Human immunodeficiency virus (HIV) antibody time resolved fluoroimmunoassay method and kit

A human immunodeficiency, time-resolved technology, applied in the field of human immunodeficiency virus HIV antibody detection kits, can solve the problems of polluted environment, low sensitivity, cumbersome operation, etc., and achieve small cross-reaction, good specificity, and short detection time Effect

Inactive Publication Date: 2011-11-09
PERKINELMER MEDICAL DIAGNOSTICS PROD SHANGHAI
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a time-resolved immunofluorescence analysis method and kit for human immunodeficiency virus HIV (type 1+2) antibody detection, which mainly solves the problems of low sensitivity, poor stability, cumbersome operation and problems in the prior art. Environmental pollution and other technical issues

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  • Human immunodeficiency virus (HIV) antibody time resolved fluoroimmunoassay method and kit
  • Human immunodeficiency virus (HIV) antibody time resolved fluoroimmunoassay method and kit
  • Human immunodeficiency virus (HIV) antibody time resolved fluoroimmunoassay method and kit

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Embodiment

[0020] Embodiment: human immunodeficiency virus HIV (type 1+2) antibody detection

[0021] 1. Before performing time-resolved immunofluorescence assay, the following preparatory work should be completed:

[0022] First prepare coated plate, used coated plate is the plate coated with specific recombinant antigen, and the preparation of HIV coated plate comprises the following steps:

[0023] (1) Dilute the purified HIV recombinant antigen with carbonate buffer, then add it to each well of the coated strip, and obtain the HIV recombinant antigen coated strip after adsorption, washing, sealing and drying;

[0024] (2) Put the HIV recombinant antigen-coated slat into a special aluminum foil packaging bag, seal it and refrigerate it for later use. Next is the preparation of the HIV recombinant antigen europium label, including the following steps:

[0025] (a) Add the antigen to be labeled into the dialysis bag, put it into the prepared carbonate buffer solution of pH 9.5, and di...

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Abstract

The invention discloses a human immunodeficiency virus (HIV) (type 1+2) antibody detection double-antigen sandwich time resolved fluoroimmunoassay method and a kit. Recombinant niobium is used as a coating antigen, and sodium carbonate buffer solution is diluted to 1 to 10mu g / mL to form coating solution; paired HIV recombinant antigens are labeled as labeled antigens by using lanthanide elements; in an experiment, the reaction buffer solution is diluted for use in a ratio of 1:21; and 100mu L of HIV negative or positive control or sample to be detected and the diluted labeled antigens are sequentially added into each pore of a reaction plate of coating antibodies, and fluorescence detection is performed after incubation. The invention also provides the corresponding kit. The kit has relatively high sensitivity, specificity and stability; and because the analysis system has high automation, the speed of the clinical inspection result can be improved, human errors can be greatly reduced, and the reliability of the detection result is increased.

Description

Technical field: [0001] The invention relates to a human immunodeficiency virus antibody time-resolved immunofluorescence analysis method and a kit, in particular to a human immunodeficiency virus HIV (type 1+2) antibody detection kit. Background technique: [0002] AIDS (AIDS) is the abbreviation of Acquired Immune Deficiency Syndrome, that is, "acquired (acquired) immune deficiency syndrome". AIDS is caused by HIV, also known as Human Immunodeficiency Virus (Human Immunodeficiency Virus), HIV for short. In the past 20 years since it was first discovered in the United States in 1981, it has become very popular all over the world, causing very serious harm. According to the "Joint Assessment Report on AIDS Prevention and Control in China (2007)", by the end of October 2007, a total of 223,501 HIV-infected and AIDS patients had been reported nationwide, including 62,838 AIDS patients and 22,205 deaths. [0003] According to the 2007 AIDS Epidemic Update jointly released by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/533
Inventor 张小寒
Owner PERKINELMER MEDICAL DIAGNOSTICS PROD SHANGHAI
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