Pseudomonas monteilii psm006 and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas monteilii psm006 and application thereof. The pseudomonas monteilii psm006 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 3, 2025, the address of the pseudomonas monteilii psm006 is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number of the pseudomonas monteilii psm006 is CGMCC NO.33294. The invention provides the pseudomonas monteilii psm006, the pseudomonas monteilii psm006 can survive in a certain saline-alkaline environment, has good silicate dissolving capacity and certain phosphorus dissolving and nitrogen fixing capacity, and in addition, the pseudomonas monteilii psm006 can secrete auxin and the like to improve the germination rate of Chinese cabbage seeds in the saline-alkaline environment.

Description

Technical Field

[0001] This application belongs to the field of microbial technology, and specifically relates to a Pseudomonas monteilii psm006 and its application. Background Art

[0002] Currently, due to human land reclamation, over-irrigation, and other factors related to climate change, more than 6% of the total land area in the world is affected by salinization. Salinization has a very serious impact on both soil and plants. Saline-alkali soil is characterized by high salt content and strong alkalinity, which reduces the water content in the soil and the soil air permeability, showing the phenomenon of "muddy when wet and hard when dry". There are two main reasons for the harm caused by soil salinization: the high concentration of salt ions in the soil leads to an imbalance in the osmotic pressure of the plants, thereby reducing the absorption of water by the roots and causing internal dehydration, and its effect is similar to the dehydration caused by drought. At the same time, the high concentration of salt ions also inhibits the absorption of nutrients such as phosphorus, potassium, silicon, and nitrogen by plants. In addition, the accumulation of salt ions in the soil leads to ionic toxicity, directly interfering with the growth and metabolic process of plant cells, and thus affecting plant growth.

[0003] The existing methods for improving saline-alkali soil mainly include physical methods, chemical methods, and microbial methods. Subsurface pipe drainage in physical methods is a physical method with relatively high feasibility and good treatment effect among existing methods. Subsurface pipes are laid in the soil layer at a certain depth, and rainwater or irrigation fresh water is used to dissolve and drain the salts in the soil, so as to achieve the purpose of reducing the salt concentration in the soil. However, this method has high labor and material costs and is not very economical and environmentally friendly. And if the chemical modifiers used in chemical methods are added excessively, it will cause serious environmental pollution. In order for crops to grow continuously and healthily in saline-alkali land, it is also necessary to often add chemical fertilizers additionally to meet the nutritional needs of crops. Chemical fertilizers will remain in the soil for a long time, which will have a long-term impact on the environment, affecting soil fertility, crop health, and carbon footprint, and causing soil eutrophication and heavy metal accumulation, ultimately harming human health. Therefore, there is an urgent need to develop a green improvement method. Summary of the Invention

[0004] The purpose of the present invention is to solve the deficiencies of the prior art and provide a Pseudomonas monteilii psm006 and its application. The following technical solutions are specifically adopted: In the first aspect, the present invention provides a Pseudomonas monteilii psm006, and this Pseudomonas monteilii ( Pseudomonas monteilii ) psm006 was deposited at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on January 3, 2025. The address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO. 33294.

[0005] In the present invention, a highly efficient silicon-solubilizing strain, Pseudomonas monteilii psm006, was screened from the soil. This strain also has certain salt and alkali tolerance capabilities and growth-promoting capabilities. In the present invention, a salt environment was simulated to study the effects of this strain on Chinese cabbage seeds. The results showed that PSM006 can promote the germination rate of Chinese cabbage seeds in a salt environment.

[0006] The gene sequence of the 16S rDNA of the above-mentioned Pseudomonas monteilii psm006 is shown in SEQ ID No.1.

[0007] SEQ ID No.1:

[0008] In a second aspect, the present invention provides the use of the above-mentioned Pseudomonas monteilii psm006 or its bacterial solution in promoting the germination of Chinese cabbage seeds and / or preparing a Chinese cabbage seed germination promoter.

[0009] In a third aspect, the present invention provides a Chinese cabbage seed germination promoter, which comprises the above-mentioned Pseudomonas monteilii psm006.

[0010] In a fourth aspect, the present invention provides the use of the above-mentioned Pseudomonas monteilii psm006 or its bacterial solution in degrading silicate and / or preparing a silicate degrading agent.

[0011] In a fifth aspect, the present invention provides a silicate degrading agent, which comprises the above-mentioned Pseudomonas monteilii psm006.

