Preparation method of human milk alternative fat combining enzymatic acidolysis and physical mixing

Abstract

The invention discloses a preparation method of human milk alternative fat combining enzymatic acidolysis and physical mixing. The preparation method mainly comprises the following two steps: S1, adjusting the composition (greater than 1%) of a plenty of fatty acids of catfish oil or a fractionation product of the catfish oil by virtue of acidolysis; and S2, adjusting the composition of trace fatty acids (less than 1%) of an enzymatic product by virtue of physical mixing. The raw materials catfish oil or the fractionation product of the catfish oil used by the method are wide in source and low in price, and contain a relatively high content of sn-2 palmitic acid and a relatively low content of total palmitic acid. In the acidolysis reaction, the free fatty acids used is small in proportion, and in the process, the composition and distribution of the plenty of fatty acids are emphasized at the same time as well as the composition of trace fatty acids containing polyunsaturated fatty acids with medium carbon chains and long carbon chains. The obtained product is highly similar to human milk fat and has a relatively huge market application prospect.

Description

technical field

[0001] The invention belongs to the technical field of oils and fats, and in particular relates to a preparation method of human milk substitute fat combined with enzymatic acid hydrolysis and physical mixing. Background technique

[0002] Fat accounts for 3-5% in human milk and is the main energy source (>50%) for newborns, of which triglycerides account for more than 98%. Human milk fat contains about 200 kinds of fatty acids, including medium carbon chain fatty acids, saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids, odd carbon chain fatty acids, branched chain fatty acids, trans fatty acids and hydroxy fatty acids. Among these fatty acids, those containing more than 1% are oleic acid (25-35%), palmitic acid (20-30%), linoleic acid (10-20%), stearic acid (5-9%), Myristic acid (4-9%), lauric acid (3-7%) and palmitoleic acid (1-3%). The fatty acid distribution of triglycerides in human milk fat is unique, more than 60% of ...

Examples

Embodiment 1

[0078] In a batch reactor, catfish oil was used as raw material, 1,3-position specific lipase Lipozyme RM IM was used as catalyst, the reaction temperature was 50 degrees, the amount of enzyme added was 10 wt%, and the stirring rate was 500 rpm. The ratio of free fatty acids derived from rapeseed oil rich in oleic acid and sunflower oil rich in linoleic acid is 1:3, the molar ratio of catfish oil to free fatty acids is 1:3, and the stirring speed is 600 rpm. After the reaction, the lipase in the reaction was removed by centrifugation, while free fatty acids were removed by distillation. The composition of the mixed fatty acids in different proportions used in acid hydrolysis is shown in Table 2, and the fatty acid composition and distribution of the obtained product are shown in Table 3.

[0079]Table 2. Rapeseed oil and sunflower oil fatty acid mixtures in different proportions

[0080] FA type

RSO

SFO

1:3

C12:0

0.90

1.30

1.20

C...

Embodiment 2

[0108] In a packed bed continuous reactor, the 30-degree solid fat fraction of catfish oil is used as raw material, and the 1,3-position-specific lipase Lipozyme RM IM is used as a catalyst. The reaction temperature is 60 degrees, and the residence time is 1h. The selected source The ratio of free fatty acids in tea seed oil rich in oleic acid and corn oil rich in linoleic acid is 1:2, and the molar ratio of catfish oil solid fat fraction to free fatty acids is 1:4, which is removed by centrifugation after reaction lipase in the reactant while removing free fatty acids by distillation. The composition and distribution of fat-solid fatty acids obtained by fractionating catfish oil at 30 degrees are shown in Table 6, the composition of mixed fatty acids is shown in Table 7, and the fatty acid composition and distribution of the resulting product are shown in Table 8.

[0109] Table 6 Fatty acid composition and distribution of catfish oil fractionated and solidified at 30 degrees...

technical field

[0001] The invention belongs to the technical field of oils and fats, and in particular relates to a preparation method of human milk substitute fat combined with enzymatic acid hydrolysis and physical mixing. Background technique

[0002] Fat accounts for 3-5% in human milk and is the main energy source (>50%) for newborns, of which triglycerides account for more than 98%. Human milk fat contains about 200 kinds of fatty acids, including medium carbon chain fatty acids, saturated fatty acids, monounsaturated fatty acids, polyunsaturated fatty acids, odd carbon chain fatty acids, branched chain fatty acids, trans fatty acids and hydroxy fatty acids. Among these fatty acids, those containing more than 1% are oleic acid (25-35%), palmitic acid (20-30%), linoleic acid (10-20%), stearic acid (5-9%), Myristic acid (4-9%), lauric acid (3-7%) and palmitoleic acid (1-3%). The fatty acid distribution of triglycerides in human milk fat is unique, more than 60% of ...

Examples

Embodiment 1

[0078] In a batch reactor, catfish oil was used as raw material, 1,3-position specific lipase Lipozyme RM IM was used as catalyst, the reaction temperature was 50 degrees, the amount of enzyme added was 10 wt%, and the stirring rate was 500 rpm. The ratio of free fatty acids derived from rapeseed oil rich in oleic acid and sunflower oil rich in linoleic acid is 1:3, the molar ratio of catfish oil to free fatty acids is 1:3, and the stirring speed is 600 rpm. After the reaction, the lipase in the reaction was removed by centrifugation, while free fatty acids were removed by distillation. The composition of the mixed fatty acids in different proportions used in acid hydrolysis is shown in Table 2, and the fatty acid composition and distribution of the obtained product are shown in Table 3.

[0079]Table 2. Rapeseed oil and sunflower oil fatty acid mixtures in different proportions

[0080] FA type

RSO

SFO

1:3

C12:0

0.90

1.30

1.20

C...

Embodiment 2

[0108] In a packed bed continuous reactor, the 30-degree solid fat fraction of catfish oil is used as raw material, and the 1,3-position-specific lipase Lipozyme RM IM is used as a catalyst. The reaction temperature is 60 degrees, and the residence time is 1h. The selected source The ratio of free fatty acids in tea seed oil rich in oleic acid and corn oil rich in linoleic acid is 1:2, and the molar ratio of catfish oil solid fat fraction to free fatty acids is 1:4, which is removed by centrifugation after reaction lipase in the reactant while removing free fatty acids by distillation. The composition and distribution of fat-solid fatty acids obtained by fractionating catfish oil at 30 degrees are shown in Table 6, the composition of mixed fatty acids is shown in Table 7, and the fatty acid composition and distribution of the resulting product are shown in Table 8.

[0109] Table 6 Fatty acid composition and distribution of catfish oil fractionated and solidified at 30 degrees...

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