCX2), add 200ul 50mmol / l sodium acetate buffer solution to point A of the chip, place the protein chip on a shake...

Embodiment approach 2

[0033] Sample collection: 1 year after radiotherapy for nasopharyngeal carcinoma of the patient to be tested, a tumor in the left neck was found 2 months ago, CT showed changes in the side wall of the right nasopharynx, and 3 ml of venous blood was collected without adding anticoagulant or coagulant. Store at room temperature for 1 hour until the blood is completely coagulated, centrifuge at 3000 rpm for 15 minutes, take serum, and centrifuge at 4500 rpm for 30 seconds to precipitate a small amount of suspended red blood cells, obtain about 900ul of serum, take out 20ul for testing, and place the rest in liquid nitrogen for quick freezing Store in refrigerator at 20°C.

[0034] Protein chip preparation: Take 3 ul of patient serum, add 6 ul of 9mol / l urea, 2% CHAPS, 1% DTT buffer to dilute, mix well, ice bath for 30 minutes, then add 108 ul of 50mmol / l sodium acetate buffer to dilute. Take a weak cation exchange chip (WCX2), add 200ul 50mmol / l sodium acetate buffer solution to ...

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Abstract

The serum protein fingerprint pattern method for NPC detection comprises: preparing protein chip with a little serum to generate the fingerprint pattern, building the corresponding database with a great deal of serum samples and fingerprint patterns; detecting sufferer serum in biostatistics to compare by the database and decide the probability of NPC. This invention has sensitivity and specificity more than 80%.

Description

technical field [0001] The invention relates to the technical field of medical detection, and is a new non-invasive detection method for early detection and prognosis monitoring of nasopharyngeal carcinoma in vitro, and its sensitivity and specificity both reach more than 80%. Background technique [0002] The incidence and mortality of nasopharyngeal carcinoma are on the rise in my country, with the highest incidence in the Pearl River Delta and the Xijiang River Basin. The etiology of nasopharyngeal carcinoma is not yet known, and possible related factors include: Epstein-Barr virus infection, chemical carcinogenic factors, genetic factors, etc. At present, the clinical detection methods of the nasopharynx mainly include microscope examination, CT / MRI examination, B-ultrasound examination, EB virus antibody examination and tumor biopsy pathological examination, among which biopsy pathological examination is the only qualitative method, and EB virus antibody examination can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/48G01N30/86
Inventor 叶志海夏鲁全
Owner ZHUHAI BAIYUAN BIOLOGICAL INFORMATION TECH
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