Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use

A diagnostic kit and technology for schistosomiasis, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of troublesome labeling methods, expensive colloidal gold, expensive enzymes, etc., and achieve simple labeling methods and fast testing methods , The effect of the simple test method

Inactive Publication Date: 2003-06-25
JIANGSU INST OF PARASITIC DISEASES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its disadvantages are: (1) colloidal gold is more expensive, especially the price of finished colloidal gold is particularly expensive, and it is often an imported product. Although self-made colloidal gold can reduce costs, it is difficult to obtain relatively satisfactory colloidal gold particles; (2) colloidal gold The gold labeling process is cumbersome, the conditions are not easy to grasp, and it needs to be purified before use; (3) Colloidal gold labels need to be stored at 4°C, which is not conducive to transportation
The disadvantages are: (1) the price of the enzyme is relatively expensive; (2) the labeling method is cumbersome and needs to be purified before use; (3) the enzyme is easily inactivated and needs to be stored at 4°C, which is not conducive to transportation; (4) detection It takes a long time, generally about 2 hours, and is not suitable for large-scale on-site censuses

Method used

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  • Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use
  • Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use
  • Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use

Examples

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Effect test

Embodiment 1

[0079] Example 1: According to the method described in the instruction manual, the composition of the prepared kit includes 48 test strips, 3ml of dye-labeled antigen solution, and 48 small cups of PVC standard plates.

Embodiment 2

[0080] Example 2: Detect 30 cases of acute schistosomiasis patient serum and 84 cases of chronic schistosomiasis patient serum with schistosomiasis quick test strip diagnostic kit, its sensitivity is respectively 96.7% and 94.0%, detect 60 cases of healthy person's serum, its specificity was 96.7%. Joden's index is respectively 0.967+0.967-1=0.934; 0.94+0.967-1=0.907. It shows that the method has high sensitivity and good specificity. It is an efficient immunodiagnostic kit for schistosomiasis.

[0081] ——Excerpt from pages 18 to 30 of "Chinese Journal of Schistosomiasis Prevention and Control", Volume 12, Issue 1, 2000

Embodiment 3

[0082] Embodiment 3: on-the-spot application: in 2001, 121 cases of schistosomiasis patients' serum were detected by the schistosomiasis rapid test strip diagnostic kit in Boyang County, Jiangxi Province, and the positive rate was 96.69% (117 / 121). It is negative, the specificity is 100%, and the Joden's index can reach 0.967+1.000-1=0.967. It shows that the method has high sensitivity and good specificity, and is suitable for on-the-spot investigation.

[0083] ——Excerpted from pages 28 to 30 of "Chinese Journal of Parasitic Disease Control", Volume 14, Issue 1, 2001

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Abstract

The present invention belongs to immunological parasitic disease diagnosis technology. The reagent kit is prepared via dye labeling process and includes labeling antigen solution compounded with 2BLN disperse blue dye, soluble schistosoma japoncium egg antigen, 1% sodium azide and bow serum albumen blocking liquid; and test paper strip with nitrocellulose as chromatographic film detection lines of human sheep antigen IgG and contrast lines of soluble schistosoma japonium egg resisting rabbit antigen IgG on the chromatographic film. The serum to be tested is mixed with the labeling antigen solution and the mixture liquid is tested with the test paper strip.

Description

technical field [0001] The invention relates to a quick test strip diagnostic kit for schistosomiasis and its preparation method and application. The so-called dye labeling method adopts an industrial dye instead of an enzyme as a marker, which is used for the quick test strip diagnosis of schistosomiasis, and belongs to the technical field of immunodiagnosis of parasitic diseases. Background technique [0002] At present, the schistosomiasis test strip diagnostic method close to the present invention has following two kinds: [0003] 1. Colloidal gold labeling method: tetrachloroauric acid is prepared into a solution of colloidal gold particles with a diameter of about 5-10nm by reduction (mainly white phosphorus reduction, sodium borohydride reduction and tannic acid-trisodium citrate reduction, etc.) , it is a red solution, because the surface of colloidal gold has a negative charge and the protein has a positive charge, and the two are firmly combined due to electrostati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N33/53G01N33/532G01N33/569
Inventor 朱荫昌何伟
Owner JIANGSU INST OF PARASITIC DISEASES
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