Cat foetal trichomonas lfd-rpa detection kit and method
By designing specific primers and probes, the LFD-RPA technology solves the problems of insufficient sensitivity and cumbersome operation in the detection of feline fetal trichomonas, achieving rapid, sensitive, and visualized detection results, which are suitable for on-site clinical testing in pets.
Patent Information
- Application Number
- CN202610799891.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-06-04
- Publication Date
- 2026-07-17
AI Technical Summary
Existing methods for detecting feline fetal trichomonas are insufficiently sensitive, cumbersome, and time-consuming. They lack rapid, sensitive, and clinically applicable detection technologies, and traditional methods are subject to the risk of nucleic acid contamination and dependence on sophisticated equipment.
Specific primer pairs TF-LFD-F/TF-LFD-R and probe TF-LFD-Probe were designed, and recombinase polymerase amplification (RPA) technology and lateral flow chromatography (LFD) strips were used to amplify nucleic acids under isothermal conditions of 37–42℃. The results were interpreted using lateral flow chromatography strips. A dual-labeling system was used to avoid non-specific amplification.
It achieves detection within 40 minutes with a sensitivity of 1.50 copies/μL, which is significantly better than traditional microscopic examination and ordinary PCR. The results are intuitive and reliable, and no precision temperature-controlled instruments are required, making it suitable for on-site, real-time testing in frontline veterinary clinics.
Smart Images
Figure CN122405863A_ABST