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Method for extracting palmitoleic acid from sea buckthorn fruit oil

A technology of seabuckthorn fruit oil and palmitoleic acid, which is applied in the field of extraction and purification of natural products, can solve problems such as no patent publications have been found, difficulties in meeting social needs, and palmitoleic acid production limitations, so as to improve the utilization value and increase Yield, method simple effect

Inactive Publication Date: 2016-10-12
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the international ban on whaling and the lack of fishery resources, the production of palm oleic acid is greatly restricted, and it is difficult to meet the needs of the society. The research on its production process has good economic value
[0003] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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  • Method for extracting palmitoleic acid from sea buckthorn fruit oil
  • Method for extracting palmitoleic acid from sea buckthorn fruit oil
  • Method for extracting palmitoleic acid from sea buckthorn fruit oil

Examples

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Effect test

Embodiment 1

[0042] A method for extracting palmitoleic acid from seabuckthorn fruit oil, the steps are as follows:

[0043] (1) Saponification of the seabuckthorn fruit oil sample: the seabuckthorn fruit oil sample is saponified using a conventional saponification method, so that the fatty acids in the sample are in a free state, and the saponified seabuckthorn fruit oil mixed fatty acid is obtained;

[0044] (2) Urea embedding: After mixing urea and common organic solvents in proportion, stir and reflux at 80°C until the urea is completely dissolved, then add seabuckthorn fruit oil mixed fatty acids, mix well, reflux at 80°C for 2 hours, and cool to room temperature; After embedding the crystals at 4-20°C for 6-30 hours, quickly suction filter to obtain the filtrate and filter residue. After removing ethanol from the filtrate, use warm water or acid solution to break the urea to obtain fatty acid samples. Wash the filter residue with warm water to remove urea and recover the saturated Fa...

Embodiment 2

[0049]A method for extracting palmitoleic acid from seabuckthorn fruit oil, the steps are as follows:

[0050] (1) Saponification of the seabuckthorn fruit oil sample: the seabuckthorn fruit oil sample is saponified using a conventional saponification method, so that the fatty acids in the sample are in a free state, and the saponified seabuckthorn fruit oil mixed fatty acid can be obtained, specifically as follows:

[0051] Get 20g seabuckthorn fruit oil sample, place in 250mL flat-bottomed flask, then add ethanol and KOH and place it on a constant temperature water bath, the volume percentage is 95% ethanol: the alcohol-oil ratio mL:g of seabuckthorn fruit oil is 2:1, so The mass ratio of KOH to seabuckthorn fruit oil is 1:1, turn on the magnetic stirring, reflux at a temperature of 70°C for 2 hours, pour an appropriate amount of distilled water (water: seabuckthorn fruit oil ratio 2:1), and rotary evaporate the saponified solution to recover ethanol. Extract unsaponifiable ...

Embodiment 3

[0057] A method for extracting palmitoleic acid from seabuckthorn fruit oil, the steps are as follows:

[0058] ⑴Saponification of seabuckthorn fruit oil

[0059] Get 20g seabuckthorn fruit oil sample, place in 250mL flat-bottomed flask, then add ethanol and KOH and place it on a constant temperature water bath, the volume percentage is 95% ethanol: the alcohol-oil ratio mL:g of seabuckthorn fruit oil is 2:1, so The mass ratio of KOH to seabuckthorn fruit oil is 1.1:1, turn on the magnetic stirring, reflux at a temperature of 90°C for 2 hours, pour an appropriate amount of distilled water (the ratio of water: seabuckthorn fruit oil is 2:1), and obtain a saponified solution, which is saponified by rotary evaporation Ethanol is recovered from the liquid, and unsaponifiables are extracted with a small amount of petroleum ether;

[0060] Take an equal volume of distilled water from the water layer, add 6 mol / L hydrochloric acid dropwise under agitation to acidify to pH=2-3, pour ...

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Abstract

The invention relates to a method for extracting palmitoleic acid from sea buckthorn fruit oil. The method comprises the following steps that saponification treatment is conducted on a sea buckthorn fruit oil sample, and saponified sea buckthorn fruit oil mixed fatty acid is obtained; urea and a commonly-used organic solvent are mixed and subjected to stirring refluxing to enable urea to be completely dissolved, then the sea buckthorn fruit oil mixed fatty acid is added, refluxing extraction is conducted for 30 min to 120 min, and cooling is conducted to room temperature; suction filtration is conducted rapidly after embedding crystallization is conducted, the commonly-used organic solvent in filtrate is removed, urea in the filtrate is destroyed, and a fatty acid sample is obtained; the fatty acid sample obtained after urea embedding is conducted is treated, a light component with the average free path length is separated on the conditions that the pressure ranges from 0.1 Pa to 0.2 Pa, the distillation temperature ranges from 70 DEG C to 140 DEG C, and the feeding speed and the film scraping rotating speed are guaranteed to form homogeneous films, and palmitoleic acid is obtained. According to the method for extracting palmitoleic acid from the sea buckthorn fruit oil, the method is simple and convenient to operate, the content of obtained palmitoleic acid exceeds 80% of the total mass of the product, the yield of palmitoleic acid can be greatly increased, and the vacancy of domestic palmitoleic acid products is filled up.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of natural products, in particular to a method for extracting palmitoleic acid from seabuckthorn fruit oil. Background technique [0002] In recent years, palmitoleic acid has attracted a lot of attention because of its therapeutic role in some everyday diseases, such as metabolic syndrome, diabetes and some inflammations. Palmitoleic acid is a 16-carbon monounsaturated fatty acid (monounsaturated fatty acid, MUFA), containing only one double bond, located on the seventh carbon atom at the carbon end (16:1n-7). At present, palm oleic acid is mainly derived from seafood such as whale oil and cod liver oil. Due to the international ban on whaling and the lack of fishery resources, the production of palm oleic acid is greatly restricted, and it is difficult to meet the needs of the society. The research on its production process has good economic value. [0003] Through searchin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11C3/00C11C1/10
CPCC11C3/00C11C1/10
Inventor 张泽生高山
Owner TIANJIN UNIV OF SCI & TECH
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