Application of let-7b and rSjp40 (recombinant Schistosoma japonicum p40) in preparation of drug for preventing or treating schistosoma infected hepatic fibrosis

Abstract

The invention provides application of let-7b and rSjp40 (recombinant Schistosoma japonicum p40) in preparation of a drug for preventing or treating schistosoma infected hepatic fibrosis. The application is mainly shown as follows: (1) rSjp40 up-regulates expression of let-7b and promoter thereof in a human LX-2 cell line; (2) let-7b participates in the activation process of inhibiting human hepatic stellate cells (HSCs) by the rSjp40; and (3) the rSjp40 can promote increase of expression of let-7b in hepatic tissue and is in negative correlation with expression of collagen. By the function andmolecular mechanisms of rSjp40 in inhibiting the let-7b in the activation process of the HSCs, schistosoma infected hepatic fibrosis is inhibited at the gene level, the application of the let-7b andrSjp40 in preparation of the drug for preventing or treating schistosoma infected hepatic fibrosis is obtained, and the problem of schistosoma infected hepatic fibrosis is expected to be solved in thefuture.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and specifically relates to the application of let-7b and rSjp40 in the preparation of drugs for preventing or treating hepatic fibrosis infected by Schistosoma japonicum. Background technique [0002] Liver fibrosis is a reversible wound healing process aimed at maintaining organ integrity. Fibrosis is usually caused by chronic liver injury caused by various factors, mainly viral infection, schistosomiasis and alcoholism, etc. Although many drugs have been shown to have anti-fibrotic activity in vitro and in animal models, none of these drugs has been proven clinically effective. [0003] Hepatic stellate cells (HSCs) produce collagen during their initiation, progression, and regression by secreting fibrotic factors that promote portal fibroblasts, fibroblasts, and bone marrow-derived myofibroblasts, thereby forming fibrosis. Understanding the molecular mechanism of liver fibrosis and its re...

AI Technical Summary

Problems solved by technology

Although many studies have focused on the regulation of miRNAs in different pathways of fibrosis, reports on the key miRNAs regulating HSCs in the process of fibrosis have received little attention.

The Let-7 (let-7) family is composed of 13 members located on 9 different chromosomes, and its members regulate cell cycle, cell differentiation, and apoptosis; about Let-7 (let-7), SjP40 jointly targets Schistosoma japonicum infection liver No treatments or drugs for fibrosis have been studied

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