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40 results about "Hl 60 cell" patented technology
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Biosensor for detecting peptidylarginine deiminases, and preparation method and application thereof
InactiveCN107271511AEasy to distinguishIncreased sensitivityMaterial analysis by electric/magnetic meansHl 60 cellCucurbituril
The invention relates to a biosensor for detecting peptidylarginine deiminases, and a preparation method and application thereof. The sensor is characterized in that a polypeptide chain 1 (FGGGRGAC) is modified on the surface of a gold electrode; the sulfydryl of the cysteine at the end C of the polypeptide chain fixes the polypeptide chain 1 onto the surface of the electrode through a gold-sulfur bond; polypeptide chain 2 (FGGGGGC) functional silver nanometer particles are connected with silver nanometer particles through the sulfydryl of cysteine of the polypeptide chain 2. Under the assistance of the mutual action of supermolecule cucurbituril 8(CB[8]) assisted subject and object, the functional silver nanometer particles can be recognized with FGG at the end N of the polypeptide chain 1 and FGG at the end N of the polypeptide chain 2 due to CB[B]. The biosensor uses a super molecular chemistry assistance signal marking method for improving the experiment sensitivity; the linear range of the PAD4 can be detected to be 0.005 nM to 200 nM; the detection limit is 0.79 nM; the specificity is very high; the target and other reference protein can be effectively distinguished; the goal of detecting the activity of the endogenous PAD4 in the HL-60 lysates can be achieved.
Owner:SHANGHAI UNIV
Alpha, beta-unsaturated ketone compound containing 1,2,4-oxadiazoles heterocycle
ActiveCN101891697AEnhanced growth inhibitory activityStrong growth inhibitory effectOrganic active ingredientsOrganic chemistryAntileukemic agentHydrogen
The invention relates to an alpha, beta-unsaturated ketone compound containing a 1,2,4-oxadiazoles heterocycle, the structure of which is as shown in general formula 5, wherein R1 is hydrogen, methyl or chlorine; R2 is methyl, chlorine or bromine; R3 is methyl or ethyl; R4 is para-trifluoromethyl, ortho-methyl or para-nitro; and R5 is hydrogen or methyl. The invention also provides a preparation method of the compound and application thereof. The half-growth inhibitory concentration of the compound on HL-60 cells is less than or equal to 2.0 mu M, and the activity is obviously improved, thus the compound can be used for preparing antileukemie medicaments.
Owner:山东康瑞健医疗技术有限公司
Polyoxometalate compound modified by amino acid and preparation method and application of polyoxometalate compound
ActiveCN105732409ASolve the problem of low anti-leukemia activityHigh activityOrganic chemistryAntineoplastic agentsHl 60 cellArginine
The invention discloses a polyoxometalate compound modified by amino acid.The compound has the general formula of Am[B3XMo6O21].nH2O and is prepared through the steps of dissolving Na2MoO4.H2O, Se, As, Sb or Bi oxide, glycine, alanine, histidine, lysine, tryptophan, phenylalanine, methionine, threonine, isoleucine, leucine or arginine and KCl in H2O, putting the solution on a magnetic stirrer to be mixed and stirred, adjusting pH of the solution to be 3.4 to 3.6 through HCl, stirring and heating reflux for 0.5-2 hours, conducting cooling and filtering, standing for 1-2 days, and collecting crystals.It is determined through the MTT method that the IC50 value of HL-60 cells is 0.127 microM, the IC50 value of U937 cells is 2.731 microM, the IC50 value of U937 cells is 2.731 microM, and the IC50 value of HUVECs cells is 889.18 microM.The polyoxometalate compound is high in leukemia resistant activity, low in toxicity and low in price.
Owner:JILIN UNIV
Application of snake venom-sourced cytotoxin-CTX1 to preparation of acute leukemia resisting medicine
InactiveCN102526704ASequence clearSingle ingredientPeptide/protein ingredientsAntineoplastic agentsHl 60 cellSnake venom
The invention discloses application of a snake venom-sourced cytotoxin-CTX1 to preparation of an acute leukemia resisting medicine. Experiments prove that the snake venom-sourced cytotoxin-CTX1 can inhibit the proliferation of a leukemia cell, can induce non-viable apoptosis and necrosis of HL-60 cell and KG1a cell, and has obvious medicinal effect in the aspect of leukemia resistance; therefore, the snake venom-sourced cytotoxin-CTX1 can be further developed into anti-tumor medicines with high efficiency, low toxicity and low drug tolerance.