[0012] In a sixth aspect, the present invention provides the use of the above-mentioned Pseudomonas monteilii psm006 or its bacterial solution in inhibiting plant pathogens and / or preparing a plant pathogen inhibitor.

[0013] As a further preferred embodiment, the above-mentioned plant pathogens include the pathogen of maize stalk rot or the pathogen of wheat take-all.

[0014] In a seventh aspect, the present invention provides a plant pathogen inhibitor, which comprises the above-mentioned Pseudomonas monteilii psm006 or its bacterial solution.

[0015] In an eighth aspect, the embodiments of the present application provide a method for inhibiting plant pathogens, which uses the above-mentioned Pseudomonas monteilii psm006 or its bacterial solution to treat plants, and the plant pathogens include the pathogen of maize stalk rot or the pathogen of wheat take-all.

[0016] The beneficial effects of the present invention are as follows: The present invention provides a strain of Pseudomonas monteilii psm006, which can survive in a certain saline-alkali environment, has good ability to dissolve silicate, and also has certain phosphorus-solubilizing and nitrogen-fixing abilities. In addition, the strain can secrete auxin and other substances to improve the germination rate of Chinese cabbage seeds in saline environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention, and those of ordinary skill in the art can also obtain other drawings based on these drawings without creative efforts.

[0018] Figure 1The colony morphology (a) and Gram staining (b) of Pseudomonas monteilii psm006 are shown, scale bar: 1 μm; Figure 2 The phylogenetic tree map of Pseudomonas monteilii psm006 is shown; Figure 3 The growth of Pseudomonas monteilii psm006 on the silicon-solubilizing plate (a) and the silicon-solubilizing hydrolysis zone (the dashed part in b) are shown; Figure 4 The determination of the silicon-solubilizing ability of Pseudomonas monteilii psm006 in liquid is shown; Figure 5 The growth of Pseudomonas monteilii psm006 in LB liquid medium containing different concentrations of NaCl is shown; Figure 6 The growth of Pseudomonas monteilii psm006 in LB liquid medium with different pH values is shown; Figure 7 The phosphorus-solubilizing hydrolysis zone of Pseudomonas monteilii psm006 is shown; Figure 8 The nitrogen-fixing hydrolysis zone of Pseudomonas monteilii psm006 is shown; Figure 9 The size of the silicon-solubilizing hydrolysis zone of Pseudomonas monteilii psm006 under different saline-alkali conditions is shown: (a) under mild (3% NaCl) saline-alkali environmental conditions; (b) under moderate (6% NaCl) saline-alkali environmental conditions. The red solid line in the figure is the colony, and the red dashed line is the silicon-solubilizing hydrolysis zone; Figure 10 The experimental results of Pseudomonas monteilii psm006 promoting the germination of Chinese cabbage seeds are shown, scale bar: 1 cm; Figure 11 The results of Pseudomonas monteilii psm006 inhibiting pathogenic bacteria are shown: (a) the pathogenic bacterium of maize stalk rot: Fusarium moniliforme ( Fusarium verticillioides ); (b) the pathogenic bacterium of wheat take-all: Gaeumannomyces graminis var. avenae( Gaeumonnomyces graminis var avenae ). Detailed implementation manners

[0019] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0020] Example 1 Screening and identification of Pseudomonas monteilii psm006 The experimental samples of the present invention were taken from the rhizosphere soil of plants collected from the saline-alkali land in Dalate Banner, Inner Mongolia. The specific implementation method is as follows: 1. Preparation of soil suspension: Weigh 10.0 g of soil sample and add it to a 250 mL shaking flask containing 90 mL of sterile water (aseptic operation during this process). Place it on a shaker and shake (180 rmp, 2 - 3 h) to obtain a soil suspension with a concentration of 10 -1 . Dilute the soil suspension to 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 by the 10-fold dilution method; 2. Purification of strains: Take 10 -4 , 10 -5 , 10 -6 dilution gradient suspensions, each 100 μL, and evenly spread them on the Aleksandrov solid medium (sucrose 5 g / L, Na 2 HPO 4 2 g / L, MgSO 4 ·7H 2 O 0.5 g / L, FeCl 3 ·6H 2 O 0.005 g / L, CaCO 3 0.1 g / L, magnesium silicate 2.5 g / L, agar 20 g / L) plates. Place them in a constant temperature incubator at 30°C for 2 - 3 days. Pick multiple single colonies with characteristics related to silicon-dissolving microorganisms and purify them by streaking on the Aleksandrov solid plates, repeating 2 - 3 times.

