Preparation method and application of magnetic graphene oxide-nanogold label-free complex

A nano-gold, label-free technology, used in biochemical equipment and methods, DNA preparation, and microbial assay/inspection to reduce cross-reactions

Pending Publication Date: 2016-08-24
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no report on the detection of small RNA by combining magnetic graphene oxide with non-sulfhydryl nucleic acid to prepare magnetic graphene oxide-nano-gold label-free complex

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  • Preparation method and application of magnetic graphene oxide-nanogold label-free complex

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Embodiment 1

[0049] 1. Experimental principle:

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Abstract

The invention discloses a preparation method and an application of a magnetic graphene oxide-nanogold label-free complex. On the basis of different adsorption effects of magnetic graphene oxide on single-stranded DNA and double-stranded DNA, after small target RNA is added, an identification section of DNA1 is specifically bound to the small target RNA, so that a DNA / RNA double-stranded structure is formed and a gold nanoprobe, which is adsorbed on the surface of the magnetic graphene oxide, is dissociated in a solution. Through magnetic separation, on the basis of a developing effect of nanogold in a visible light region, the color change of the nanogold solution in a supernatant, along with different small RNA concentrations, is observed; and through quantitative analysis by virtue of an ultraviolet-visible spectrophotometer, rapid colorimetric detection, with high sensitivity and high specificity, on the small RNA is achieved.

Description

technical field [0001] The invention relates to a small RNA detection method, in particular to a preparation method and application of a magnetic graphene oxide-nano gold label-free complex. Background technique [0002] Colorimetry is an analytical method that uses the color depth of a colored substance solution to determine the content of the substance to be measured. It has the advantages of simple operation, high sensitivity, and high accuracy, and has been widely used in the field of biochemical analysis. Among them, gold nanoparticles have shown potential application value in the field of biological detection due to their unique optical properties, good biocompatibility and chemical activity. At present, most of the work using nano-gold colorimetric method to detect nucleic acid molecules is to self-assemble sulfhydryl-modified DNA onto the surface of gold nanoparticles through Au-S covalent bonds. These methods require chemical modification of DNA molecules, which ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6823C12Q2563/137C12Q2525/173C12Q2525/207
Inventor 毕赛岳淑珍王宗花张立学龚世达
Owner QINGDAO UNIV
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