Owner:广州医学院
Use of water chestnut extract in preparing medicament for treating leukaemia
InactiveCN101433564ASimple processEasy to operateAntineoplastic agentsPlant ingredientsWater ChestnutsHl 60 cell
The invention relates to application of water chestnut extract to preparing medicament for treating leukemia, and belongs to the field of medicine. The application of the water chestnut extract to preparing the medicament for treating the leukemia is application of the water chestnut extract to preparing the medicament for inhibiting HL-60 cell proliferation. The water chestnut extract treats the leukemia through inducing HL-60 cell apoptosis. The preparation method for the water chestnut extract has the advantages of simple process, convenient operation, easily grasped technique, and low production cost.
Owner:JILIN UNIV
Medicine novel use of procyanidine oligomer and multimer
The invention provides novel application of proanthocyanidin oligomer F2 and a polymer F3 in preparing anti-tumor health care food and medicine, in particular novel application in preparing health care food and medicine for treating glioma. The application researches inhibiting function of F2 on brain tumor, blood system tumor, alimentary canal tumor, respiratory system tumor and urinary genital system tumor. Firstly, F2 and F3 are discovered to have the inhibiting functions on glioma cell strains U-87 and U251, humanized leukemia HL-60 cell strains, humanized liver cancer Hep3B cell strains, humanized colon cancer Colon-205 cell strains, humanized prostate cancer Du-145 cell strains and humanized A549 lung cancer cell strains, and the inhibiting function is improved along with progressive increase of dosage. In addition, the F2 can also remarkably inhibit chemotaxis of human malignant glioma U-87 cells induced by formyl peptide and expression of formyl peptide receptor (FPR) in the cells. The receptor has important function on mediating migration, survival, growth and vasculogenesis of the human malignant glioma cells.
Owner:SHENYANG PHARMA UNIVERSITY
Alpha, beta-unsaturated ketone compound containing thiazole heterocycle, its preparation method and application
ActiveCN102516196AGood growth inhibitory activityInhibitory activityOrganic chemistryAntineoplastic agentsHydrogenThiazole
The invention relates to an alpha, beta-unsaturated ketone compound containing thiazole heterocycle, its preparation method and application. The alpha, beta-unsaturated ketone compound containing thiazole heterocycle is shown by the general formula 8, wherein R1 represents hydrogen, methyl or chlorine, R2 represents methyl or chlorine, R3 represents methyl or ethyl, R4 represents hydrogen, phenyl, p-nitro phenyl, p-benzotrifluoride or naphthyl, R5 represents hydrogen, phenyl, p-nitro phenyl, p-benzotrifluoride or naphthyl. The invention also provides the preparation method and application of the compound. The compound has excellent activity for inhibiting GST Pi and activity for inhibiting HL-60 cell growth, and is used for preparing antitumor drugs.
Owner:SHANDONG UNIV
Purification process of bupleurun chinense polysaccharide fragment and application thereof in preparation of anti-inflammatory drug
ActiveCN105713103AConfirmed anti-inflammatory activityOrganic active ingredientsAntipyreticP-selectinPeritoneal fluid
The invention belongs to the field of natural medicinal chemistry and particularly relates to a separation and purification process of natural polysaccharide type anti-inflammatory drug molecules and application thereof. The separation and purification process utilizes water extraction and alcohol sedimentation, protein removal, acid hydrolysis, freeze drying, desalination, affinity chromatography and other methods to purify a polysaccharide fragment having a P-selectin antagonism function from bupleurun chinense. Flow cytometry and protein interaction analysis experiment results show that the bupleurun chinense polysaccharide fragment can effectively prevent the combination between P-selectin and HL-60 cells and the antagonism to the mutual effect of the P-selectin and PSGL-1. The experimental results of a parallel-plate flow chamber and an acute abdominal inflammation mouse model show that the bupleurun chinense polysaccharide fragment has the remarkable inhibitory effect on the HL-60 cells mediated by the P-selectin in a shear stress state and can effectively inhibit leukocytic exudation from the peritoneal fluid of a mouse. The bupleurun chinense polysaccharide fragment prepared by means of the method has good anti-inflammatory activity and has a broad application prospect by serving as a novel anti-inflammatory drug.
