Application of near infrared xanthene fluorescent dye in fluorescent labeling of adult schistosoma

A fluorescent dye and fluorescent labeling technology, which is applied in the application field of near-infrared xanthene fluorescent dyes in the fluorescent labeling of Schistosoma adult worms, can solve the problems of long-term fluorescent observation, fluorescent interference, and poor fluorescent penetration, and achieve good results. The effect of light penetration performance and high signal-to-noise ratio
CN108344722AActive Publication Date: 2018-07-31GANNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GANNAN NORMAL UNIV
Publication Date
2018-07-31

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Abstract

The invention provides application of near infrared xanthene fluorescent dye in fluorescent labeling of adult schistosoma. The near infrared xanthene fluorescent dye has the structure shown in formulaI. In the application, the near infrared xanthene fluorescent dye (NIR-RB) with the structure of the formula I has an emission peak located in a near infrared region and has good light transmittancein living body fluorescence imaging. The NIR-RB can perform fluorescent labeling imaging on the adult schistosomiasis; meanwhile, the fluorescence intensity of the adult schistosoma is quantitativelyanalyzed, the signal-to-noise ratio (S / N) of a fluorescent signal in a worm body region and a background region is 10, which indicates that the NIR-RB used for the fluorescence imaging of the adult schistosoma has a high signal-to-noise ratio.
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Description

technical field

[0001] The invention relates to the technical field of bio-optical labeling, in particular to the application of a near-infrared xanthene fluorescent dye in the fluorescent labeling of schistosomiasis adults. Background technique

[0002] Schistosomiasis is an infectious disease that is prevalent in tropical and subtropical regions, seriously endangering people's health and affecting social and economic development. Schistosomiasis is endemic in 74 countries and regions. There are currently about 652 million people in the world at risk, 193 million infected people, and about 120 million symptomatic cases, of which 20 million are severe cases. China is the main endemic area of ​​schistosomiasis.

[0003] Fluorescence imaging can be used in the study of schistosomiasis, Andrea et al. ,113,130-133) used fluorescent probes to detect the content of nitric oxide in Schistosoma japonicum, and found that the release of nitric oxide in worms was dependent on nitric o...

Claims

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