[0021] 3. Strain identification (1) Gram staining Screen out the single strain by the dilution plate coating method and streak it on an LB solid plate (NaCl 10 g / L, peptone 10 g / L, yeast extract 5 g / L, agar 1.5 - 2.0 g / L), and culture it in an incubator at 30°C for 24 h to observe the colony morphology. Inoculate it into a liquid LB medium (NaCl 10 g / L, peptone 10 g / L, yeast extract 5 g / L). Take 10 μL of the bacterial liquid and drop it on a sterile glass slide. Slightly heat it over the flame of an alcohol lamp to fix the strain. After the glass slide cools, drop the crystal violet solution until it covers the colony and stain for about 1 min; gently rinse off the stain under tap water and dry it with absorbent paper; drop the iodine solution until it covers the colony and stain for about 1 min; rinse off the stain in the same way and dry it; drop 95% anhydrous ethanol to decolorize until the colony becomes colorless, wash with water; drop the safranin solution until it covers the colony and stain for about 1 min, rinse and dry in the same way, and then observe it under a microscope.

[0022] The results are as follows Figure 1 as shown: The colonies of strain psm006 on the LB solid plate are white, smooth, opaque, with neat edges, convex in the center, 1 mm in diameter. The result of Gram staining is purplish red, belonging to Gram-negative bacteria. Under the microscope, the cells of psm006 are rod-shaped or slightly curved.

[0023] (2)Molecular biology identification The genomic DNA of the strain was extracted using a bacterial DNA extraction kit (Sangon Biotech (Shanghai) Co., Ltd.) and used as a template. The screened strain was subjected to PCR amplification using the 16S rDNA universal primer sequences 27F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-GGTTACCTTGTTACGACTT- 3’) (the amplification system is shown in Table 1). After completion, it was sent to Shanghai Jieli Biotechnology Co., Ltd. for sequencing, and the sequencing results are shown in SEQ ID No.1.

[0024] Table 1 PCR system The obtained sequence was submitted to NCBI for Blast sequence alignment, and a phylogenetic tree was constructed using the mega11 software (Molecular Evolutionary Genetics Analysis).

[0025] The results showed that: as Figure 2 shown, it can be identified as Pseudomonas through the phylogenetic tree of the strain, so it was named Pseudomonas monteilii ( Pseudomonas monteilii ).

[0026] Example 2 The silicate-solubilizing ability of Pseudomonas monteilii psm006 screened in Example 1 was detected, and the specific process is as follows: (1)Qualitative detection by plate method: Take 10 μL of the bacterial liquid on the magnesium silicate plate (glucose 10 g / L, magnesium silicate 2.5 g / L, agar 1.5 g / L) and observe the size of the hydrolysis zone. The results are as Figure 3 shown. The strain can produce a clear hydrolysis zone on this plate, indicating its silicate-solubilizing ability. (2)Quantitative detection by liquid method: Inoculate into Aleksandrov liquid medium at an inoculation amount of 1%, culture at 30 °C and 200 rmp for 5 days, centrifuge the culture solution at 8000 rmp for 15 min, take the supernatant for filtration, and detect the soluble silicon content in the supernatant with an ICP-MS (Inductively coupled plasma-Mass Spectrometry) machine. The results are as Figure 4 shown. Compared with the blank control CK group without adding the strain, the soluble silicon content in the supernatant of the culture solution after adding the strain increased significantly, reaching a significant difference.

[0027] Example 3 Detection of salt and alkali tolerance and growth promotion ability of Pseudomonas monteilii psm006 (1) Determination of salt tolerance Pick a single colony and incubate it overnight in LB liquid medium. Inoculate it at an inoculation amount of 1% into liquid LB media containing 1%, 3%, 5%, 6%, 7%, 8%, 9%, 10%, 11% NaCl (w / v) respectively, and culture it in a constant temperature shaker at 30 °C for 24 h. Use a spectrophotometer to measure the OD600 of the bacterial solution.

[0028] The results are as Figure 5 shown: As the NaCl gradient increases, the OD600 of the strain gradually decreases. Taking 6% NaCl as the critical value, when the NaCl concentration is 6% (w / v), the growth of the strain is inhibited, but there are still a relatively large number of cells that can grow. When NaCl is greater than 6% (w / v), the OD600 drops sharply below 1, indicating that the growth of the strain is extremely inhibited, suggesting that the maximum NaCl concentration the strain can tolerate is 6%.

