Colloidal gold chromatography test strip for detecting skeletal muscle troponin I of cattle or sheep, and application thereof

An immunochromatographic test strip, troponin technology, applied in biological testing, measuring devices, analytical materials, etc., can solve the problems of protein denaturation, loss of antigenicity and water solubility, and difficulty in immunological methods, achieving low cost and easy access. The effect of promoting the use, the method is simple
CN109709339AActive Publication Date: 2019-05-03BIOLOGY INST OF HEBEI ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BIOLOGY INST OF HEBEI ACAD OF SCI
Publication Date
2019-05-03

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Abstract

The invention, which belongs to the technical fields of immunology and food safety analysis, relates to a colloidal gold chromatography test strip for detecting skeletal muscle troponin I of cattle orsheep, and a preparation method and application thereof. The test strip comprises a bottom plate, a sample pad, a colloidal gold pad, a coating film and an absorbent pad. Protective films are arranged at two ends of the test strip. The sample pad, the colloidal gold pad, the coating film and the absorption pad are pasted on the bottom plate successively. The colloidal gold pad is coated with a colloidal-gold-labeled capture antibody. The coating film is provided with an invisible detection line printed by a detecton antibody solution and an invisible quality control line printed by a goat anti-mouse IgG solution. The capture antibody is obtained by secretion of a hybridoma cell strain 3A8 having the preservation number of CCTCC NO:C2018217; and the detection antibody is obtained by secretion of a hybridoma cell strain with the preservation number of CCTCC NO:C2018218. The colloidal gold chromatography test strip having characteristics of great convenience, rapidity, visibility, strongtimeliness, wide application range, and low cost is convenient to promote and apply.
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Description

technical field

[0001] The invention relates to a colloidal gold immunochromatographic test strip for detecting bovine or sheep skeletal muscle troponin I and a preparation method and application thereof, which belong to the technical field of immunology and food safety analysis. Background technique

[0002] In meat products, due to reasons such as price, religion, and health, many countries have enacted regulations requiring food labels to truly and clearly indicate the source of meat and prohibit adulteration to protect the interests of consumers. However, the confusion of meat varieties in the market The phenomenon is still very common. Adulteration methods include blending, mixing, extraction, counterfeiting, etc., especially the adulteration and adulteration of beef and mutton products are the most common, and these adulteration behaviors have greatly damaged the interests of consumers. .

[0003] At present, many laboratories at home and abroad are using molecular bi...

Claims

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