Owner:WENZHOU UNIVERSITY +1
Screening method and application of aptamer for detecting acute promyelocyte leukemia
The invention provides an aptamer specifically combining acute promyelocyte leukemia HL-60 cells, and further provides a screening method and a clinical application of the aptamer. According to the screening method, six types of aptamers with different sequences are screened, and combining capacity among the aptamers and the acute promyelocyte leukemia HL-60 cells is different. The screening method includes the steps: synthesizing random single-strand DNA (deoxyribonucleic acid) libraries and primers; performing Cell-SELEX screening; performing library amplification; preparing a DNA single-strand library; repeating multiple screening and subduction screening; detecting stability after screening; detecting specificity of the aptamers.
Owner:BEIHUA UNIV
2-arylimidazole-phenanthroline probe capable of recognizing cu<2+> in HL-60 and preparation method thereof
InactiveCN107513063AHigh sensitivityHigh responseOrganic chemistryFluorescence/phosphorescenceHl 60 cellFluorescence
The invention discloses a 2-arylimidazole-phenanthroline probe capable of recognizing Cu<2+> in HL-60 and a preparation method thereof. The invention provides 2-arylimidazole[5,6-f] phenanthroline is applied to detection on Cu<2+> in HL-60 cells, has high sensitivity and high response value to Cu<2+>, and has obvious response to Cu<2+> when being used as a fluorescent probe. When being used for detecting Cu<2+> of different concentrations, the probe shows a good linear relationship, and has a good selectivity for Cu<2+> in HL-60 cells; after the HL-60 suspension cells stained with the compound 2-arylimidazole[5,6-F] phenanthroline are subjected to blue light excitation, the cells emit green fluorescence under the observation in a microscope, and the detection method is reliable; the reaction formula is as follows (shown in the description).
Owner:FUJIAN MEDICAL UNIV
Application of LukS-PV protein in preparation of medicine for inducing leukemia cell differentiation
InactiveCN103877563ASmall molecular weightEase of mass productionPeptide/protein ingredientsAntineoplastic agentsAberrant spleenHl 60 cell
The invention discloses an application of LukS-PV protein in preparation of a medicine for inducing leukemia cell differentiation. The leukemia cell refers to a human promyelocyte leukemia cell HL-60 or human monocyte leukemia cell THP-1. The LukS-PV protein can be massively expressed by using a genetic engineering technology, can be specifically combined with a target cell but not cause the dissolution of the target cell, is small in molecular weight, is easily modified genetically and chemically; and through gene modification, the induction activity is expected to be improved and the toxic and side effects are expected to be reduced; experiment shows that LukS-PV can induce the HL-60 cell to differentiate to marrow monocyte in vitro and induce the THP-1 cell to differentiate to macrophage; LukS-PV can inhibit leukemia cell growth in vivo and reduce infiltration of the leukemia cell to the liver, the spleen and the peripheral blood; therefore, the LukS-PV protein is expected to be developed into a novel leukemia treatment medicine, thereby opening up a novel application field for bacterial toxin.
Owner:ANHUI PROVINCIAL HOSPITAL
Application of ainsliaidimer A in preparing nucleolus transcription factor inhibitor medicament
InactiveCN102319239AHigh clinical application valueOrganic active ingredientsSenses disorderDiseaseHl 60 cell
The invention provides an application of a compound ainsliaidimer A in preparing a nucleolus transcription factor inhibitor medicament. The inventor carries out experiments of detecting activity of NF (nuclear factor)-kappa B by adopting EMSA (electrophoretic mobility shift assay), detecting differences of NF-kappa B in cells by immunochemical luminescent assay, detecting expression of the NF-kappa in ainsliaidimer A acting HL-60 cells by a Western blot method, detecting expression of apoptosis related protein in lymphoma cell CA46 cell after action of the ainsliaidimer A by the Western blot method, detecting influence of the ainsliaidimer A on HL-60 cell secretion VEGF (vascular endothelial growth factor) and the like. Results prove that the ainsliaidimer A has a good inhibition effect to the activity of the NF-kappa B abnormally activated by tumor cells, so that, the compound ainsliaidimer A serving as an active component can be used for preparing a nucleolus transcription factor inhibitor medicament. The medicament can be used as novel application for treating leukemia, autoimmune diseases, renal diseases, heart cerebrovascular diseases, various inflammations, neurodegenerative disorders and eye disorders of animals and humans caused by abnormally activation of the NF-kappa B factor, thereby providing a new method for treating the diseases and disorders, and having higher clinical application value.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Cell line method pyrogen detection method and kit
ActiveCN104762362AHigh sensitivityWide detection rangeMicrobiological testing/measurementTumor/cancer cellsHl 60 cellCultured cell
The invention discloses a cell line method pyrogen detection method and a kit. The method includes following steps: (1) a pyrogen material standard solution and a to-be-detected sample solution is prepared; (2) the pyrogen material standard solution or the to-be-detected sample solution are respectively added into a HL-60 cell-containing cell culture solution for culture, and cultured cell supernatant is taken; and (3) the content of cytokine IL-6 in the cultured cell supernatant of the step (2). According to the method, human body pyrogen reaction can be simulated, Gram-negative bacterium sourced, Gram-positive bacterium sourced and yeast sourced pyrogen materials can be qualitatively and quantitatively analyzed, sensitivity is high, and the method is simple and easy. The kit includes the pyrogen material standard solution, a cytokine IL-6 detection agent and HL-60 cells, the kit has the advantages of simple operation, capability of pyrogen material qualitative and quantitative analysis, and high sensitivity.
