Human immunodeficiency virus (HIV) antibody time resolved fluoroimmunoassay method and kit
A human immunodeficiency, time-resolved technology, applied in the field of human immunodeficiency virus HIV antibody detection kits, can solve the problems of polluted environment, low sensitivity, cumbersome operation, etc., and achieve small cross-reaction, good specificity, and short detection time Effect
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[0020] Embodiment: human immunodeficiency virus HIV (type 1+2) antibody detection
[0021] 1. Before performing time-resolved immunofluorescence assay, the following preparatory work should be completed:
[0022] First prepare coated plate, used coated plate is the plate coated with specific recombinant antigen, and the preparation of HIV coated plate comprises the following steps:
[0023] (1) Dilute the purified HIV recombinant antigen with carbonate buffer, then add it to each well of the coated strip, and obtain the HIV recombinant antigen coated strip after adsorption, washing, sealing and drying;
[0024] (2) Put the HIV recombinant antigen-coated slat into a special aluminum foil packaging bag, seal it and refrigerate it for later use. Next is the preparation of the HIV recombinant antigen europium label, including the following steps:
[0025] (a) Add the antigen to be labeled into the dialysis bag, put it into the prepared carbonate buffer solution of pH 9.5, and di...
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