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Detection method for effects of inhibitor GANT61 on multiplication and apoptosis of HL-60

A detection method and inhibitor technology, applied in the medical field, can solve problems such as unclear action and mechanism

Inactive Publication Date: 2017-05-17
SHIHEZI UNIVERSITY
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Problems solved by technology

[0005] The object of the present invention is to provide a kind of GANT61 inhibitor to the detection method of HL-60 proliferation and apoptosis, aim to solve the Hedgehog (HH) signaling pathway inhibitor GANT61 with GLI as the target on human acute promyelocytic leukemia cells The role and mechanism of HL-60 proliferation and apoptosis are not clear

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  • Detection method for effects of inhibitor GANT61 on multiplication and apoptosis of HL-60

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[0024] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0025]In the present invention, GANT61, a Hedgehog-GLI signaling pathway-specific inhibitor targeting GLI, is used to inhibit the expression of the Hedgehog-GLI pathway, and to observe the proliferation and apoptosis of acute myeloid leukemia cells after the Hedgehog-GLI pathway is inhibited. The treatment of leukemia offers new therapeutic strategies.

[0026] The GANT61 inhibitor provided by the specific embodiment of the present invention,

[0027] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0028] 1 Materials and methods

[0029] 1.1 ...

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Abstract

The invention discloses a detection method for effects of an inhibitor GANT61 on multiplication and apoptosis of HL-60. The detection method comprises the following steps: detecting the growth and multiplication conditions of HL-60 cells with inhibitors of different concentrations through a CCK-8 (Cell Counting Kit-8) method, detecting the apoptosis condition of the HL-60 cells with an inhibitor through double staining, detecting the expression condition of the HL-60 cells with inhibitors of different concentrations through RT-PCR, and detecting the expression condition of proteins of the HL-60 cells with inhibitors of different concentrations through an immunofluorescence technology. According to the detection method, expression of a Hedgehog-GLI passageway is inhibited by a Hedgehog-GLI signal passageway specific inhibitor taking GLI as a target, and when the Hedgehog-GLI passageway is inhibited, the multiplication and apoptosis conditions of acute myeloid leukemia cells are observed; and a new treatment strategy is supplied to treatment of leucocythemia.

Description

technical field [0001] The invention belongs to the field of medical technology, and in particular relates to a detection method for HL-60 proliferation and apoptosis by GANT61 inhibitors. Background technique [0002] Acute myeloid leukemia (AML) is a malignant clonal disease of hematopoietic stem cells. Although the current induction therapy has a complete remission (CR) rate of nearly 80%, more than 60% eventually relapse due to drug resistance. It is high-dose chemotherapy and stem cell transplantation, which still has a high recurrence rate, and the 5-year overall survival rate is less than 40%. Therefore, it is very necessary to develop new therapeutic drugs and new combined treatment regimens. [0003] In recent years, studies have found that abnormal activation of the HH signaling pathway is related to the occurrence and development of various tumors, including leukemia, and is involved in tumor angiogenesis, regulation of tumor stem cells, and tumor invasion and me...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/68G01N33/574C12Q1/02C12Q1/68
CPCG01N33/5008C12N2503/00C12Q1/6886C12Q2600/158G01N33/57426G01N33/6893G01N2800/52G01N2800/54G01N2800/7028
Inventor 魏虹邓磊
Owner SHIHEZI UNIVERSITY
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