36 results about "Pseudomonas monteilii" patented technology

The invention provides a kind of Pseudomonas montelii and its application, the Pseudomonas montelii PM-41 preservation number is CGMCC No.18491; ‑41 was activated, fermented and cultured and applied to the roots of Panax notoginseng. The Pseudomonas montellae PM-41 provided by the present invention has good disease prevention and growth promotion effects, can antagonize the Panax notoginseng rust bacteria, reduce the occurrence of Panax notoginseng rust, and at the same time can degrade the autotoxic substances secreted by Panax notoginseng , especially for saponins Rg1 and Rb1, the concentration is greatly reduced after 192 hours of inoculation, and the degradation rate reaches more than 15%; The emergence rate is above 20%, which obviously relieves the obstacles to continuous cropping.

A kind of Pseudomonas monteilii and its application belong to the technical field of agricultural fertilizers. The Pseudomonas monteilii PN1 screened by the present invention provides a new microbial resource, which has multiple biological functions, It can not only degrade the residual p-nitrophenol in the soil, but also improve the tolerance and stress resistance of corn seedlings to abiotic stresses such as pesticide residues, drought and saline-alkali, which is of great significance for ensuring the safe, green and efficient production of corn and other crops Important guiding significance and application value.

The invention belongs to the biology engineering technical field, and relates to a method for preparing indigo by conversing indole through phenol-degrading bacteria. The invention is characterized in that the phenol-degrading microbial strain capable of effectively catalyzing indole to synthesize indigo is employed, and is Pseudomonas monteilii QM, and the preservation number is CGMCC No.5054; when the biotransformation reaction is carried out, the cell dry weight concentration of the Pseudomonas monteilii QM bacterium in a reaction solution is 0.1-10g / L, the indole concentration is 25-200mg / L, under temperature of 20-40 DEG C, the shaking table rotating speed is controlled with 50-300r / min, and the oscillation reaction lasts for 1-8 hours. The condition of the biotransformation reaction is mild, the product concentration reaches as high as 42.5mg / L, the production period is short, the cost is low, the extraction step is simple, the production step is clean, and the method is suitable for industrial production and application.

The invention clones, expresses and discloses a monooxygenase PMO-6814 from pseudomonas monteilii as well as application thereof, and particularly relates to a genetically engineered bacterium which has the activity of a monooxygenase and is prepared by cloning the encoding gene of the monooxygenase PMO-6814 from a pseudomonas monteilii genome, constructing an expression vector of the encoding gene and guiding the expression vector into escherichia coli. The monooxygenase protein has the amino acid sequence as shown in SEQ ID No.1, and the corresponding encoding gene of the monooxygenase protein has the nucleotide sequence as shown in SEQ ID No.2. After being reconstructed and expressed, the monooxygenase protein is capable of effectively catalyzing a thioether substrate to generate sulfoxide, has relatively high conversion efficiency and stereoisomeride enantioselectivity, and can be used for the biosynthesis of chiral sulfoxide medicines.