Owner:SHANGHAI INST FOR FOOD & DRUG CONTROL
Nano preparation for improving stability of enzyme drugs and preparation method and application of nano preparation
The invention belongs to the technical field of biopharmacy, and relates to a nano preparation method for improving stability of enzyme drugs. The nano preparation is nanoparticles PEG-DGL / En-Aco prepared from cis-acotonic anhydride (Aco), dendritic poly-l-lysine (DGL) and functional polyethylene glycol (NHS-PEG). The entrapment efficiency of the prepared nanoparticles is 71.46+ / - 0.31%, the appearance of the nanoparticles is round, and the nanoparticles are spherical; by the nanoparticles, degradation of trypsin to CAT can be relieved, in 72 h, the enzymatic activity of PEG-DGL / CAT-Aco nanoparticles in HL-60 cells is remarkably higher than free CAT, and in an extracellular matrix, the enzymatic activity of crosslinked nanoparticles is gradually increased along with time; by the prepared nanoparticles, the area of cerebral infarction of an animal pattern can be remarkably reduced; and ischemia reperfusion injury can be inhibited obviously, and the nano preparation has potential nerve and blood vessel protection effect. The prepared nanoparticles can be used for preparing drugs for treating stroke.
Owner:FUDAN UNIV
Detection method for regulating Hh signal channel to inhibit HL-60 cells
The invention discloses a detection method for regulating an Hh signal channel to inhibit HL-60 cells. The detection method comprises the steps as follows: proliferation of HL-60 cells among different experiment groups is detected with a kit, the HL-60 cell apoptosis rate of each group is detected through double staining, expression of Hedgehog signal channel components, namely, GLI1 genes and apoptosis genes BCL-2 and BCL-XL, of each experiment group is detected with a semi-quantitative RT-PCR method, and expression of GLI1 protein is detected with an immunofluorescence method. A co-culture mode is established in vitro by use of human acute myelocytic leukemia cell strains HL-60 and HS-5 cells, the interaction of in-vivo leukemia cells and hemopoietic microenvironment around the leukemia cells is simulated, the possible mechanism of the effect and action of the hemopoietic microenvironment on leukemia cell apoptosis is researched, and a theoretical foundation is laid for studying leukemia treatment drugs targeted at blocking the interaction of the hemopoietic microenvironment and the leukemia cells.
Owner:SHIHEZI UNIVERSITY
Detection method for effects of inhibitor GANT61 on multiplication and apoptosis of HL-60
The invention discloses a detection method for effects of an inhibitor GANT61 on multiplication and apoptosis of HL-60. The detection method comprises the following steps: detecting the growth and multiplication conditions of HL-60 cells with inhibitors of different concentrations through a CCK-8 (Cell Counting Kit-8) method, detecting the apoptosis condition of the HL-60 cells with an inhibitor through double staining, detecting the expression condition of the HL-60 cells with inhibitors of different concentrations through RT-PCR, and detecting the expression condition of proteins of the HL-60 cells with inhibitors of different concentrations through an immunofluorescence technology. According to the detection method, expression of a Hedgehog-GLI passageway is inhibited by a Hedgehog-GLI signal passageway specific inhibitor taking GLI as a target, and when the Hedgehog-GLI passageway is inhibited, the multiplication and apoptosis conditions of acute myeloid leukemia cells are observed; and a new treatment strategy is supplied to treatment of leucocythemia.
