54 results about "Granulocytopenias" patented technology

The invention disclosed herein relates to compositions and methods for treating subjects suffering from or at risk of developing neutropenia. In some embodiments, the methods comprise administering to a subject suffering from or at risk of developing neutropenia, an effective amount of pirfenidone and one or more toll-like receptor (TLR) agonists.

Neutropenia is the dose-limiting toxicity of the tri-weekly docetaxel (Taxotere®) schedule. Here, we evaluate in Metastatic Breast Cancer (MBC) patients (N=38) a computerized method for predicting docetaxel-induced neutropenia, and use the model to identify improved docetaxel and Granulocyte Colony Stimulating Factor (G-CSF) regimens. Pharmacokinetics / pharmacodynamics (PK / PD) models were created and simulated concomitantly with a mathematical granulopoiesis model. Individual baseline neutrophil counts and docetaxel schedules served as inputs. Our trial validated the model accuracy in predicting nadir timings (r=0.99), grade 3 / 4 neutropenia (86% success) and neutrophil profiles (r=0.62). Model was robust to CYP3A-induced variability, except for slightly less accurate grade 3 / 4 neutropenia predictions. Simulations confirm smaller toxicity of the weekly docetaxel regimen than the tri-weekly one, and suggest an optimal G-CSF support for alleviating neutropenia, 60 μg / day QD×3, 6-7 days post-docetaxel, administered tri- and bi-weekly, and 4 days post weekly docetaxel>33 mg / m2.

The invention discloses a pharmaceutical preparation containing recombinant human serum albumin-human granulocyte colony stimulating factor fusion protein and preparation of the pharmaceutical preparation. According to the invention, the medical preparation containing the rHSA / G-CSF (recombinant human serum albumin-human granulocyte colony stimulating factor fusion protein) is a freeze-drying preparation, and each freeze-drying preparation comprises the following components: 1-30mg of rHSA / G-CSF protein, 10-80mg of pharmaceutically acceptable water-soluble excipient, 5-30mg of pharmaceutically acceptable protective agent and 5-50 mu mol of pH regulator. The pharmaceutical preparation containing the rHSA / G-CSF protein is suitable for administration in the ways of subcutaneous injection or intravenous injection and the like after being dissolved in water for injection, and the pharmaceutical preparation can be injected for the right dosage for treatment of neutrophilic granulocytopenia.

The invention relates to the use of compounds to ameliorate or treat an condition such as a cystic fibrosis, neutropenia or other exemplified conditions. Exemplary compounds that can be used include 3β-hydroxy-17β-aminoandrost-5-ene, 3β-hydroxy-16α-fluoro-17β-aminoandrost-5-ene, 3α-hydroxy-16α-fluoro-17β-aminoandrost-5-ene, 3β-hydroxy-16β-fluoro-17β-aminoandrost-5-ene, 1α,3β-dihydroxy-4α-fluoroandrost-5-ene-17-one, 1α,3β,17β-trihydroxy-4α-fluoroandrost-5-ene, 1β,3β-dihydroxy-6α-bromoandrost-5-ene, 1α-fluoro-3β,12α-dihydroxyandrost-5-ene-17-one, 1α-fluoro-3β,4α-dihydroxyandrost-5-ene and 4α-fluoro-3β,6α,17β-trihydroxyandrostane.

Means for determining the presence of the risk of drug-induced granulocytopenia in a human is provided. A method for assessing the risk of drug-induced granulocytopenia, including detecting a polymorphism of the human insulin receptor substrate-2 gene of a subject, and determining the presence of the risk of drug-induced granulocytopenia of the subject by use of the genetic polymorphism as an index.

A method of treatment for feline leukemia virus infections by continuously administering a feline interferon preparation containing a feline interferon as a main component daily to a cat is disclosed. As a feline interferon, a feline omega (omega)-interferon is preferably used, and more particularly, a recombinant interferon is preferably used. A method of treatment using a therapeutic agent containing a feline omega-interferon as a main component in accordance with the present invention is a novel and superior method suitable for treating feline leukemia virus infections, and in particular, for treating neutropenia.