[0029] (2) Determination of alkali tolerance Pick a single colony and incubate it overnight in LB liquid medium. Inoculate it at an inoculation amount of 1% into liquid LB media with pH = 7, 8, 9, 10, 11 respectively, and culture it in a constant temperature shaker at 30 °C for 24 h. Use a spectrophotometer to measure the OD 600 .

[0030] The results are as Figure 6 shown: The strain can grow and reproduce well when pH ≤ 9. When pH is greater than 9, the OD600 drops to almost 0, indicating that the highest alkaline environment the strain can tolerate is pH = 9.

[0031] (3) Determination of auxin (IAA) Pick a single colony and incubate it overnight in LB liquid medium; inoculate it into LB liquid medium containing L-tryptophan (200 mg / L) at an inoculation amount of 1%, and culture it on a shaker at 180 r / min and 30 °C for 36 h; pipette 2 mL of the bacterial liquid into a centrifuge tube, centrifuge at 10000 r / min for 10 min, take 1.5 mL of the supernatant and add an equal volume of Salkowski colorimetric solution (50 mL of 35% HC1O 4 + 1 mL of 0.5 mol / L FeCl 3 ) into a 5 mL centrifuge tube, let it stand in the dark for 30 min, and measure its OD530 value. Prepare a standard solution with analytical pure indoleacetic acid and perform a Salkowski colorimetric reaction to obtain a standard curve, and calculate the content of IAA in the fermentation broth per unit volume by referring to the standard curve.

[0032] The results showed that this strain (Pseudomonas monteilii psm006) could produce 11.5 ± 0.46 mg / L of indoleacetic acid, compared with only 8.0 mg / L in the negative control Escherichia coli DH5-α.

[0033] (4) Determination of siderophores Pick a single colony and incubate it overnight in LB liquid medium; inoculate it into MKB liquid medium at an inoculation amount of 1% and culture it at a constant temperature of 30 °C on a shaker for 36 h; take 1 mL of the bacterial liquid and centrifuge it at 12000 rpm for 10 min, take 100 μL of the supernatant and mix it with 100 μL of CAS quantitative detection solution, and perform the same operation with sterile ddH 2 O as a control. After reacting at room temperature for 1 h, record the OD630 values of the sample (As) and the control (Ar) respectively, and calculate the siderophore yield using the following formula: Siderophore unit (%) = 100 × (Ar - As) / Ar The results showed that the yield of siderophores secreted by this strain (Pseudomonas monteilii psm006) was 67.9 ± 1.02%, compared with only 14.9% in the negative control Escherichia coli DH5-α.

[0034] (5) Determination of the ability to produce ACC deaminase a. Pick a single colony into 30 mL of DF (KH 2 PO 4 4 g / L, Na 2 HPO 4 6 g / L, MgSO 4 ·7H 2 O 0.2 g / L, glucose 2 g / L, gluconic acid 2 g / L, citric acid 2 g / L, H 3 BO 3 0.01 mg / L, MnSO4 ·H 2 O 0.01 mg / L, ZnSO 4 ·7H 2 O 0.078 mg / L, CuSO 4 ·5H 2 O 0.05 mg / L, MoO 3 0.01 mg / L, FeSO 4 ·7H 2 O 1 mg / L) liquid medium, and cultured with dark shaking at 30 °C and 200 r / min for 12 h; b. Then, the bacterial liquid was centrifuged at 4 °C and 8000 r / min for 10 min, the bacterial suspension was collected, and the precipitate was resuspended again in 30 mL of ADF (DF medium added with 5 mmol / L ACC solution) liquid medium, and cultured with dark shaking at 30 °C and 200 r / min for 24 h; c. Centrifuged again at 4 °C and 8000 r / min for 10 min, resuspended with 5 mL of 0.1 moL / L Tris-HCl buffer (pH 7.6) and washed twice; transferred to a 1.5 mL centrifuge tube, resuspended the bacterial cells in 600 μL of 0.1 moL / L Tris-HCl buffer (pH 8.5) to dissolve the bacterial cells; added 30 μL of toluene and vortexed for 30 s to break the bacterial cells; d. Enzyme activity assay: Pipette 200 μL of the broken cell bacterial suspension, add 20 μL of 0.5 moL / L ACC solution, mix well and keep in a water bath at 30 °C for 15 min; e. Then add 1 mL of 0.56 moL / L HCl solution and shake well, centrifuge at 10000 r / min for 10 min; f. Take 1 mL of the above centrifuged supernatant, add 800 μL of 0.56 moL / L HCl solution, mix well and then add 300 μL of 2 g / L 2,4-dinitrophenylhydrazine solution, and keep in a water bath at 30 °C for 30 min; g. Add 2 mL of 2 moL / L NaOH solution for color development, and detect the OD540 value.