Owner:SHIHEZI UNIVERSITY
Heteropolyvanadate compound and preparation method thereof
The invention discloses a heteropolyvanadate compound which has a molecular formula [NH2(CH3)2]12[(V5O9Cl)6(TATB)8]. A preparation method comprises the following steps: dissolving VCl4 and 4,4,4-triazine-2,4,6-tribenzoic acid into 2ml of N,N-dimethyl formamide and 0.5mL of an acetonitrile solution, and carrying out stirring at room temperature; carrying out crystallization for 40-60 hours at 125-135 DEG C; reducing the temperature, separating a green crystal, carrying out washing, and carrying out room-temperature drying, so as to obtain the heteropolyvanadate compound. In-vitro experiment results show that median inhibitory concentrations of the salt upon cells SMMC-7721, MCF-7, A549 and HL-60 are respectively 0.67[mu] mol / L, 0.44[mu]mol / L, 0.48[mu]mol / L and 2.09[mu]mol / L; and in-vivo experiment results show that the salt is capable of reducing weights of tumors of Hep-A-22 liver cancer tumor-bearing mice.
Owner:JILIN UNIV
Use of water chestnut extract in preparing medicament for treating leukaemia
InactiveCN101433564BSimple processEasy to operateAntineoplastic agentsPlant ingredientsWater ChestnutsHl 60 cell
The invention relates to application of water chestnut extract to preparing medicament for treating leukemia, and belongs to the field of medicine. The application of the water chestnut extract to preparing the medicament for treating the leukemia is application of the water chestnut extract to preparing the medicament for inhibiting HL-60 cell proliferation. The water chestnut extract treats theleukemia through inducing HL-60 cell apoptosis. The preparation method for the water chestnut extract has the advantages of simple process, convenient operation, easily grasped technique, and low production cost.
Owner:JILIN UNIV
Methods for treating refractory infections in neutropenic individuals
The invention is based, in part, on the discovery of a novel cell-based immunotherapy that can recapitulate neutrophil functions in neutropenic individuals afflicted with a microbial infection. The therapeutic methods of the invention are broadly applicable to treat any infection in a neutropenic individual, including infections caused by bacteria, fungi, protozoa, and viruses. The methods of the invention represent a practical, rapid cell-based immunotherapy for refractory infections comprising compositions of activated, irradiated HL-60 cells.
Owner:LOS ANGELES BIOMEDICAL RES INST AT HARBOR UCLA MEDICAL CENT
A method for detecting pyrogens by cell line method and its kit
ActiveCN104762362BRandom combinationCommercially availableMicrobiological testing/measurementTumor/cancer cellsHl 60 cellGram-positive bacterium
The invention discloses a cell line method pyrogen detection method and a kit. The method includes following steps: (1) a pyrogen material standard solution and a to-be-detected sample solution is prepared; (2) the pyrogen material standard solution or the to-be-detected sample solution are respectively added into a HL-60 cell-containing cell culture solution for culture, and cultured cell supernatant is taken; and (3) the content of cytokine IL-6 in the cultured cell supernatant of the step (2). According to the method, human body pyrogen reaction can be simulated, Gram-negative bacterium sourced, Gram-positive bacterium sourced and yeast sourced pyrogen materials can be qualitatively and quantitatively analyzed, sensitivity is high, and the method is simple and easy. The kit includes the pyrogen material standard solution, a cytokine IL-6 detection agent and HL-60 cells, the kit has the advantages of simple operation, capability of pyrogen material qualitative and quantitative analysis, and high sensitivity.
Owner:SHANGHAI INST FOR FOOD & DRUG CONTROL
Application of 280 nm LED UV in malignant tumor autotransplantation purging in vitro under low oxygen condition
PendingCN110172403AResidue reductionLow recurrence rateBioreactor/fermenter combinationsBiological substance pretreatmentsHl 60 cellUltraviolet lights
On a theoretical basis that ultraviolet light (UV) and cobalt chloride simulate low oxygen to induce cell apoptosis, the research uses CoCl2 to simulate a low oxygen environment and uses 280 nm LED UVto irradiate an acute promyelocytic leukemia cell (HL-60 cell), probes into proliferation situation of the HL-60 cell after the HL-60 cell is irradiated by the 280 nm LED UV under the low oxygen environment, discovers that both the 280 nm LED UV and low oxygen inhibit proliferation of the HL-60 cell and induce the cell apoptosis, and the 280 nm LED UV has more obvious proliferation inhibition andapoptosis effects on the cell under the low oxygen condition compared with a normoxic condition. Through irradiating a transplant by the 280 nm LED UV under the low oxygen condition, remaining tumorcells in the transplant are led to apoptosis and necrosis, normal cells can survive normally, so that the ultimate purpose of eliminating remaining tumors is achieved, recurrence rate of leukemia patients after autotransplantation is reduced, and application prospect of the 280 nm LED UV in leukemia autotransplantation purging in vitro is indicated.