Disclosed herein are plinabulin and the use for reducing neutropenia. Some embodiments relate to reducing the docetaxel induced neutropenia using plinabulin.

The invention relates to a G-CSF (Granulocyte-Colony Stimulating Factor) fusion protein mutant, a preparation method and application thereof. The G-CSF fusion protein mutant is fusion protein having the effect of stimulating the proliferative activity of neutrophile granulocytes, and the structure of the G-CSF fusion protein mutant is G-CSF / carrier protein or carrier protein / G-CSF, wherein the G-CSF part contains multi-site mutations, which changes the activity and the receptor affinity. Compared with the traditional like products, the G-CSF fusion protein mutant has longer half-life period and higher biologic activity. Through injecting a proper dosage of the medicinal preparation, the neutrophilic granulocytopenia can be treated.

The present invention relates to novel compounds and pharmaceutical compositions thereof, and methods for inhibiting the activity of SHP2 phosphatase with the compounds and compositions of the invention. The present invention further relates to, but is not limited to, methods for suppressing tumor cell growth, ameliorating the pathogenesis of systemic lupus erythematosus, and the treatment of various other disorders, including Noonan syndrome, diabetes, neutropenia, neuroblastoma, melanoma, juvenile leukemia, juvenile myelomonocytic leukemia, chronic myelomonocytic leukemia, acute myeloid leukemia, and other cancers associated with SHP2 deregulation with the compounds and compositions of the invention, alone or in combination with other treatments. Other cancers associated with SHP2 deregulation include HER2-positive breast cancer, triple-negative breast cancer, ductal carcinoma of the breast, invasive ductal carcinoma of the breast, non-small cell lung cancer, esophageal cancer, gastric cancer, squamous-cell carcinoma of the head and neck (SCCHN), and colon cancer.

A compound for use in a method of treatment of cancer, including precancerous lesions, and adverse events caused by the disease or anti-cancer agents and treatments, such as cancer cachexia, lymphopenia, neutropenia, febrile neutropenia includes in combination an immunomodulatory and at least one anti-cancer agent or treatment suitable for treating the disease. The immunomodulator is a proteic aggregate of ammonium and magnesium phospholinoleate-palmitoleate anhydride. The anti-cancer agent or treatment suitable for treating the disease provides synergistic effects without additional toxicity when used with the immunomodulatory. The anti-cancer treatment is selected from the following group: surgical procedures, transplantation of bone marrow cells, systemic and localized radiotherapy, and combinations thereof.

The invention relates to the use of compounds to ameliorate or treat an condition such as a cystic fibrosis, neutropenia or other exemplified conditions. Exemplary compounds that can be used include 3β-hydroxy-17β-aminoandrost-5-ene, 3β-hydroxy-16α-fluoro-17β-aminoandrost-5-ene, 3α-hydroxy-16α-fluoro-17β-aminoandrost-5-ene, 3β-hydroxy-16β-fluoro-17β-aminoandrost-5-ene, 1α,3β-dihydroxy-4α-fluoroandrost-5-ene-17-one, 1α,3β,17β-trihydroxy-4α-fluoroandrost-5-ene, 1β,3β-dihydroxy-6α-bromoandrost-5-ene, 1α-fluoro-3β,12α-dihydroxyandrost-5-ene-17-one, 1α-fluoro-3β,4α-dihydroxyandrost-5-ene and 4α-fluoro-3β,6α,17β-trihydroxyandrostane.

This invention relates to a use of G-CSF dimer in the treatment of neutropenia. In particular, the recombinant human G-CSF of the present invention can enhance the differentiation and development of neutrophils in animal, and thus effectively reduce the severity of the severe neutropenia and shorten the time of severe neutropenia for the post-chemotherapy cancer patients. Serum half-life of G-CSF dimer of this invention is prolonged and the biological activity thereof is increased, providing a better effect in the treatment of neutropenia.

