Method for measuring mpo halogenase activity

A technology of halogenase activity and halogenase, which is applied in the field of natural protease purification, can solve the problems of long time, long time required, high preparation cost, etc., and achieve the effect of strong specificity
CN106290190BActive Publication Date: 2019-01-25CHANGCHUN HENGXIAO BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHANGCHUN HENGXIAO BIOTECH CO LTD
Publication Date
2019-01-25

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Abstract

A method for measuring MPO halogenase activity, the purpose of the present invention is to separate MPO with natural protein structure and enzymatic activity from HL-60 cells by ion chromatography, establish a method for measuring MPO halogenase activity, and use purified MPO as calibration The product was used to measure MPO halogenase activity units in human serum / plasma. The MPO halogenase assayed in the present invention is isolated from HL-60 cells. The present invention establishes the NaI-TMB (3,3',5,5'-tetramethylbenzidine, tetramethylbenzidine) method to directly measure the MPO halogenase activity in serum / plasma, and is used to predict the possibility and disease occurrence of cardiovascular and cerebrovascular diseases Prognosis.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, in particular to a method for purifying natural protease. Background technique

[0002] Myeloperoxidase (Myeloperoxidase, MPO), also known as peroxidase, is a glycosylated heme protease secreted by neutrophils, monocytes and macrophages in some tissues , is a member of the heme peroxidase superfamily. Before human granulocytes enter the circulation, MPO is synthesized in the bone marrow and stored in azurophilic granules. External stimuli can cause neutrophils to gather and release MPO. In mature granulocytes (mainly neutrophils and monocytes), MPO is the most abundant glycoprotein, accounting for about 5% of the total protein content in peripheral blood polymorphonuclear neutrophils (PMNs), 95% of MPO in blood originates from PMNs

[0003] Myeloperoxidase (MPO) is a dimer composed of two subunits, each subunit consists of a heavy chain (α chain, 466 amino acid residues) and a light chain (β chain...

Claims

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