Owner:THE AFFILIATED HOSPITAL OF QINGDAO UNIV
Naphthalimide compound fluorescence probe for identifying Ag+ in HL-60 and its preparation method
InactiveCN107915729AHigh sensitivityHigh responseOrganic chemistryFluorescence/phosphorescenceHl 60 cellFluorescence
The invention discloses a naphthalimide compound fluorescence probe for identifying Ag+ in HL-60 and its preparation method. The naphthalimide compound with a structure of a formula (I) is applied tothe Ag+ detection in the HL-60 cell, and has high Ag+ sensitivity and response value; when it is used as the fluorescence probe, the fluorescence probe has obvious response for Ag+; when Ag+ with different concentrations is detected, the fluorescence probe shows good linear relationship; the fluorescence probe also has good selectivity of Ag+ in the HL-60 cell; the HL-60 suspending cell dyed withnaphthalimide compound is activated by blue light; through observation in a microscope, the cell emits green fluorescence, and the detection method is reliable.
Owner:FUJIAN MEDICAL UNIV
Antituberculosis compositions of byttneria species
Active fractions exhibiting anti-tuberculous activity from the plant Byttneria herbecea (family—Sterculiaceae) are described. Methanol extracts of the aerial part of Byttneria herbecea exhibited activity against MtbGS in primary screening of biosynthetic assay. Subsequent fractionation was carried out in organic solvents. Out of all these, two fractions (fraction D and K) are exhibiting inhibitory activity against M. tuberculosis glutamine synthetase activity. These fractions D and K are inhibiting 74% and 44% respectively at 7.5 mg / ml concentrations. The determined IC50 value of the fraction K is found to be 4.5 mg / ml from the dose response curve against the purified Mycobacterium tuberculosis Glutamine Synthetase. The IC50 value for M. bovis BCG is 1.56 μg / ml. It was observed that there was no significant effect on proliferation of HL-60 cell line at 10×MIC levels of the hits. This data indicated that these compounds could be initially considered to be safe.
Owner:COUNCIL OF SCI & IND RES
Novel application of Java brucea fruit oil emulsion injection to anticancer aspect
ActiveCN101879202ALow cytotoxicitySmall toxicityEmulsion deliveryAntineoplastic agentsHl 60 cellOil emulsion
Owner:SHENYANG PHARMA UNIVERSITY
Human leukemia hl-60 cell drug-resistant cell line hl-60/rs cell and preparation method thereof
InactiveCN104004715BBroad spectrum drug resistanceStable drug resistanceMicroorganism based processesTumor/cancer cellsHigh resistanceHl 60 cell
The invention discloses the drug-resistant cell line HL-60 / RS of human leukemia HL-60 cells. The preservation number of the cell line HL-60 / RS is CCTCC NO: C201430, and provides its preparation method. The HL‑60 cell line is the parental cell, and the acquired leukemia multidrug resistant cell line HL‑60 / RS. The beneficial effect of the present invention is: the leukemia multidrug-resistant HL-60 / RS cell line induced by the present invention adopts the induction method of "gradually increasing the concentration shock + continuous induction", and the experiment proves that the established drug-resistant cell line has It has the characteristics of wide drug resistance spectrum and stable drug resistance; and has high drug resistance to arsenic trioxide, which can be used to study the mechanism of sensitivity, drug resistance and arsenic resistance of tumor cells to anticancer drugs, as well as to screen reliable Drug resistance reversal agents.
