Method of utilizing tribonema to comprehensively refine biological product, biological diesel and biological material

A technology of biodiesel and yellow algae, applied in the direction of microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problems of unrealistic, restricting the commercial development of third-generation microalgae bioenergy, and high production costs

Active Publication Date: 2019-01-01
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to technical limitations, the main focus is on the screening of high-quality algae strains with high oil yields in the upstream process, efficient large-scale cultivation of microalgae, and energy-saving harvesting technology, which makes the production cost much higher than the current fossil fuel

Method used

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  • Method of utilizing tribonema to comprehensively refine biological product, biological diesel and biological material
  • Method of utilizing tribonema to comprehensively refine biological product, biological diesel and biological material
  • Method of utilizing tribonema to comprehensively refine biological product, biological diesel and biological material

Examples

Experimental program
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Effect test

Embodiment 1

[0067] Embodiment 1: Filamentous microalgae - yellow silk algae (Tribonema sp.) culture

[0068] Inoculate the filamentous microalgae-Tribonema sp. algae preserved in the triangular flask into a columnar glass photobioreactor (length×inner diameter=60×6cm) (purchased from Guangzhou Genshui Experimental Equipment Co., Ltd.) Expansion and cultivation. The culture conditions are: mBG-11 liquid medium, temperature 25±1℃, continuous unilateral incandescent light (80~90μmol / m 2 / s), containing 1% CO 2 Compressed air blowing culture for 6 to 7 days. After the expansion cultivation, filter and harvest the algae through a sieve (300 mesh); The base was mBG-11 liquid medium, the initial concentration of sodium nitrate was set to 18mM, and the continuous unilateral concentration was 250μmol / m 2 / s light, other culture conditions are the same as above. After culturing for 15 days, measure the biomass, and filter through a sieve (300 mesh, pore size 48 μm) to harvest the wet algae mud...

Embodiment 2

[0070] Embodiment 2: Extraction and separation of Chrysophylla laminarum sugar

[0071] Weigh a certain amount of dry algal powder obtained in Example 1 into a flask, and then suspend in 50 mM dilute sulfuric acid. The flask was placed in a constant temperature water bath at 80°C, and an electric stirrer was used to continuously stir and extract at 200rpm for 4h. After the extraction, the residual algae and the supernatant were separated by filtration through a silk sieve (300 mesh, pore size 48 μm), and the residual algae was extracted once more. The supernatants were combined, and 95% ethanol was added according to the ratio of v / v=1:4, and alcohol precipitation was carried out at 4°C for 12 hours. The harvested residual algae were washed with water and then dried in an oven at 60°C to obtain desugared algae residue, which was used for the extraction of total lipids. The alcohol-precipitated precipitate was obtained by centrifugation at 7500 rpm for 10 min, washed with eth...

Embodiment 3

[0073] Example 3: Extraction and enrichment of palmitoleic acid

[0074] (1) Extraction of total fat: after mixing the algae residue after sugar extraction obtained in Example 2 with 95% ethanol: n-hexane (v / v=1:1) according to the ratio of m / v=1:25, place Sonicate in an ultrasonic cleaner for 2 h. After ultrasonic treatment, the flask containing the mixed solution was placed in a constant temperature water bath at 50° C., and extracted with continuous stirring (200 rpm) by an electric stirrer for 6 h. After the extraction, filter the remaining algae residue through a sieve. Repeat the above step 3 to 4 times again until the algae residue turns off-white, and after the harvested supernatants are combined, remove the organic reagent in a rotary evaporator (50° C.) to obtain the total lipid extract. Detected by gas chromatography, the fatty acid composition in the total fat is mainly myristic acid (C14:0), palmitic acid (C16:0), palmitoleic acid (C16:1), oleic acid (C18:1), ei...

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Abstract

The invention discloses a method of utilizing tribonema to comprehensively refine a biological product, biological diesel and a biological material. According to the method disclosed by the invention,the same biomass raw material of filiform microalgae-tribonema is utilized to jointly produce palmitoleic acid, chrysolaminaran, biological diesel and nano cellulose. The microalgae biomass resourceis comprehensively utilized, so that the biological product, biological energy and a biological nanomaterial in the microalgae industry can be better coupled, and economic effects are effectively improved. According to the method disclosed by the invention, a biological refining technical route of a high-efficiency and reasonable tribonema biomass resource is established for the first time; namely, diluted acid is utilized to treat microalgae biomass at first to extract intercellular polysaccharide; furthermore, cell wall structures can be loosened, the next step of grease extraction is more facilitated, and finally residual algae dregs can be fully utilized. The technical route obviously reduces production cost, improves microalgae biomass utilization efficiency and has an obvious compensation effect.

Description

technical field [0001] The patent of the present invention relates to the comprehensive refining of biomass resources of filamentous microalgae-Tribonema sp., extracting and separating active substances such as kelp sugar and palmitoleic acid from the same biomass raw material of Tribonema sp., and The technical method of preparing biodiesel and nanocellulose by using the extracted oil and the remaining algal residue respectively belongs to the fields of separation and purification of natural products of microalgae, preparation of biodiesel and bionano materials, including extraction, chromatographic column chromatography, urea coating Buried, acid-base catalyzed methyl esterification, acid hydrolysis and other related technologies. Background technique [0002] At present, microalgae biofuel is considered to be a new, renewable and sustainable clean energy that is most likely to replace traditional fossil fuels. However, due to technical limitations, the main focus is on t...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P19/04C12R1/89
CPCC12P7/6409C12P7/649C12P19/04Y02E50/10
Inventor 张成武王飞飞高保燕黄罗冬
Owner JINAN UNIVERSITY
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