Disclosed herein are methods of reducing neutropenia that is caused by chemotherapy or radiation therapy by administering plinabulin to a subject.

The invention discloses a primer and a method for detecting mutation of an ELA2 gene of a patient with congenital neutropenia. The primer comprises a primer which is used for amplifying the whole exon sequence of the ELA2 gene; the Sanger sequencing technology and a sequencing primer are adopted. According to the method and the primer, the mutation of the whole exon sequence of the ELA2 gene in the body of the patient with the congenital neutropenia can be detected rapidly. The detection result finished by the method and the primer is accurate, can assist in diagnosing the congenital neutropenia, and has an important reference meaning for early intervention, early treatment and antenatal diagnosis.

The present invention concerns compounds modulating apoptosis of neutrophil cells. In particular, the invention concerns compounds inhibiting an interaction between Proliferating Cell Nuclear Antigen (PCNA) and proteins binding to cytoplasmic PCNA in neutrophil cells, for use in the treatment of a disease involving a neutrophil-dependent inflammatory process. The invention also relates to a method for the identification of a compound for use in the treatment of a neutrophil-dependent inflammatory process. The invention further relates to peptides for use in the treatment of neutropenia.

The invention discloses a method for the fixed-point coupling of polyethylene glycol on a nitrogen-terminal of a colony stimulating factor of a column chromatography granulocyte, wherein the coupling method comprises the following steps that: 1) a chromatography medium is preferred to be an exchange medium; 2) the height of a column bed of a chromatography column is preferred to be the upper limit of the recommended height of the column; 3) the sample volume of rhG-CSF protein is preferred to be 30 to 50 percent of the actually measured maximum loading capacity of the medium; 4) the activated mPEG is sampled under conditions of lower sample flow rate, longer sample time (3 to 5 hours) and higher pH value of reaction rate; 5) the sample volume of the activated mPEG is made according to the reaction volume ratio of the activated mPEG to the rhG-CSF of 1-50mol:1mol; and 6) 0.5 to 1M of salt solution of sodium chloride is used for gradient elution. The invention also discloses a coupling compound prepared by the method, which is a pegylation recombination human granulocyte colony stimulating factor, of which the structural formula is CH3-(CH2CH2-O)n-(CH2)r-NH-rhG-CSF or CH3-(CH2-CH2-O)n-(CH2)r-NH-rhG-CSFm, wherein n is between 570 and 2,200, and r is between 1 and 3; moreover, the invention also discloses application of the coupling compound in the preparation of a preparation preventing and treating the granulocytopenia.

The invention relates to a method for treating or preventing neutropenia in a patient receiving chemotherapy by administering a multi-PEGylated granulocyte colony stimulating factor (G-CSF) variant on the same day that chemotherapy is administered.

An acid-modified arabinogalactan protein composition, having an arabinose-galactose ratio of less than 3.5:1 or less than 80% of the arabinose:galactose ratio of the arabinogalactan protein component of the composition prior to acid modification, prepared from Astragalus membranaceus, especially from the roots of Atragalus membranaceus, is capable of reconstitution into an aqueous intravenously injectable formulation; and is useful for stimulating hematopoiesis, inducing the proliferation or maturation of megakaryocytes, stimulating the production of IL-1beta, Il-6, TNF-alpha, IFN-gamma, GM-CSF, or G-CSF, stimulating the production or action of neutrophils, treating neutropenia, anemia, or thrombocytopenia, accelerating recovery from exposure (e.g. accidental or non-therapeutic exposure, as well as therapeutic exposure) to cytotoxic agents or radiation, treating cachexia, emesis, or drug withdrawal symptoms, or modifying biological responses or protecting hepatic cells in hepatitis B, in a mammal when intravenously administered to the mammal.