Owner:LANZHOU UNIVERSITY
Antituberculosis compositions of byttneria species
In the present study, active fractions exhibiting abti-tuberculous activity from the plant Byttneria herbecea (family - Sterculiaceae) is discussed. Methanol extracts of the aerial part of Byttneria herbecea exhibited activity against MtbGS in primary screening of biosynthetic assay. Subsequent fractionation was carried out in organic solvents. Out of all these, two fractions (fraction D and K) are exhibiting inhibitory activity against M.tuberculosis glutamine synthetase activity. These fractions D and K are inhibiting 74% and 44% respectively at 7.5mg / ml concentrations. The determined IC50 value of the fraction K is found to be 4.5 mg / ml from the dose response curve against the purified Mycobacterium tuberculosis Glutamine Synthetase. The major advantage of the present study is that the mode of action of the compound is known, which increases the potential of Byttneria herbecea for becoming an anti-tubercular herbal drug. The IC50 value for M. bovis BCG is 1.56 [mu]g / ml. It was observed that there was no significant effect on proliferation of HL-60 cell line at 10 X MIC levels of the hits. This data indicated that these compounds could be initially considered to be safe.
Owner:COUNCIL OF SCI & IND RES
Traditional Chinese medicine extract composition and application thereof to preparation of medicines for leukemia treatment
InactiveCN105362664APrevent proliferationInhibit apoptosisPill deliveryGranular deliveryHl 60 cellAlcohol
The invention discloses a traditional Chinese medicine extract composition and application thereof to preparation of medicines for leukemia treatment. The traditional Chinese medicine extract composition is prepared from herba scutellariae barbatae, radix polygonati officinalis, Juncus effusus and stigma croci by steps of processing, steam distillation, alcohol extraction and macroporous resin absorption. The traditional Chinese medicine extract composition serves as an active ingredient of a pharmaceutical composition accepting additives pharmaceutically to treat leukemia. The traditional Chinese medicine extract composition is purified by distillation, alcohol extraction and macroporous resin absorption, leukemia treatment extract preparation is researched, and extracts with leukemia treatment activity are obtained by separation and prepared for leukemia treatment. The traditional Chinese medicine extract composition is capable of inhibiting proliferation of leukemia cells (HL-60 cells) and inducing apoptosis of the leukemia cells (HL-60 cells), so that the traditional Chinese medicine extract composition can be used for leukemia treatment.
Owner:YUYAO WANZHEN HARDWARE FACTORY
Method for measuring mpo halogenase activity
ActiveCN106290190BPreparing sample for investigationColor/spectral properties measurementsSerum igeHalogenase activity
A method for measuring MPO halogenase activity, the purpose of the present invention is to separate MPO with natural protein structure and enzymatic activity from HL-60 cells by ion chromatography, establish a method for measuring MPO halogenase activity, and use purified MPO as calibration The product was used to measure MPO halogenase activity units in human serum / plasma. The MPO halogenase assayed in the present invention is isolated from HL-60 cells. The present invention establishes the NaI-TMB (3,3',5,5'-tetramethylbenzidine, tetramethylbenzidine) method to directly measure the MPO halogenase activity in serum / plasma, and is used to predict the possibility and disease occurrence of cardiovascular and cerebrovascular diseases Prognosis.
Owner:CHANGCHUN HENGXIAO BIOTECH CO LTD
Application of Soimycin A in preparation of leukemia radiation and chemotherapy sensitizer
ActiveCN105902545AImprove radiosensitivityOrganic active ingredientsAntineoplastic agentsHl 60 cellObserved Survival
The invention relates to application of Soimycin A in preparation of an acute myeloid leukemia (AML) radiation and chemotherapy sensitizer. According to the application, in vitro and animal experiments show that the Soimycin A has an effect of improving the radiosensitivity of an AML cell line HL-60 and a chromicmyeloid leukemia (CML) cell line K562, and prove that the Soimycin A has an effect of improving the survival rate of AML mice constructed by HL-60 cell transplantation after chemotherapy. The sensitizer disclosed by the invention has a good application prospect for AML radiation and chemotherapy.
Owner:THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
Polyketone compound as well as preparation method and application thereof
ActiveCN106032368ANovel structureGrowth inhibitionOrganic active ingredientsOrganic chemistryAntileukemic agentHl 60 cell
The invention belongs to the field of medicine technology, and relates to a polyketone compound with antitumor activity which is separated from a strain of Ascotricha sp. and a preparation method thereof. The compound has a novel structure, and has substantial effects for inhibiting growth of a human acute promyelocytic leukemia HL-60 cell line and a human leukemia K562 cell line; a fermentation process as well as an extracting and separation method are simple, which are good for carrying out further pharmacological and clinic researches, and developing applications to preparation of medicaments for preventing and treating leukemia.
Owner:SHENYANG PHARMA UNIVERSITY
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