Specific-antigen preparing method used for detecting schistosoma japonicum antibodies by indirect hemagglutination (IHA) method

Abstract

The invention provides a specific-antigen preparing method used for detecting schistosoma japonicum antibodies by an indirect hemagglutination (IHA) method. The method includes the following steps: step one, preparing schistosoma-specific antigens, wherein in a step 1, soluble adult antigens of schistosoma japonicum are prepared, in a step 2, soluble cercaria antigens of the schistosoma japonicumare prepared, in a step 3, soluble egg antigens of the schistosoma japonicum are prepared, in a step 4, the antigens are treated by sodium periodate, and in a step 5, the soluble adult antigens of theschistosoma japonicum, the soluble cercariae antigens of the schistosoma japonicum and the soluble egg antigens of the schistosoma japonicum after treating of the sodium periodate are proportionallymixed according to a volume ratio of 1:0.5:2, that is, specific antigens are obtained; and step two, preparing an indirect hemagglutination reagent by the specific antigens. According to the method, the crudely prepared soluble antigens of schistosoma japonicum adults, cercariae and eggs are proportioned and mixed after treating of the sodium periodate, the diagnostic reagent is prepared, and thereagent reduces cross-reaction with other diseases, and maintains original high sensitivity and specificity at the same time.

Description

technical field

[0001] The invention relates to the technical field of schistosome antibody detection, in particular to a method for preparing a specific antigen used in the detection of schistosome antibody in an indirect hemagglutination reagent. Background technique

[0002] Schistosomiasis is a major infectious disease that seriously endangers the health of the population and hinders economic and social development. The party and government of our country attach great importance to the prevention and control of schistosomiasis. After more than 60 years of hard work, the prevention and control of schistosomiasis in my country has achieved world-renowned achievements. However, in the process of controlling and eliminating schistosomiasis, we still have many technical barriers and obstacles. Especially with the in-depth prevention and control of schistosomiasis, the infection rate of schistosomiasis in endemic areas is getting lower and lower, and the infection degree is ge...