[0035] After deducting the spontaneous products in the control samples for each strain, calculate the OD540 value of the sample and substitute it into the standard curve to calculate the corresponding α-ketobutyric acid content. Defined as 1 enzyme activity unit for the amount of 1 μmoL α-ketobutyric acid formed per minute.

[0036] (6)Determination of cell protein concentration Take 10 μL of toluene-treated bacterial cells and dilute them 10-fold with 1×PBS; dilute the 1 mg / mL BSA protein standard solution with 1×PBS to obtain protein concentration gradients of 20 μg·mL -1 , 40 μg·mL -1 , 60 μg·mL -1 , 80 μg·mL -1 , 100 μg·mL -1 . Use 1×PBS as the blank control, with 3 replicates for each concentration; measure the protein concentration using the Bradford protein kit, and after reacting for 5 min, measure the OD595 value of each well with an enzyme-labeled instrument. Plot the standard curve with the average OD595 value on the vertical axis and the corresponding protein concentration on the horizontal axis; determine the protein concentration of the sample dilution based on the average OD595 value of the sample dilution on the standard curve.

[0037] The results showed that psm006 could produce ACC deaminase, and the enzyme activity reached 1.17 ± 0.35 U / mg. Using Escherichia coli DH5-α as the negative control, its enzyme activity was 0.0073 U / mg.

[0038] (7)Determination of phosphate-solubilizing ability Take 10 μL of the bacterial solution and inoculate it into the Mengjinna inorganic phosphorus medium (PKO) (glucose 10 g / L, (NH 4 ) 2 SO 4 0.5 g / L, KCl 0.3 g / L, NaCl 0.3 g / L, FeSO 4 · 7H 2 O 0.03 g / L, MgSO 4 ·7H 2 O 0.3 g / L, MnSO 4 · 4H 2 O 0.03 g / L, Ca 3 (PO 4 ) 2 10 g / L, agar 15 g / L) and culture it in an incubator at 30 °C for 5 - 7 d. If a clear hydrolysis zone appears, it indicates the ability to solubilize phosphorus.

[0039] The results are as Figure 7 shown: On the PKO plate, a clear hydrolysis zone appeared around the colonies, indicating that the strain has the ability to solubilize phosphorus.

[0040] (8)Determination of nitrogen-fixing ability Take 10 μL of the bacterial solution and inoculate it onto the Ashby nitrogen-free medium plate (KH 2 PO 40.2 g / L, NaCl 0.2 g / L, MgSO 4 0.2 g / L, CaCO 3 5 g / L, CaSO 4 0.1 g / L, glucose 10 g / L, agar powder 20 g / L) and cultured in an incubator at 30 °C for 5 - 7 d. If a transparent hydrolysis zone appears, it indicates the ability of nitrogen fixation.

[0041] The results are as Figure 8 shown: On the Ashby nitrogen - free medium plate, a transparent hydrolysis zone appeared around the colonies, indicating that the strain has the ability of nitrogen fixation.

[0042] Example 4 Verification of the silicon - dissolving ability of Pseudomonas monteilii psm006 under salt conditions Take 10 μL of the bacterial solution and place it on magnesium silicate plates under different conditions, and observe the size of the hydrolysis zone. Two gradients of NaCl were designed in the experiment: (1) Add 3% NaCl (w / v) additionally to the magnesium silicate medium; (2) Add 6% NaCl (w / v) additionally to the magnesium silicate medium.

[0043] The experimental results are as Figure 9 shown: It can be seen from the results that on the two groups of plates with additional NaCl (where a is 3% NaCl and b is 6% NaCl), the strain still has a certain silicon - dissolving ability, indicating that the strain has the potential for application in saline - alkali land improvement.