The present invention relates to a novel peptide exhibiting an effect of releasing myelopoiesis stem cells into blood and an osteoporosis therapeutic effect and use thereof and, in particularly, to a novel peptide consisting of an amino acid sequence of SEQ. ID.NO:1 which has effects of releasing hematopoietic stem cells into a bloodstream and decreasing osteoclast cells in bone narrow, and a pharmaceutical composition comprising the novel peptide as an active ingredient for preventing or treating neutropenia, anemia or osteoporosis. Because of side effects, the peptide of the present invention not only increases level of leukocytes, red blood cells and platelets in blood, but also alleviates a decrease in bone density, and therefore, can be very usefully used for the development of a prophylactic or therapeutic agent for neutropenia, anemia or osteoporosis.

The present invention relates to pharmaceuticals and medicine, namely, to new physiologically active conjugates of granulocyte colony-stimulating factor (G-CSF) with the general formula (I) where: n - integers from 681 to 1 000; m - integer > 4; NaH-G-CSF - natural or recombinant polypetide, having the activity of G-CSF. The invention is also related to medicines containing the claimed conjugate of formula (I), pharmaceutical compositions, the use of conjugate of formula (I) for drugs and medicines with granulocyte colony stimulating factor as an active ingredient, approaches to prevent and / or treat neutropenia, the container that comprises pharmaceutical composition.

The invention is directed to a method of treating severe neutropenia, and in particular, cyclic neutropenia (CN) or severe congenital neutropenia (SCN), in a patient in need of such treatment comprising: administering a therapeutically effective amount of a compound of Formula (I) or a pharmaceutically acceptable salt thereof.

The present invention relates to a mutant G-CSF fusion protein. The mutant G-CSF fusion protein is a fusion protein having the activity of stimulating the proliferation of neutrophilic granulocytes, and having a basic structure of G-CSF / carrier protein or carrier protein / G-CSF; wherein the G-CSF moiety comprises multipoint substitutions thus resulting in changes in biological activity and binding affinity. Compared with existing products, the mutant G-CSF fusion protein in the present invention has longer half-life and higher biological activity. Administration of the pharmaceutical preparation containing this mutant G-CSF fusion protein could be used in the treating neutropenia.

The invention is directed to a method of treating severe neutropenia, and in particular, cyclic neutropenia (CN) or severe congenital neutropenia (SCN), in a patient in need of such treatment comprising: administering a therapeutically effective amount of a compound of Formula (I) or a pharmaceutically acceptable salt thereof.

The invention discloses a medicinal composition which is prepared from he following Chinese medicinal herbs (by weight ratio), notoginseng 10-30%, ginseng 10-10%, ophiopogon root 40-80%. The invention also discloses the process for preparing the pharmaceutical composition and use thereof.

It is intended to disclose a method for risk estimation of the onset of granulocytopenia in a subject caused by paclitaxel treatment. This method comprises identifying gene polymorphisms in genes isolated from the subject, i.e., at five SNPs (IMS-JST111898 (SEQ ID NO:1), IMS-JST105874 (SEQ ID NO:2), IMS-JST082397 (SEQ ID NO:3), IMS-JST071852 (SEQ ID NO:4) and IMS-JST071853 (SEQ ID NO:5)) in CYP2C8 gene, and at five SNPs (IMS-JST074538 (SEQ ID NO:6), IMS-JST079837 (SEQ ID NO:7), IMS-JST044164 (SEQ ID NO:8), IMS-JST063023 (SEQ ID NO:9) and IMS-JST042569 (SEQ ID NO:10)) in BUB1b gene. Also, a kit containing a reagent to be used in this method is disclosed.

A method for preventing neutropenia or reducing the duration of neutropenia in a patient by administering therapeutically effective amount of a human hybrid (hy) Fc fused granulocyte colony stimulating factor (G-CSF) developed as next-generation G-CSF.

The invention is based, in part, on the discovery of a novel cell-based immunotherapy that can recapitulate neutrophil functions in neutropenic individuals afflicted with a microbial infection. The therapeutic methods of the invention are broadly applicable to treat any infection in a neutropenic individual, including infections caused by bacteria, fungi, protozoa, and viruses. The methods of the invention represent a practical, rapid cell-based immunotherapy for refractory infections comprising compositions of activated, irradiated HL-60 cells.