Methods of immune or hematological enhancement, inhibiting tumour formation or growth, and treating or preventing cancer, cancer symptoms, or the symptoms of cancer treatments
A tumor and cancer technology, applied in the field of cancer symptoms or cancer treatment symptoms, treatment or prevention of cancer, can solve the problem that the response of tumor chemotherapy is not very good
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Embodiment 1
[0341] Bovine lactoferrin with greater than 90% purity is from Fonterra Co-operative Group. To prepare apo-Lf (apo-Lf), a solution of approximately 80 mg / mL Lf in milliQ water (pH~5.7) was adjusted to pH 2.08 by careful addition of 6M HCl. The solution was stirred at room temperature for 1 hour and then dialyzed against 10 volumes of 0.1 M citric acid overnight at 4°C using SpectraPor tubes with a nominal 3.5 kDa molecular weight cut-off (Spectrum Companies, Ranco Dominguez, CA, USA). The dialysate was changed twice within 24 h, and then the Lf solution was freeze-dried to a white semi-crystalline powder. To prepare 50% iron-saturated lactoferrin, a solution of 8% lactoferrin in 0.1M sodium bicarbonate was adjusted to pH 8.2 by careful addition of 6M NaOH. Addition of a suitable volume of 50 mM iron nitrilotriacetate (Fe-NTA) (Bates et al., 1967; Brock & Arzabe, 1976) yielded ~50% saturated lactoferrin (considering the purity of Lf and its natural ~12% iron saturation). Aft...
Embodiment 2
[0342] This example shows that milk fat inhibits the growth of EL-4 tumors, whereas milk fat enriched in conjugated linoleic acid and vacantoleic acid is ineffective.
[0343] Groups containing 6 C57BL / 6 mice were fed either basic AIN-93 chow, or the same chow replaced with 120 g of milk fat or fortified milk fat per 2.4 Kg of chow, representing -71% fat content of the chow. 2 x 10 5 EL-4 tumor cells were tested subcutaneously in mice. Tumor size (measured by perpendicular two diameters in centimeters) was monitored until day 91, or until tumors reached 1 cm in diameter. Each point represents the mean tumor size with 95% confidence interval for 6 mice or the indicated number of mice.
[0344] Compared with the control diet, fortified milk fat slowed tumor growth by 25% on day 49, but the effect was not significant ( figure 1 ). In sharp contrast, in 2 out of 6 mice milk fat completely tissue tumor development. Tumor growth in the other four mice fed the milk fat diet was ...
Embodiment 3
[0345] This example shows that milk fat works synergistically with immunotherapy to eradicate EL-4 tumors.
[0346] Tumors were established in groups of 5 mice fed fortified milk fat, milk fat or a control diet, as described in Example 2 above. Tumor size (measured by perpendicular two diameters in centimeters) was monitored. When tumors reached ~0.4 cm in diameter, DNA-liposome complexes containing 60 μg of B7-1 expression plasmid were injected into the tumors. The times of plasmid administration are indicated by arrows. Tumor size (measured by perpendicular two diameters in centimeters) was monitored until day 91, or until tumors reached 1 cm in diameter. Tumors of this particular size remained partially sensitive to B7-1 immune gene therapy, as evidenced by the fact that control tumors in four mice regressed slowly for a week and then grew again. However, during the 4 weeks after injection of the B7-1 plasmid, the tumor in 1 mouse regressed and disappeared ( figure 2 )...
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