[0044] Example 5 Verification of the improvement of the germination rate of crops by Pseudomonas monteilii psm006 1. The effect of psm006 on the germination of Chinese cabbage seeds (1) Inoculate the strain with strong growth - promoting ability into Alexander liquid medium and culture it at 30 °C for 24 h, then centrifuge at 5000 rpm for 10 min; (2) Resuspend the cells with two concentrations of sodium chloride respectively to make their OD the same, obtaining a suspension; Design 6 treatments: (1) CK group: Use tap water as the blank control; (2) 3 g / L NaCl without bacteria; (3) 6 g / L NaCl without bacteria; (4) Add the strain psm006 additionally; (5) Add psm006 in 3 g / L NaCl; (6) Add psm006 in 6 g / L NaCl; Immerse the filter paper into the suspensions and liquids of the above different treatments respectively, and then put the surface - sterilized Chinese cabbage seeds and the filter paper together into a culture dish to form a "sandwich" structure, and place it in a suitable environment for 3 - 5 d; (3) Observe the seed germination situation.

[0045] The results are as follows Figure 10 shown: Salt stress inhibits the germination of Chinese cabbage seeds. The higher the salt concentration, the more obvious the inhibitory effect. After inoculation with the strain psm006, it can promote the germination of Chinese cabbage under salt stress and alleviate the damage caused by salt stress.

[0046] Example 6 Antibacterial experiment determination of Pseudomonas monteilii psm006 Use a sterile toothpick to pick up a 2×2 cm fungal block and place it upside down in the center of a PDA solid plate. Drop the same OD of an equal amount of the bacterial solution of psm006 at three corners of the plate, and observe the antibacterial results after culturing in an incubator at 26 °C for 5 d.

[0047] The results are as follows Figure 11 shown, and it can be seen from Figure 11 that the strain psm006 has a certain antagonistic effect on the pathogen of maize stalk rot: Fusarium moniliforme ( Fusarium verticillioides ) and the pathogen of wheat take-all: Gaeumannomyces graminis var. avenae( Gaeumonnomyces graminis var avenae ), and can inhibit the growth and reproduction of some pathogens, indicating that this strain has potential biocontrol ability.

[0048] The embodiments of the present application have been described above in conjunction with the accompanying drawings. Specific examples are used in this article to elaborate on the principle and implementation manner of the present application. The description of the above embodiments is only used to help understand the core idea of the present application. However, the present application is not limited to the above specific implementation manners. The above specific implementation manners are only illustrative and not restrictive. Under the inspiration of the present application, those of ordinary skill in the art can also make many forms without departing from the purpose of the present application and the scope protected by the claims, and all belong to the protection scope of the present application.

Claims

1. A strain of Pseudomonas monteridium psm006, characterized in that Pseudomonas monteri Pseudomonas monteilii )psm006 was deposited on January 3, 2025 in the General Microbiology Center of China Microorganism Culture Collection Administration, with the address at No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the deposit number CGMCC NO. 33294.

2. The Pseudomonas montegrioides psm006 according to claim 1, characterized in that The gene sequence of 16S rDNA of Pseudomonas montgomeryii psm006 is shown in SEQ ID No.

1.

3. Use of the Pseudomonas montgomeryii psm006 or its bacterial solution according to any one of claims 1 to 2 in promoting cabbage seed germination and / or preparing a cabbage seed germination promoter.

4. A cabbage seed germination promoter, characterized in that The cabbage seed germination promoter includes the Pseudomonas montgomeryii psm006 described in any one of claims 1-2.

5. Use of the Pseudomonas monteridium psm006 or its bacterial solution as described in any one of claims 1-2 in degrading silicon salts and / or preparing silicon salt degrading agents.

6. A silicon salt degradation agent, characterized in that: The silicon salt degradation agent includes the Pseudomonas montgomeryii psm006 described in any one of claims 1-2.

7. Use of the Pseudomonas monteridium psm006 or its bacterial solution according to any one of claims 1 to 2 in inhibiting plant pathogens and / or preparing plant pathogen inhibitors.

8. The use according to claim 7, characterized in that: The plant pathogens include corn stalk rot pathogens or wheat take-all pathogens.

9. A plant pathogen inhibitor, characterized in that: The plant pathogen inhibitor comprises the Pseudomonas monteridium psm006 or its bacterial solution as described in any one of claims 1-2.

10. A method for inhibiting plant pathogens, characterized in that: The plants are treated with the Pseudomonas montoni psm006 or its bacterial solution as described in any one of claims 1-2, wherein the plant pathogens include pathogens of corn stalk rot or wheat take-all disease.

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