Composite molluscacide containing ginkgolic acid and its prepn process

A technology of ginkgolic acid and molluscicide, applied in the field of biological pesticides, can solve the problems of environmental pollution, high toxicity, toxicity and the like

Inactive Publication Date: 2007-12-19
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium pentachlorophenate has been used in many countries. It has a good molluscicide effect, but it has broad-spectrum toxicity to non-target organisms, especially fish and shrimp farming, and causes serious environmental pollution. It has long been listed as a banned product by the international community
Niclosamide (Niclosamidum) has a good molluscicide effect and is the only molluscicide recommended by the World Health Organization, but it is expensive and its use in large areas in developing countries is limited; it is highly toxic to aquatic organisms such as fish and is considered Prohibition of use in aquaculture waters with aquatic organisms, thus limiting the scope of application
Moreover, it cannot effectively prevent the snails from climbing up to escape killing, and it can also lead to increased drug resistance of target organisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1). Preparation of ginkgolic acid total extract and its monomers:

[0036] Add petroleum ether (boiling range 60-90°C) to extract 100g of dried and crushed Ginkgo exocarp in a ratio of 1:5 for 1 hour, filter, and repeat the extraction of the filter residue twice, combine the extractions, and evaporate the petroleum ether to obtain the exocarp. Skin crude extract extract.

[0037] Dissolve the extract with a small amount of petroleum ether, load the sample on a silica gel column (the silica gel column is a Φ32×400mm glass column, wet-packed), use petroleum ether: ether: formic acid = 89: 11: 1 (V / V / V) Elution, taking the fluorescence at 254 nm in thin layer chromatography as the detection index. Collect the ginkgolic acid total extract components, concentrate to dryness; repeat the column once, the obtained ginkgolic acid total extract components are washed to neutrality, and concentrated to dryness to obtain the ginkgolic acid total extracts.

[0038] A preparative C...

Embodiment 2

[0042] Dissolve the total ginkgolic acid extract with a small amount of absolute ethanol first, add an appropriate emulsifier, and emulsify at a high speed to prepare an emulsion, and then dilute it with boiled and cooled tap water to different concentrations: 40mg / L, 20mg / L, 10mg / L , 5mg / L, 1.25mg / L (the final concentration of ethanol is <0.1%). Simultaneously, set the tap water cooled after boiling as blank control, and dissolve this tap water (250ug / l) with niclosamide wettable powder as positive control drug.

[0043] The molluscicide test was carried out according to the immersion method in the WHO "Molluscicide Laboratory Final Screening Method".

[0044] Oncomelania: collected from the endemic area of ​​schistosomiasis in Yueyang, Hunan. The positive snails were removed by the method of group escape larvae, and cultured in the laboratory for 72 hours. Select snails with a snail age of 8 to 9 spins for later use.

[0045] Add 30ml of the different medicinal solutions ...

Embodiment 3

[0051] The total extract of ginkgolic acid is dissolved in a small amount of absolute ethanol, added with an appropriate emulsifier, and emulsified at a high speed to prepare an emulsion, and then diluted with boiled and cooled tap water to different concentrations: 40mg / L, 20mg / L, 10mg / L, 5mg / L, 1.25mg / L (the final concentration of ethanol is <0.1%). Niclosamide wettable powder is diluted with boiled and cooled tap water to different concentrations: 100ug / L, 50ug / L, 25ug / L, 12.5ug / L, 6.25ug / L, 3.13ug / L. At the same time, boiled and cooled tap water was used as a blank control.

[0052] The molluscicide test was carried out according to the immersion method in the WHO "Molluscicide Laboratory Final Screening Method".

[0053] Oncomelania: collected from the endemic area of ​​schistosomiasis in Yueyang, Hunan. The positive snails were removed by the method of group escape larvae, and cultured in the laboratory for 72 hours. Select snails with a snail age of 8 to 9 spins for...

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PUM

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Abstract

The present invention belongs to the field of biological pesticide, and is especially one kind of high efficiency and low toxicity molluscacide containing ginkgolic acid. The present invention adopts ginkgolic acid as main component, and niclosamide, copper ion and supplementary material to compound composite molluscacide for killing water snail as the unique intermediate host of blood flukes and preventing propagation of schistosomiasis. The test shows that the molluscacide has fast water snail killing effect, no damage to human body and no obvious toxicity on fresh water fish. The molluscacide may be prepared into concentrated liquid preparation or solid preparation for easy production, storing, transportation and use.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and relates to a high-efficiency and low-toxic molluscicide preparation containing ginkgolic acid, which is mainly used for killing snails, the only intermediate host of schistosomiasis, and preventing the spread of schistosomiasis. Background technique [0002] The miracidia of schistosomiasis can develop into schistosomiasis after invading the snail, which multiplies tens of thousands of times in the snail, and continuously produces cercariae in the water or on the wet soil surface, so as to infect humans and other animals. The spread and spread of the disease. Oncomelania hupensis (O. hupensis) is the only intermediate host for spreading schistosomiasis, which is widespread in my country. Elimination of snails can cut off the transmission route of schistosomiasis. Therefore, killing snails is an internationally recognized important measure to prevent schistosomiasis. [0003] Schistosom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/20A01P9/00A01N37/06A01N37/48
Inventor 潘家祜毛佐华俞培忠
Owner FUDAN UNIV
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